18 research outputs found
Macrophage and tumor cell responses to repetitive pulsed X-ray radiation
To study a response of tumor cells and macrophages to the repetitive pulsed low-dose X-ray radiation. Methods. Tumor growth and lung metastasis of mice with an injected Lewis lung carcinoma were analysed, using C57Bl6. Monocytes were isolated from a human blood, using CD14+ magnetic beads. IL6, IL1-betta, and TNF-alpha were determined by ELISA. For macrophage phenotyping, a confocal microscopy was applied. "Sinus-150" was used for the generation of pulsed X-ray radiation (the absorbed dose was below 0.1 Gy, the pulse repetition frequency was 10 pulse/sec). The irradiation of mice by 0.1 Gy pulsed X-rays significantly inhibited the growth of primary tumor and reduced the number of metastatic colonies in the lung. Furthermore, the changes in macrophage phenotype and cytokine secretion were observed after repetitive pulsed X-ray radiation. Conclusion. Macrophages and tumor cells had a different response to a low-dose pulsed X-ray radiation. An activation of the immune system through changes of a macrophage phenotype can result in a significant antitumor effect of the low-dose repetitive pulsed X-ray radiation
C-fos expression and cell proliferation in mice after exposure to nanosecond repetitively-pulsed X-rays
Π ΡΡΠ°ΡΡΠ΅ ΡΠΊΠ°Π·Π°Π½ΠΎ Rostov, Vladimir V
Π Π°Π·Π»ΠΈΡΠΈΡ ΡΡΠ΅ΠΊΡΠΎΠ² ΠΈΠΌΠΏΡΠ»ΡΡΠ½ΠΎ-ΠΏΠ΅ΡΠΈΠΎΠ΄ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ²ΡΠΊΠΎΠ³ΠΎ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΡ Π² ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΊΠ°Ρ Π»ΠΈΠ½ΠΈΠΈ MOLT-4 ΠΈ Π»ΠΈΠΌΡΠΎΡΠΈΡΠ°Ρ ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
Effects of ionizing radiation registered in cells after low dose irradiation are still poorly understood. Thus, the aim of this study was to analyze effects of pulsed X-rays on level of radiation-induced DNA double-strand breaks and their repair kinetics in cancer and normalhuman cells in vitro. Analysis of radiation-induced Ξ³H2AX and 53BP1 repair foci in MOLT-4 cells with lymphoblastic origin was used for assessment of DNA double-strand breaks (DSB) in these cells. Number of residual radiation-induced Ξ³H2AX and 53BP1 foci at 18 hafter irradiation depended on frequency of X-ray pulses: at 8 pulses per second effect was highest in MOLT-4 cells and lowest in peripheral blood lymphocytes. It suggests that pulsed X-rays with various frequencies could be used for target influence on cancer cells being lessdeleterious for normal human cells.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Ρ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΈ, ΡΠΏΠΎΡΠΎΠ±Π½ΡΠ΅ Π³Π΅Π½Π΅ΡΠΈΡΠΎΠ²Π°ΡΡ ΠΈΠΌΠΏΡΠ»ΡΡΠ½ΠΎ-ΠΏΠ΅ΡΠΈΠΎΠ΄ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ²ΡΠΊΠΎΠ΅ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΠ΅ (ΠΠΠ Π) Π² Π½Π°Π½ΠΎΡΠ΅ΠΊΡΠ½Π΄Π½ΠΎΠΌ Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠ°ΡΡΠΎΡΡ ΠΏΠΎΠ²ΡΠΎΡΠ΅Π½ΠΈΡ ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ² ΠΈ Π΄ΠΎΠ·Ρ Π·Π° ΠΈΠΌΠΏΡΠ»ΡΡ. Π¦Π΅Π»ΡΡ Π½Π°ΡΡΠΎΡΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ°Π» Π°Π½Π°Π»ΠΈΠ· Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΠΠ Π Π½Π° ΡΡΠΎΠ²Π΅Π½Ρ Π΄Π²ΡΠ½ΠΈΡΠ΅Π²ΡΡ
ΡΠ°Π·ΡΡΠ²ΠΎΠ² ΠΠΠ Π² ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΈ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΊΠ°Ρ
ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
in vitro. ΠΠ»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΡΠΎΠ²Π½Ρ Π΄Π²ΡΠ½ΠΈΡΠ΅Π²ΡΡ
ΡΠ°Π·ΡΡΠ²ΠΎΠ² ΠΠΠ ΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΡ
ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΡΡ ΠΌΠ΅ΡΠΎΠ΄ Π°Π½Π°Π»ΠΈΠ·Π° ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΡΡ
ΡΠΎΠΊΡΡΠΎΠ² Π±Π΅Π»ΠΊΠΎΠ² ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΈ ΠΠΠ Ξ³H2AX ΠΈ 53BP1 Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ Π»ΠΈΠ½ΠΈΠΈ MOLT-4, ΠΈΠΌΠ΅ΡΡΠ΅ΠΉ Π»ΠΈΠΌΡΠΎΠ±Π»Π°ΡΡΠ½ΠΎΠ΅ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΠ΅, ΠΏΠΎΡΠ»Π΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΠΠ Π Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
in vitro. ΠΠ°ΠΈΠ±ΠΎΠ»ΡΡΠΈΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΎΠΊΡΡΠΎΠ² Ξ³H2AX ΠΈ 53BP1 ΡΠ΅ΡΠ΅Π· 18 Ρ ΠΏΠΎΡΠ»Π΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ, ΡΠ²Π»ΡΡΡΠΈΠΉΡΡ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠΌ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΈ ΠΠΠ, Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
Π»ΠΈΠ½ΠΈΠΈ MOLT-4 ΠΎΡΠΌΠ΅ΡΠ°Π»ΡΡ ΠΏΡΠΈ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠΈ ΠΠΠ Π Ρ ΡΠ°ΡΡΠΎΡΠΎΠΉ ΠΏΠΎΠ²ΡΠΎΡΠ΅Π½ΠΈΡ ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ² 8 ΠΈΠΌΠΏ./Ρ, ΠΏΡΠΈ ΠΊΠΎΡΠΎΡΠΎΠΉ ΡΠ°Π½Π΅Π΅ Π² Π»ΠΈΠΌΡΠΎΡΠΈΡΠ°Ρ
Π½Π°Π±Π»ΡΠ΄Π°Π»ΠΎΡΡ Π½Π°ΠΈΠΌΠ΅Π½ΡΡΠ΅Π΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ Π΄Π²ΡΠ½ΠΈΡΠ΅Π²ΡΡ
ΡΠ°Π·ΡΡΠ²ΠΎΠ² ΠΠΠ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠΊΠ°Π·ΡΠ²Π°ΡΡ, ΡΡΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠΠ Π Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΡΠ°ΡΡΠΎΡΠ°ΠΌΠΈ ΠΏΠΎΠ²ΡΠΎΡΠ΅Π½ΠΈΡ ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ² ΠΌΠΎΠΆΠ΅Ρ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡΡΠ΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΎΠ²Π°ΡΡ Π½Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ, Π½Π΅Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π°Ρ ΠΏΡΠΈ ΡΡΠΎΠΌ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
ΠΠ΅ΠΉΡΡΠ²ΠΈΠ΅ Π½Π°Π½ΠΎΡΠ΅ΠΊΡΠ½Π΄Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΏΡΠ»ΡΡΠ½ΠΎ-ΠΏΠ΅ΡΠΈΠΎΠ΄ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΠ²ΠΎΠ»Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΡ Π½Π° ΠΏΡΠΎΡΠ΅ΡΡΡ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ
The effects of pulse periodic microwaves (10 GHz, duration of pulse 100 ns, pulse repetition frequency 4β19 pps, peak power density 40β1 520 W/cm2 ) on the reparative regeneration of full-thickness skin wounds on mice was investigated. This effect depends on the pulse repetition frequency and peak power density.ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΠΈΠΌΠΏΡΠ»ΡΡΠ½ΠΎ-ΠΏΠ΅ΡΠΈΠΎΠ΄ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΠ²ΠΎΠ»Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΡ (10 ΠΠΡ, Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ² 100 Π½Ρ, ΡΠ°ΡΡΠΎΡΠ° ΠΏΠΎΠ²ΡΠΎΡΠ΅Π½ΠΈΡ 4β19 ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ² Π² ΡΠ΅ΠΊΡΠ½Π΄Ρ, ΠΏΠΈΠΊΠΎΠ²Π°Ρ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΡ ΠΏΠΎΡΠΎΠΊΠ° ΠΌΠΎΡΠ½ΠΎΡΡΠΈ 40β1 520 ΠΡ/ΡΠΌ2 ) Π½Π° ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠ²Π½ΡΡ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΡ ΠΏΠΎΠ»Π½ΠΎΡΠ»ΠΎΠΉΠ½ΠΎΠΉ ΠΊΠΎΠΆΠ½ΠΎΠΉ ΡΠ°Π½Ρ Ρ ΠΌΡΡΠ΅ΠΉ. ΠΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠ΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΠΌΠΎΠΆΠ΅Ρ ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°ΡΡ Π·Π°ΠΆΠΈΠ²Π»Π΅Π½ΠΈΠ΅ ΡΠ°Π½. ΠΠ°Π½Π½ΡΠΉ ΡΡΡΠ΅ΠΊΡ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΡΠ°ΡΡΠΎΡΡ ΠΏΠΎΠ²ΡΠΎΡΠ΅Π½ΠΈΡ ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ² ΠΈ Π²Π΅Π»ΠΈΡΠΈΠ½Ρ ΠΏΠΈΠΊΠΎΠ²ΠΎΠΉ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ ΠΏΠΎΡΠΎΠΊΠ° ΠΌΠΎΡΠ½ΠΎΡΡΠΈ
