DIAGNÓSTICOS MOLECULAR E CITOLÓGICO DE HERPESVIRUS FELINO TIPO 1, Chlamydophila felis E Mycoplasma felis, EM GATOS COM CONJUNTIVITE E/OU DOENÇA DO TRATO RESPIRATÓRIO SUPERIOR

A doença do trato respiratório superior (DTRS), apesar de muito comum em gatos, é frequentemente subvalorizada. Os sinais clínicos apresentados com mais frequência são espirros, tosse, secreção ocular e/ou nasal e conjuntivite. A conjuntivite felina apresenta, maioritariamente, uma natureza infeciosa, tendo como principais responsáveis o Herpesvírus felino-1, Chlamydophila felis e, possivelmente, Mycoplasma felis. Por vezes, o diagnóstico pode tornar-se desafiante, quer pela natureza dos agentes, quer pelos sinais clínicos inespecíficos. Os objetivos do presente trabalho foram: i) realizar o diagnóstico da infeção por estes agentes, através da citologia conjuntival e da Reação em Cadeia da Polimerase (PCR), em gatos com sinais clínicos de DTRS e/ou de conjuntivite e ii) avaliar a concordância entre os resultados do diagnóstico molecular e citológico. Para tal, numa população de 29 gatos com sinais clínicos de DTRS e/ou conjuntivite, foram realizadas duas recolhas de amostras conjuntivais, uma com zaragatoas e outra com escova de citologia, para os diagnósticos molecular e citológico, respetivamente. As citologias foram coradas pelo método de May Grunwald-Giemsa. Para cada amostra, procedeu-se a duas PCR convencionais, para amplificação do DNA de FHV-1 e M.felis e uma PCR em tempo real, para deteção de DNA de C. felis. Ao diagnóstico molecular, foi obtida uma frequência de infeção de 58,6% para FHV-1 e de 31,0% para C. felis. Não foi identificado nenhum gato com infeção por M. felis. No diagnóstico citológico conjuntival, apenas seis animais foram diagnosticados com C. felis, não tendo sido feita nenhuma observação suspeita de infeção para os restantes agentes. Os resultados evidenciaram uma elevada frequência de infeção por FHV-1 e C. felis. A PCR mostrou ser um meio de diagnóstico mais sensível para a deteção dos agentes em estudo, quando comparada com a citologia conjuntival. O presente estudo deixa em aberto a dúvida sobre o papel de M. felis enquanto agente etiológico primário na conjuntivite e DTRS

Crown gall caused by Agrobacterium tumefaciens species complex: a novel nursery disease of Tectona grandis in Brazil.

Made available in DSpace on 2019-04-25T00:44:08Z (GMT). No. of bitstreams: 1 Borges2018ArticleCrownGallCausedByAgrobacterium.pdf: 303222 bytes, checksum: 1be290babaac18e7abcd1ed3c612bdc5 (MD5) Previous issue date: 2018bitstream/item/196212/1/Borges2018-Article-CrownGallCausedByAgrobacterium.pd

A Paracoccidioides brasiliensis glycan shares serologic and functional properties with cryptococcal glucuronoxylomannan

