First-order transitions for some generalized XY models

In this note we demonstrate the occurrence of first-order transitions in temperature for some recently introduced generalized XY models, and also point out the connection between them and annealed site-diluted (lattice-gas) continuous-spin models

Two Case Reports of Biliary Tract Injuries during Laparoscopic Cholecystectomy

Background and Study Aims. Biliary tract injuries (BTI) represent the most serious and potentially life-threatening complication of cholecystectomy occurring also during laparoscopic approaches. Patients and Methods. We describe and discuss two different cases of BTI occurring during laparoscopic cholecystectomy (LC). Results. Two patients developed BTI during LC and one evidenced the complication during the LC itself and was treated during the same LC in real time. The other patient evidenced BTI only after the primary intervention and was successfully reoperated in laparotomy after 10 days from the LC. Conclusions. The factors that predispose to the occurrence of BTI during cholecystectomy and the cautions to be used to prevent BTI are discussed

Raman microprobe characterization of electrodeposited S-rich CuIn(S,Se)2 for photovoltaic applications: Microstructural analysis

This article reports a detailed Raman scattering and microstructural characterization of S-rich CuIn(S,Se)2 absorbers produced by electrodeposition of nanocrystalline CuInSe2 precursors and subsequent reactive annealing under sulfurizing conditions. Surface and in-depth resolved Raman microprobe measurements have been correlated with the analysis of the layers by optical and scanning electron microscopy, x-ray diffraction, and in-depth Auger electron spectroscopy. This has allowed corroboration of the high crystalline quality of the sulfurized layers. The sulfurizing conditions used also lead to the formation of a relatively thick MoS2 intermediate layer between the absorber and the Mo back contact. The analysis of the absorbers has also allowed identification of the presence of In-rich secondary phases, which are likely related to the coexistence in the electrodeposited precursors of ordered vacancy compound domains with the main chalcopyrite phase, in spite of the Cu-rich conditions used in the growth. This points out the higher complexity of the electrodeposition and sulfurization processes in relation to those based in vacuum deposition techniques

Suppression of SERK gene expression affects fungus tolerance and somatic embryogenesis in transgenic lettuce.

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A cellular disease model toward gene therapy of TGM1-dependent lamellar ichthyosis

Lamellar ichthyosis (LI) is a chronic disease, mostly caused by mutations in the TGM1 gene, marked by impaired skin barrier formation. No definitive therapies are available, and current treatments aim at symptomatic relief. LI mouse models often fail to faithfully replicate the clinical and histopathological features of human skin conditions. To develop advanced therapeutic approaches, such as combined ex vivo cell and gene therapy, we established a human cellular model of LI by efficient CRISPR-Cas9-mediated gene ablation of the TGM1 gene in human primary clonogenic keratinocytes. Gene-edited cells showed complete absence of transglutaminase 1 (TG1) expression and recapitulated a hyperkeratotic phenotype with most of the molecular hallmarks of LI in vitro. Using a self-inactivating γ-retroviral (SINγ-RV) vector expressing transgenic TGM1 under the control of its own promoter, we tested an ex vivo gene therapy approach and validate the model of LI as a platform for pre-clinical evaluation studies. Gene-corrected TGM1-null keratinocytes displayed proper TG1 expression, enzymatic activity, and cornified envelope formation and, hence, restored proper epidermal architecture. Single-cell multiomics analysis demonstrated proviral integrations in holoclone-forming epidermal stem cells, which are crucial for epidermal regeneration. This study serves as a proof of concept for assessing the potential of this therapeutic approach in treating TGM1-dependent LI

Jets, knots and tails in planetary nebulae: NGC 3918, K 1-2 and Wray 17-1

We analyze optical images and high-resolution, long-slit spectra of three planetary nebulae which possess collimated, low-ionization features. NGC 3918 is composed of an inner, spindle-shaped shell mildly inclined with respect to the plane of the sky. Departing from the polar regions of this shell, we find a two-sided jet expanding with velocities which increase linearly with distance from 50 to 100 km/s. The jet is probably coeval with the inner shell (with the age of approximately 1000 D yr, where D is the distance in kpc), suggesting that its formation should be ascribed to the same dynamical processes which also shaped the main nebula, and not to a more recent mass loss episode. We discuss the formation of the aspherical shell and jet in the light of current hydrodynamical and magnetohydrodynamical theories. K 1-2 is a planetary nebula with a close binary nucleus which shows a collimated string of knots embedded in a diffuse, elliptical shell. The knots expand with a velocity similar to that of the elliptical nebula (25 km/s), except for an extended tail located out of the main nebula, which linearly accelerates up to 45 km/s. We estimate an inclination on the line of the sight of 40 degres for the string of knots; once the orientation of the orbit is also determined, this information will allow us to test the prediction of current theories of the occurrence of polar jets from close binary systems. Wray 17-1 has a complex morphology, showing two pairs of low-ionization structures located in almost perpendicular directions from the central star, and embedded in a large, diffuse nebula. The two pairs show notable similarities and differences, and their origin is very puzzling.Comment: 20 pages plus 10 figures. ApJ recently published (ApJ 523, 721 (1999)

