69 research outputs found
Photo-induced chemistry for the design of oligonucleotide conjugates and surfaces
A photocaged diene is introduced at the 5â˛-end of oligonucleotides using the H-phosphonate approach. The photoenol-functionalized DNA is subsequently employed for the conjugation to a protein and the spatially controlled immobilization onto surfaces using a light-induced DielsâAlder cycloaddition. Fully functional proteinâDNA conjugates and patterned DNA surfaces are obtained under mild irradiation conditions
Hetero-Diels-Alder-Cycloaddition mit RAFT-Polymeren als Biokonjugationsplattform
Wir stellen die Biokonjugation von Polymeren vor, die durch RAFTâPolymerisation mittels HeteroâDielsâAlderâCycloaddition durch ihren inhärenten terminalen Thiocarbonylthiorest mit einem dienmodifizierten Modellprotein synthetisiert wurden und keine spezifische funktionelle Endgruppe tragen. Die quantitative Konjugation erfolgt im Verlauf einiger Stunden bei Raumtemperatur und nahezu neutralem pHâWert und in Abwesenheit jeglichen Katalysators. Unsere Technologieplattform liefert thermoresponsive Biokonjugate, deren Aggregation allein durch die Polymerketten gesteuert wird
Hetero-DielsâAlder Cycloaddition with RAFT Polymers as Bioconjugation Platform
We introduce the bioconjugation of polymers synthesized by RAFT polymerization, bearing no specific functional end group, by means of heteroâDielsâAlder cycloaddition through their inherent terminal thiocarbonylthio moiety with a dieneâmodified model protein. Quantitative conjugation occurs over the course of a few hours, at ambient temperature and neutral pH, and in the absence of any catalyst. Our technology platform affords thermoresponsive bioconjugates, whose aggregation is solely controlled by the polymer chains
The electronic effects on the formation of N-arylmaleimides and isomaleimides
The synthesis of a range of aromatic maleimides and isomaleimides is reported. The effect of different substituents on the aromatic system and the products formed is also discussed
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Flavin-conjugated iron oxide nanoparticles as enzyme-inspired photocatalysts for Azo Dye degradation
In this work, a new photocatalytic system consisting of iron oxide nanoparticles (IONPs), coated with a catechol-flavin conjugate (DAFL), is synthesized and explored for use in water remediation. In order to test the efficiency of the catalyst, the photodegradation of amaranth (AMT), an azo dye water pollutant, was performed under aerobic and anaerobic conditions, using either ethylenediaminetetraacetic acid (EDTA) or 2-(N-morpholino)ethanesulfonic acid (MES) as electron donors. Depending on the conditions, either dye photoreduction or photooxidation were observed, indicating that flavin-coated iron-oxide nanoparticles can be used as a versatile enzyme-inspired photocatalysts.Funded by EPSRC DTC Studentship, grant number EP/N509620/
Detection of DNA probes using Diels Alder cycloaddition and SERRS
A number of methods for detecting specific DNA sequences have been used to provide data for use in diagnosis of disease states and examination of gene expression. This study shows how the use of labelled oligonucleotides created by Diels Alder cycloaddition can be used as surface enhanced resonance Raman scattering (SERRS) active probes that provide easily identifiable signals at low concentrations in a mixture. The probes were produced by first tagging the oligonucleotides with a furan residue at the 5'-terminus to act as the diene. Three specifically designed benzotriazole azo maleimide dyes were then used as dienophiles to undergo cycloaddition with the furan modified oligonucleotide to generate SERRS active probes. These probes gave excellent SERRS signals from a silver-PVA film. Surface mapping of the silver PVA film indicated that the distribution of the dyes was uniform and that the number of moles of probe detected at any one time was approximately in the attomole region
A new approach to oligonucleotide labelling using Diels-Alder cycloadditions and detection by SERRS
Diels-Alder cycloaddition has been used to add a benzotriazole azo dye to a diene tagged oligonucleotide to generate unique SERRS signals at 10 attomoles
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