Hampton-Seabrook Estuary Habitat Restoration Compendium

The goal of this report is to identify restoration opportunities within the watershed derived from data on habitat change. Many other factors exist that are important in the identification and selection of restoration projects, including water quality and non-point source pollution, water withdrawal, harbor maintenance, recreational impacts, human history, and socioeconomic factors, among others. Although information regarding these factors is not explicitly included in this analysis, these factors must be considered and addressed as they may limit the potential for success in specific restoration effort

Hampton-Seabrook Estuary Restoration Compendium

The Hampton-Seabrook Estuary Habitat Restoration Compendium (HSEHRC) is a compilation of information on the historic and current distributions of salt marsh and sand dune habitats and diadromous fishes within the Hampton-Seabrook Estuary watershed. These habitats and species groups were selected due to the important ecological role they play within the watershed and with effective restoration and conservation efforts, will continue to play. Other ecologically important habitats and species, such as avifauna, shellfish and eelgrass beds, currently are or historically were present within the watershed. Shellfish and seagrass are recognized as important habitats within the Estuary, but were not included in the current report because a different analytical approach may be required for such dynamic and/or short-lived species. A recent report by the New Hampshire (NH) Audubon Society details modern bird use of the Hampton-Seabrook Estuary (McKinley and Hunt 2008). Restoration opportunities have been identified within the watershed by evaluating habitat loss and changes in land use over time. Restoration opportunities are not prioritized in order to allow the goals and objectives of each restoration practitioner to govern project selection. However, in accordance with an ecosystem-based approach to restoration, areas containing multi-habitat restoration opportunities are considered to be of the highest priority. Furthermore, restoration efforts should ensure processes critical for the support of restored components are maintained or reestablished. The goal of this report is to identify restoration opportunities within the watershed derived from data on habitat change. Many other factors exist that are important in the identification and selection of restoration projects, including water quality and non-point source pollution, water withdrawal, harbor maintenance, recreational impacts, human history, and socioeconomic factors, among others. Although information regarding these factors is not explicitly included in this analysis, these factors must be considered and addressed as they may limit the potential for success in specific restoration efforts We present a series of maps detailing changes in the extent of sand dune and salt marsh habitats over time, the current and historic distribution of seven diadromous fish species, and restoration opportunities within the Hampton-Seabrook Estuary and watershed. A narrative describes the methods used, the results of analyses and examples of prominent restoration projects. Each major section concludes with references used in the narrative and maps. The maps are available for viewing as portable document format (.pdf) files. For those with GIS capabilities, the ArcMap 9.2 project files, associated data files and metadata are included on the compact disc as well. The underlying concept and methods for the HSEHRC stem from a previous project conducted within the Great Bay Estuary, the Great Bay Estuary Restoration Compendium (Odell et al. 2006)

Great Bay Estuary Restoration Compendium

Single species approaches to natural resource conservation and management are now viewed as antiquated and oversimplified for dealing with complex systems. Scientists and managers who work in estuaries and other marine systems have urged adoption of ecosystem based approaches to management for nearly a decade, yet practitioners are still struggling to translate the ideas into practice. Similarly, ecological restoration projects in coastal systems have typically addressed one species or habitat. In recent years, efforts to focus on multiple species and habitats have increased. Our project developed an integrated ecosystem approach to identify multi-habitat restoration opportunities in the Great Bay estuary, New Hampshire. We created a conceptual site selection model based on a comparison of historic and modern distribution and abundance data, current environmental conditions, and expert review. Restoration targets included oysters and softshell clams, salt marshes, eelgrass beds, and seven diadromous fish species. Spatial data showing the historical and present day distributions for multiple species and habitats were compiled and integrated into a geographic information system. A matrix of habitat interactions was developed to identify potential for synergy and subsequent restoration efficiency. Output from the site selection models was considered within this framework to identify ecosystem restoration landscapes. The final products of these efforts include a series of maps detailing multi-habitat restoration opportunities extending from upland freshwater fish habitat down to the bay bottom. A companion guidance document was created to present project methods and a review of restoration methods. The authors hope that this work will help to stimulate and inform new restoration projects within the Great Bay estuarine system, and that it will serve as a foundation to be updated and improved as more information is collected

Censusing manatees: a report on the feasibility of using aerial surveys and mark and recapture techniques to conduct a population survey of the West Indian Manatee