Dynamic Responsive Inguinal Scaffold Activates Myogenic Growth Factors Finalizing the Regeneration of the Herniated Groin

Background: Postoperative chronic pain caused by fixation and/or fibrotic incorporation of hernia meshes are the main concerns in inguinal herniorrhaphy. As inguinal hernia is a degenerative disease, logically the treatment should aim at stopping degeneration and activating regeneration. Unfortunately, in conventional prosthetic herniorrhaphy no relationship exists between pathogenesis and treatment. To overcome these incongruences, a 3D dynamic responsive multilamellar scaffold has been developed for fixation-free inguinal hernia repair. Made of polypropylene like conventional flat meshes, the dynamic behavior of the scaffold allows for the regeneration of all typical inguinal components: connective tissue, vessels, nerves, and myocytes. This investigation aims to demonstrate that, moving in tune with the groin, the 3D scaffold attracts myogenic growth factors activating the development of mature myocytes and, thus, re-establishing the herniated inguinal barrier. Methods: Biopsy samples excised from the 3D scaffold at different postoperative stages were stained with H&E and Azan–Mallory; immunohistochemistry for NGF and NGFR p75 was performed to verify the degree of involvement of muscular growth factors in the neomyogenesis. Results: Histological evidence of progressive muscle development and immunohistochemical proof of NFG and NFGRp75 contribution in neomyogenesis within the 3D scaffold was documented and statistically validated. Conclusion: The investigation appears to confirm that a 3D polypropylene scaffold designed to confer dynamic responsivity, unlike the fibrotic scar plate of static meshes, attracts myogenic growth factors turning the biological response into tissue regeneration. Newly developed muscles allow the scaffold to restore the integrity of the inguinal barrier

Dissociative Photoionization of Methyl Thiochloroformate, ClC(O)SCH3, following Sulfur 2p, Chlorine 2p, Carbon 1s and Oxygen 1s Excitations

The electronic transitions and the dissociativeionic photoionization mechanisms of gaseous ClC(O)SCH3have been investigated at the VUV and soft X-ray energyregions of S 2p, Cl 2p, C 1s, and O 1s core edges using tunablesynchrotron radiation and time-of-flight mass spectrometry.The relative abundances of the ionic fragments were obtainedfrom both PEPICO (photoelectron photoion coincidence)and PEPIPICO (photoelectron photoion photoion coincidence)spectra. The presence of a moderate site- and element-specific fragmentation effects and its implication regardingchemical reactions were analyzed. The relationship of the current results with the interstellar chemistry is also a goal of this pieceof work.Fil: Geronés, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaFil: Erben, Mauricio Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaFil: Romano, Rosana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaFil: Cavasso Filho, Reinaldo L.. Universidade Federal do ABC; BrasilFil: Della Védova, Carlos O.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentin

Climatology of GPS ionospheric scintillations over high and mid-latitude European regions

We analyze data of ionospheric scintillation in the geographic latitudinal range 44°–88° N during the period of October, November and December 2003 as a first step to develop a “scintillation climatology” over Northern Europe. The behavior of the scintillation occurrence as a function of the magnetic local time and of the corrected magnetic latitude is investigated to characterize the external conditions leading to scintillation scenarios. The results shown herein, obtained merging observations from four GISTM (GPS Ionospheric Scintillation and TEC Monitor), highlight also the possibility to investigate the dynamics of irregularities causing scintillation by combining the information coming from a wide range of latitudes. Our findings associate the occurrences of the ionospheric irregularities with the expected position of the auroral oval and ionospheric troughs and show similarities with the distribution in magnetic local time of the polar cap patches. The results show also the effect of ionospheric disturbances on the phase and the amplitude of the GPS signals, evidencing the different contributions of the auroral and the cusp/cap ionosphere