Spatial and Temporal Distribution of Fungicides Applied to Creeping Bentgrass

Turf managers often rely on fungicides to limit damage caused by root diseases. Since fungicides do not move basipetally, they are effective only when fungitoxic concentrations are delivered to the rhizosphere. This research focused on the distribution of modern fungicides in verdure, thatch, sand, and roots of creeping bentgrass (Agrostis stolonifera L. var. palustris (Huds.) Farw.) maintained as a putting green. Fungicides (azoxystrobin (methyl (E)-2-[2-[6-(2-cyanophenoxy)pyrimidin-4-yloxy]phenyl]-3-methoxyacrylate), propiconazole (1,2,4-Triazole, 1-((2-(2,4-dichlorophenyl)-4-propyl-1,3-dioxolan-2-yl)methyl), pyraclostrobin (carbamic acid, [2-[[[1-(4-chlorophenyl)-1H-pyrazol-3-yl]oxy]methyl]phenyl]methoxy-,methyl ester), and thiophanate-methyl (dimethyl 4,4\u27-o-phenylenebis[3-thioallophanate]) were applied to replicate field plots in a water volume of 815 L ha-1. Plots were sampled over time (0, 3, 7, 10, 14, 17, 21 days after application) by extracting cores measuring 2 cm diameter by 3.8 cm deep. Cores were separated into verdure/thatch, sand, and roots before quantitative determination (liquid chromatography, triple quadrupole mass spectrometry) of fungicide residues. Fungicide residues in verdure/thatch declined steadily with time and support previously reported results describing fungicide depletion. Fungicides were detected in roots and sand within 5 hours of application, although at very low (1-15 ppm) concentrations. Residues in roots and sand remained at low levels throughout the experiment. Fungicides differed with respect to amounts recovered per turfgrass component

Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses

Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis poae and Ophiosphaerella korrae, “Detection of root-infecting fungi on cool-season turfgrasses using loop-mediated isothermal amplification and recombinase polymerase amplification” (Karakkat et al., 2018) [1]. The data provided here describe the information for designing primers and probes for LAMP and RPA, how specific they are for each of the three fungi, and the evaluation of RPA on field samples. Keywords: Turfgrass, LAMP, RPA, Gaeumannomyces avenae, Ophiosphaerella korrae, Magnaporthiopsis poa