This report results from an invitation to review the needs and prospects for capture-recapture and aerial census studies of the manatee (Trichechus manatus) in Florida. Three aerial reconnaissance flights provided a first hand view of manatee habitats, as follows: May 3, Suwannee River to Kings Bay and Crystal River (Rathbun, Eberhardt), May 4, Vero Beach to Ft. Lauderdale and Ft. Myers by way of Whitewater Bay (Rose, Percival, Eberhardt), and May 5, Cape Canaveral to Jacksonville, St. Johns River and Blue Spring (Rose, Kinnaird, Eberhardt). (24 page document

Blockchain-based Data Provenance for the Internet of Things

As more and more applications and services depend on data collected and provided by Internet of Things (IoT) devices, it is of importance that such data can be trusted. Data provenance solutions together with blockchain technology are one way to make data more trustworthy. However, current solutions do not address the heterogeneous nature of IoT applications and their data. In this work, we identify functional and non-functional requirements for a generic IoT data provenance framework, and conceptualise the framework as a layered architecture. Using a proof-of-concept implementation based on Ethereum smart contracts, data provenance can be realised for a wide range of IoT use cases. Benefits of a generic framework include simplified adoption and a more rapid implementation of data provenance for the IoT

Structural genomics analysis of uncharacterized protein families overrepresented in human gut bacteria identifies a novel glycoside hydrolase.

BackgroundBacteroides spp. form a significant part of our gut microbiome and are well known for optimized metabolism of diverse polysaccharides. Initial analysis of the archetypal Bacteroides thetaiotaomicron genome identified 172 glycosyl hydrolases and a large number of uncharacterized proteins associated with polysaccharide metabolism.ResultsBT_1012 from Bacteroides thetaiotaomicron VPI-5482 is a protein of unknown function and a member of a large protein family consisting entirely of uncharacterized proteins. Initial sequence analysis predicted that this protein has two domains, one on the N- and one on the C-terminal. A PSI-BLAST search found over 150 full length and over 90 half size homologs consisting only of the N-terminal domain. The experimentally determined three-dimensional structure of the BT_1012 protein confirms its two-domain architecture and structural analysis of both domains suggests their specific functions. The N-terminal domain is a putative catalytic domain with significant similarity to known glycoside hydrolases, the C-terminal domain has a beta-sandwich fold typically found in C-terminal domains of other glycosyl hydrolases, however these domains are typically involved in substrate binding. We describe the structure of the BT_1012 protein and discuss its sequence-structure relationship and their possible functional implications.ConclusionsStructural and sequence analyses of the BT_1012 protein identifies it as a glycosyl hydrolase, expanding an already impressive catalog of enzymes involved in polysaccharide metabolism in Bacteroides spp. Based on this we have renamed the Pfam families representing the two domains found in the BT_1012 protein, PF13204 and PF12904, as putative glycoside hydrolase and glycoside hydrolase-associated C-terminal domain respectively

Two Pfam protein families characterized by a crystal structure of protein lpg2210 from Legionella pneumophila.

BackgroundEvery genome contains a large number of uncharacterized proteins that may encode entirely novel biological systems. Many of these uncharacterized proteins fall into related sequence families. By applying sequence and structural analysis we hope to provide insight into novel biology.ResultsWe analyze a previously uncharacterized Pfam protein family called DUF4424 [Pfam:PF14415]. The recently solved three-dimensional structure of the protein lpg2210 from Legionella pneumophila provides the first structural information pertaining to this family. This protein additionally includes the first representative structure of another Pfam family called the YARHG domain [Pfam:PF13308]. The Pfam family DUF4424 adopts a 19-stranded beta-sandwich fold that shows similarity to the N-terminal domain of leukotriene A-4 hydrolase. The YARHG domain forms an all-helical domain at the C-terminus. Structure analysis allows us to recognize distant similarities between the DUF4424 domain and individual domains of M1 aminopeptidases and tricorn proteases, which form massive proteasome-like capsids in both archaea and bacteria.ConclusionsBased on our analyses we hypothesize that the DUF4424 domain may have a role in forming large, multi-component enzyme complexes. We suggest that the YARGH domain may play a role in binding a moiety in proximity with peptidoglycan, such as a hydrophobic outer membrane lipid or lipopolysaccharide

Impacto ambiental da cultura do arroz irrigado com uso de índice de qualidade de água (IQA).

O presente trabalho tem por objetivo avaliar o possível impacto da cultura do arroz irrigado sobre a qualidade das águas do Rio Camboriú (Camboriú/SC), através do estabelecimento de um índice de qualidade de água.bitstream/CNPMA/5829/1/comunicado_08.pd