Thin Ohmic or superconducting strip with an applied ac electric current

The complex impedance, currents, and electric and magnetic fields are calculated as functions of resistivity and frequency or London depth for a long thin strip with applied ac current. Both Ohmic and superconducting strips are considered. While the inductance per unit length of the strip depends on the strip length logarithmically, the sheet current, magnetic field, resistance, and magnetic susceptibility are independent of this length. It is found that the enhancement of resistance by the skin effect in thin Ohmic strips is much weaker (logarithmic) than in thick wires.Comment: 4 pages, 3 figures, for Phys. Rev.

Randomized Dynamical Decoupling Techniques for Coherent Quantum Control

The need for strategies able to accurately manipulate quantum dynamics is ubiquitous in quantum control and quantum information processing. We investigate two scenarios where randomized dynamical decoupling techniques become more advantageous with respect to standard deterministic methods in switching off unwanted dynamical evolution in a closed quantum system: when dealing with decoupling cycles which involve a large number of control actions and/or when seeking long-time quantum information storage. Highly effective hybrid decoupling schemes, which combine deterministic and stochastic features are discussed, as well as the benefits of sequentially implementing a concatenated method, applied at short times, followed by a hybrid protocol, employed at longer times. A quantum register consisting of a chain of spin-1/2 particles interacting via the Heisenberg interaction is used as a model for the analysis throughout.Comment: 7 pages, 2 figures. Replaced with final version. Invited talk delivered at the XXXVI Winter Colloquium on the Physics of Quantum Electronics, Snowbird, Jan 2006. To be published in J. Mod. Optic

Transferrin-polycation-DNA complexes. The effect of polycations on the structure of the complex and DNA delivery to cells.

We have previously described a gene delivery system based upon the receptor-mediated endocytosis of DNA complexed with transferrin-polycation conjugates. This delivery system has been found to be very effective for both the internalization and the expression of genetic material in cells that have many transferrin receptors. Upon scrutinization of the parameters involved in this method, which we have termed transferrinfection, we note two important features of the process: the polycation in polycation-transferrin conjugates, as expected, serves to attach the transferrin moiety to the DNA and, in addition, the polycation functions to condense the DNA into a doughnut structure. Electron microscopic analysis of a range of poorly active to highly active transferrinfection samples reveals a strong correlation between DNA condensation and cellular DNA uptake. Furthermore, we demonstrate that the transfection activity of the DNA complex can be increased by addition of free polycation as long as a sufficient quantity of polycation-transferrin conjugates remains in the complex to ensure its binding to the cellular receptor

Net ecosystem productivity and its uncertainty in a diverse boreal peatland

Net ecosystem exchange (NEE) of CO2 was measured in four peatlands along plant community, hydrologic, and water chemistry gradients from bog to rich fen in a diverse peatland complex near Thompson, Manitoba, as part of the Boreal Ecosystem-Atmosphere Study (BOREAS). A simple model for estimating growing season net ecosystem productivity (NEP) using continuous measurements of photosynthetically active radiation (PAR), and peat temperature was constructed with weekly chamber measurements of NEE from May to October 1996. The model explained 79–83% of the variation in NEE across the four sites. Model estimation and parameter uncertainty calculations were performed using nonlinear regression analyses with a maximum likelihood objective function. The model underestimated maximum NEE and respiration during the midseason when the standard errors for each parameter were greatest. On a daily basis, uncertainty in the midday NEE simulation was higher than at night. Although the magnitude of both photosynthesis and respiration rates followed the trophic gradient bog less than poor fen less than intermediate fen less than rich fen, NEP did not follow the same pattern. NEP in the bog and rich fen was close to zero, while the poor and intermediate fens had higher NEP due to a greater imbalance between uptake and release of CO2. Although all sites had a positive growing season NEP, upper and lower 95% confidence limits showed that the bog and rich fen were either a source or sink of CO2 to the atmosphere, while the sedge-dominated poor and intermediate fens were accumulating approximately 20–100 g CO2 C m−2over the 5 month period in 1996. Peatland ecosystems may switch from a net sink to a source of carbon on short timescales with small changes in soil temperature or water table position. Since the difference between production and decomposition is small, it is important to understand and quantify the magnitude of uncertainty in these measurements in order to predict the effect of climatic change on peatland carbon exchange

Advanced ablation strategies for management of post-surgical atrial arrhythmias.