The cell wall of the yeast form of the dimorphic fungus Paracoccidioides brasiliensis is enriched with alpha 1,3-glucans. in Cryptococcus neoformans, alpha 1,3-glucans interact with glucuronoxylomannan (GXM), a hetero-polysaccharide that is essential for fungal virulence. in this study, we investigated the occurrence of P. brasiliensis glycans sharing properties with cryptococcal GXM. Protein database searches in P. brasiliensis revealed the presence of sequences homologous to those coding for enzymes involved in the synthesis of GXM and capsular architecture in C. neoformans. in addition, monoclonal antibodies (mAbs) raised to cryptococcal GXM bound to P. brasiliensis cells. Using protocols that were previously established for extraction and analysis of C neoformans GXM, we recovered a P. brasiliensis glycan fraction composed of mannose and galactose, in addition to small amounts of glucose, xylose and rhamnose. in comparison with the C. neoformans GXM, the P. brasiliensis glycan fraction components had smaller molecular dimensions. the P. brasiliensis components, nevertheless, reacted with different GXM-binding mAbs. Extracellular vesicle fractions of P. brasiliensis also reacted with a GXM-binding mAb, suggesting that the polysaccharide-like molecule is exported to the extracellular space in secretory vesicles. An acapsular mutant of C. neoformans incorporated molecules from the P. brasiliensis extract onto the cell wall, resulting in the formation of surface networks that resembled the cryptococcal capsule. Coating the C. neoformans acapsular mutant with the P. brasiliensis glycan fraction resulted in protection against phagocytosis by murine macrophages. These results suggest that P. brasiliensis and C. neoformans share metabolic pathways required for the synthesis of similar polysaccharides and that P. brasiliensis yeast cell walls have molecules that mimic certain aspects of C. neoformans GXM. These findings are important because they provide additional evidence for the sharing of antigenically similar components across phylogenetically distant fungal species. Since GXM has been shown to be important for the pathogenesis of C neoformans and to elicit protective antibodies, the finding of similar molecules in P. brasiliensis raises the possibility that these glycans play similar functions in paracoccidiomycosis. (C) 2012 Elsevier Inc. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)NIHCenter for AIDS Research at EinsteinInterhemispheric Research Training Grant in Infectious Diseases, Fogarty International CenterDepartment of EnergyFiocruz MS, CDTS, BR-21040360 Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, BR-21941902 Rio de Janeiro, BrazilAlbert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10461 USAUniversidade Federal de São Paulo, Disciplina Biol Celular, BR-04023062 São Paulo, BrazilUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941903 Rio de Janeiro, BrazilAlbert Einstein Coll Med, Div Infect Dis, Dept Med, Bronx, NY 10461 USAUniversidade Federal de São Paulo, Disciplina Biol Celular, BR-04023062 São Paulo, BrazilNIH: AI033142NIH: AI033774NIH: AI052733NIH: HL059842Interhemispheric Research Training Grant in Infectious Diseases, Fogarty International Center: NIH D43-TW007129Department of Energy: DE-FG-9-93ER-20097Web of Scienc

Acute Conjunctivitis with Episcleritis and Anterior Uveitis Linked to Adiaspiromycosis and Freshwater Sponges, Amazon Region, Brazil, 2005

Medscape, LLC is pleased to provide online continuing medical education (CME) for this journal article, allowing clinicians the opportunity to earn CME credit. This activity has been planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education through the joint sponsorship of Medscape, LLC and Emerging Infectious Diseases. Medscape, LLC is accredited by the Accreditation Council for Continuing Medical Education (ACCME) to provide CME for physicians. Medscape, LLC designates this educational activity for a maximum of 0.5 AMA PRA Category 1 Credits™. Physicians should only claim credit commensurate with the extent of their participation in the activity. All other clinicians completing this activity will be issued a certificate of participation. To participate in this journal CME activity: (1) review the learning objectives and author disclosures; (2) study the education content; (3) take the post-test and/or complete the evaluation at http://www.medscape.com/cme/eidExternal Web Site Icon; (4) view/print certificate.\ud Learning Objectives\ud \ud Upon completion of this activity, participants will be able to:\ud \ud Describe the mechanism of infection for adiaspiromycosis.\ud Identify the age group most susceptible to ocular adiaspiromycosis.\ud Describe presenting symptoms associated with ocular adiaspiromycosis.\ud Describe the frequency of ocular lesions associated with adiaspiromycosis.\ud Identify risk factors for ocular adiaspiromycosis

Self-similar magnetoresistance of Fibonacci ultrathin magnetic films

We study numerically the magnetic properties (magnetization and magnetoresistance) of ultra-thin magnetic films (Fe/Cr) grown following the Fibonacci sequence. We use a phenomenological model which includes Zeeman, cubic anisotropy, bilinear and biquadratic exchange energies. Our physical parameters are based on experimental data recently reported, which contain biquadratic exchange coupling with magnitude comparable to the bilinear exchange coupling. When biquadratic exchange coupling is sufficiently large a striking self-similar pattern emerges.Comment: 5 pages, 5 EPS figures, REVTeX, accepted for publication in Phys. Rev.