ΠΡΠ΅Π½ΠΊΠ° Π²Π»ΠΈΡΠ½ΠΈΡ Π½Π°Π½ΠΎΡΠ΅ΠΊΡΠ½Π΄Π½ΡΡ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ²ΡΠΊΠΈΡ ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ² Π½Π° ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΌΠΈΡΠΎΡ ΠΎΠ½Π΄ΡΠΈΠΉ ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΌΡΡΠ΅ΠΉ
The effect of nanosecond pulses of X-ray (pulse repetition rate of 8β22 pulses per second, dose 0,3β1,8 mR per pulse) on the functional activity of isolated mitochondria of mice liver. The effects of changing the rate of oxygen consumption by mitochondria in different metabolic states of Chance and the degree of coupling of oxidation and phosphorylation were investigated. That effect depends on the parameters of exposure.ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ²ΡΠΊΠΈΡ
ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ² Π½Π°Π½ΠΎΡΠ΅ΠΊΡΠ½Π΄Π½ΠΎΠΉ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ (ΡΠ°ΡΡΠΎΡΠ° ΠΏΠΎΠ²ΡΠΎΡΠ΅Π½ΠΈΡ 8β22 ΠΈΠΌΠΏΡΠ»ΡΡΠ° Π² ΡΠ΅ΠΊΡΠ½Π΄Ρ, Π΄ΠΎΠ·Π° Π² ΠΈΠΌΠΏΡΠ»ΡΡΠ΅ 0,3β1,8 ΠΌΠ ) Π½Π° ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΉ ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΌΡΡΠ΅ΠΉ. ΠΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠ΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½ΡΠ΅Ρ ΡΠΊΠΎΡΠΎΡΡΡ ΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΡ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄Π° ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΡΠΌΠΈ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΎΡΡΠΎΡΠ½ΠΈΡΡ
ΠΏΠΎ Π§Π°Π½ΡΡ ΠΈ ΡΡΠ΅ΠΏΠ΅Π½Ρ ΡΠΎΠΏΡΡΠΆΠ΅Π½ΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΠΎΠΊΠΈΡΠ»Π΅Π½ΠΈΡ ΠΈ ΡΠΎΡΡΠΎΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΠ°Π½Π½ΡΠΉ ΡΡΡΠ΅ΠΊΡ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ
Diferentefects of pulsed X-rays in MOLT-4 cel line and human peripheral blod lymphocytes
Effects of ionizing radiation registered in cells after low dose irradiation are still poorly understood. Thus, the aim of this study was to analyze effects of pulsed X-rays on level of radiation-induced DNA double-strand breaks and their repair kinetics in cancer and normalhuman cells in vitro. Analysis of radiation-induced Ξ³H2AX and 53BP1 repair foci in MOLT-4 cells with lymphoblastic origin was used for assessment of DNA double-strand breaks (DSB) in these cells. Number of residual radiation-induced Ξ³H2AX and 53BP1 foci at 18 hafter irradiation depended on frequency of X-ray pulses: at 8 pulses per second effect was highest in MOLT-4 cells and lowest in peripheral blood lymphocytes. It suggests that pulsed X-rays with various frequencies could be used for target influence on cancer cells being lessdeleterious for normal human cells
The effects of nanosecond X-ray pulses on functional activity
The effect of nanosecond pulses of X-ray (pulse repetition rate of 8β22 pulses per second, dose 0,3β1,8 mR per pulse) on the functional activity of isolated mitochondria of mice liver. The effects of changing the rate of oxygen consumption by mitochondria in different metabolic states of Chance and the degree of coupling of oxidation and phosphorylation were investigated. That effect depends on the parameters of exposure
The influence of nanosecond microwave pulses on the regeneration processes
The effects of pulse periodic microwaves (10 GHz, duration of pulse 100 ns, pulse repetition frequency 4β19 pps, peak power density 40β1 520 W/cm2 ) on the reparative regeneration of full-thickness skin wounds on mice was investigated. This effect depends on the pulse repetition frequency and peak power density