Post-surgical arrhythmias include a wide range of arrhythmias occurring late after cardiac surgery and represent a complex substrate for catheter ablation either because of extended scar and remodeling or because of limited access to the area of interest. Novel image integration and ablation tools have made the catheter ablation in this population both feasible and successful. We review a structured approach to catheter ablation of post-surgical atrial arrhythmias in various patient cohorts including the most common congenital heart defects

What to Fix? Distinguishing between design and non-design rules in automated tools

Technical debt---design shortcuts taken to optimize for delivery speed---is a critical part of long-term software costs. Consequently, automatically detecting technical debt is a high priority for software practitioners. Software quality tool vendors have responded to this need by positioning their tools to detect and manage technical debt. While these tools bundle a number of rules, it is hard for users to understand which rules identify design issues, as opposed to syntactic quality. This is important, since previous studies have revealed the most significant technical debt is related to design issues. Other research has focused on comparing these tools on open source projects, but these comparisons have not looked at whether the rules were relevant to design. We conducted an empirical study using a structured categorization approach, and manually classify 466 software quality rules from three industry tools---CAST, SonarQube, and NDepend. We found that most of these rules were easily labeled as either not design (55%) or design (19%). The remainder (26%) resulted in disagreements among the labelers. Our results are a first step in formalizing a definition of a design rule, in order to support automatic detection.Comment: Long version of accepted short paper at International Conference on Software Architecture 2017 (Gothenburg, SE

DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety

We have previously demonstrated that transferrin-polycation conjugates are efficient carrier molecules for the introduction of genes into eucariotic cells. We describe here a more specific method for conjugation of transferrin with DNA-binding compounds involving attachment at the transferrin carbohydrate moiety. We used the polycation poly(L-lysine) or the DNA intercalator, ethidium homodimer as DNAbinding domains. Successful transferrin-receptor-mediatedd elivery and expression of the Photinus pyralis luciferase gene in K562 cells has been shown with these new transferrin conjugates. The activity of the transferrin-ethidium homodimer (TfEtD) conjugates is low relative to transferrin-polylysine conjugates; probably because of incomplete condensation of the DNA. However, DNA delivery with TfEtD is drastically improved when ternary complexes of the DNA with TfEtD and the DNA condensing agent polylysine are prepared. The gene delivery with the carbohydrate-linked transferrin-polylysine conjugates is equal or superior to described conjugates containing disulfide linkage. The new ligation method facilitates the synthesis of large quantities (>lo0 mg) of conjugates. INTRODUCTION Transferrin-polycation conjugates are efficient carriers for the uptake of DNA into eucariotic cells (I). This gene transfer technique, termed tramferrinfection, is based on receptor-mediated endocytosis of DNA complexed with polycation-transferrin conjugates (2,3). Our initial conjugate synthesis (1) involved the modification of one to two amino groups on the transferrin molecule with the bifunctional reagent succinimidyl34 2-pyridy1dithio)propionate (SPDP), followed by ligation to similarly modified polycations (polylysine or protamine) through the formation of disulfide bonds. Because there are more than 50 lysines on the large (about 80 kDa) transferrin protein, the actual site (or sites) of ligation to the polycation is unknown with this method. In this paper we describe the synthesis of new transferrin conjugates that are ligated with DNA-binding compounds in a specific manner through modification of the transferrin carbohydrate moiety. The conjugates thus obtained are free of any groups derived from chemical linking agents, since the connecting atoms are already present within the starting compounds. The carbohydrate group acts as anatural spacer that puts a 32-atom distance between the transferrin and the DNA binding moiety. This spacer effect may be important for appropriate presentation of the ligand to its receptor. As a DNA-binding compound, the polycation polylysine was used, similar to the use described in ref 1 or to the asialo-orosomucoid conjugates prepared by Wu and Wu (4). We have also prepared a novel type of transferrin conjugate that contains the DNA intercalator ethidium homodimer (5) as the DNAbinding group and demonstrate successful receptormediated gene delivery with these conjugates. EXPERIMENTAL PROCEDURES Human transferrin (iron-free), conalbumin (iron-free), and poly(L-lysine) were obtained from Sigma. Liquid chro- Abbreviations used: FITC, fluorescein ieothiocyenate; TfEtD, traneferrin-ethidium homodimer conjugate; TfpL, traneferrinpolytL- lysine) conjugate; HEPES, 4-(2-hydroxyethyl)-l-piperazineethanesulfonic acid