An Artificial Intelligence application for drone-assisted 5G remote e-Health

Artificial intelligence (AI) algorithms are experiencing growing research interest due to their ability to improve decision making capabilities for promising applications in different economic sectors. The growing shift toward the Internet of Everything environments brought by devices embedded with sensors that can share information brings immense opportunity for new applications (apps). While these new apps thrive in resource-rich areas (i.e., capitals), neighboring cities that lack the resources and infrastructure to support them may be left behind. It is vital that new technologies can reach those who need them the most, especially healthcare-based. This article proposes an app-based approach for long-distance patient monitoring and care. The app would serve as a platform of communication between patients and healthcare staff, where the latter can send standardized video footage or pictures to the former (e.g., their primary care doctor). This feature is enhanced with a recurrent neural network algorithm as a validation tool for healthcare-related videos exchanged between patients and staff. Thus, the healthcare team does not need to check each video for validity, freeing their time for other activities

Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum-tomato 'Hawaii 7996' interactions.

Here, we carried out a multi‑algorithm stability analysis of eight candidate reference genes during interactions of ?Hawaii 7996? with one incompatible/avirulent and two compatible/virulent (= resistance‑breaking) bacterial isolates. Samples were taken at 24‑ and 96‑h post‑inoculation (HPI)

Desempenho reprodutivo de ovelhas das raças Morada Nova e Somalis Brasileira criadas na região Nordeste do Brasil.

Resumo: A eficiência dos sistemas de produção animal está relacionada a adequados índices reprodutivos, os quais são influenciados por fatores genéticos, ambientais e nutricionais. Em razão disso, objetivou-se com este estudo avaliar o desempenho reprodutivo de ovelhas da raça Morada Nova e Somalis Brasileira criadas na região Nordeste do Brasil. A estação de monta teve duração de 45 dias, onde foram utilizadas 156 ovelhas não gestantes, sendo 76 ovelhas pertencentes à raça Morada Nova e 80 a raça Somalis Brasileira. Os animais foram manejados em uma mesma área de caatinga raleada, parte dela enriquecida com capim-Massai, além de receberem concentrado no terço final da gestação. Os dados foram submetidos aos testes de Shapiro-Wilk e Bartlett, a fim de verificar os pressupostos de normalidade e homogeneidade de variância, respectivamente. Uma vez atendidos estes pressupostos, aplicou-se o teste F, através da Análise de Variância (ANOVA) e em seguida o teste t de Student. A fertilidade não diferiu estatisticamente em função do genótipo das ovelhas, porém verificou- se influência do grupo genético (P<0,05) para a taxa de prolificidade. Ovelhas da raça Morada Nova apresentaram maior prolificidade que a Somalis Brasileira. Não houve diferença estatística do grupo genético das ovelhas para o peso ao parto, entretanto fêmeas da raça Morada Nova apresentaram menor escore ao parto (P<0,05). Os resultados demonstraram que a prolificidade é diferente entre as raças, o que exige um manejo nutricional no terço final de gestação diferenciado para as fêmeas de maior prolificidade para evitar que haja redução no escore de condição corporal em função do maior percentual de partos gemelares. Abstract: The efficiency of animal production systems is linked to adequate reproductive rates, which are influenced by genetic, environmental and nutritional traits. For this reason, the aim of this work was to evaluate the reproductive performance of Morada Nova and Somalis Brasileira ewes raised in northeast Brazil. The breeding season lasted 45 days, where were used 156 non-pregnant sheep, being 76 Morada Nova and 80 Somalis Brasileira. The animals were handled in an area of thinned Caatinga enriched partially with Massai grass. All animais were fed with 400g of concentrated in the final third of pregnancy. Data were tested for normality and homogeneity by Shapiro-Wilk and Bartlett tests, respectively. After, we applied the F test, by analysis of variance (ANOVA) followed by the Student t test. The fertility did not differ statistically when compared both genotype of the sheep, but there was influence of genotype (P <0,05) for the rate of prolificacy. Morada Nova ewes showed higher prolificacy when compared to Somalis Brasileira ones. Both breeds showed similar weight at lambing, however females form Morada Nova genotype showed lower body condition score at lambing (P<0,05). The results showed different prolificacy between breeds, what requires an enhanced nutritional management during the final third of pregnancy to avoid losses in body condition score due multiple pregnancies

Métodos alternativos para aprendizado prático dos conteúdos da disciplina técnica cirúrgica veterinária

O artigo não apresenta resumo