Comparison of genes either similarly or differentially expressed.

<p>(A) Entire gene set divided into 4 quantiles based on their level of expression to enable determination percentage of similarly expressed genes and differentially expressed genes. (B) Coding gene set divided into 4 quantiles based on their level of expression to enable determination percentage of similarly expressed genes and differentially expressed genes.</p

Comparative drug response profile between IBC PDX Bcx017 mouse model and Bcx017 PDXEx tissue model.

<p>A) Actively growing (~200–350 mm3) implants in mice were grouped and treated with the indicated drugs for 21days. The graph summarizes data from a previously published in vivo study [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0195932#pone.0195932.ref069" target="_blank">69</a>]. B) Tumor cells tagged with a nanoparticle assembly of iron oxide and iron nanoparticles cross-linked with poly-L-lysine (NanoshuttleTM) were bio-printed into ultra low attachment plates. The PDXEx bio-print was treated with the indicated drugs at the indicated dose range for 5 days.</p

Additional file 3: of Selinexor (KPT-330) demonstrates anti-tumor efficacy in preclinical models of triple-negative breast cancer

Effects of selinexor on the cell cycle. SUM-159PT and MDA-MB-468 cells were treated with 1 nM paclitaxel alone (2 nM for MDA-MB-468), 400 nM selinexor alone, and the combination of both. After 72 hours, cells were stained with propidium iodide and analyzed via flow cytometry. (DOCX 514 kb

Lead candidates identified from a high throughput drug screen utilizing the PDXEx screening platform.

<p>A high throughput small molecule screen was performed utilizing the Bcx087 triple negative PDXEx screening platform and the small molecule Anti-Cancer 386 Compound Library (Selleckchem). The ex vivo tumor tissue array was incubate with the drugs at 37oC for 5 days. The top hits were identified as agents having a superior anticancer effect to that of docetaxel and doxorubicin at 250nM.</p

Gene expression levels of the top 50 ranked genes.

<p>Gene expression levels of the top 50 ranked genes.</p

Generation of preclinical PDX-derived ex-vivo model.

<p>(A) A freshly harvested PDX tumor from a mouse was finely chopped to release all its cellular content. The released cells are filtered to separated them form fat and necrotic tissue and tagged with a nanoparticle assembly of iron oxide and iron nanoparticles cross-linked with poly-L-lysine (NanoshuttleTM) by an overnight incubation prior to been placed under a magnetic field. (Bio-AssemblerTM) (n3D Biosciences Inc.). (B) The levitating mass of cells developed into a loose unstructured mass by day 2 of incubation and (C) into a more structured compact mass by day 4 of incubation. (D) Immunohistochemistry analysis of PDX tissue and PDXEx tissue revealed a similar tissue architecture and staining for E-cadherin, Vimentin, Ki67 and pSMAD2.</p

Layout of biological pathways common to all 3 PDX tumors.

<p>The Top 25 out of 1116 pathways are presented.</p

Tissue characteristics of the ex-vivo tumor model.

<p>(A) Bio-printing cells–Tumor cells tagged with a nanoparticle assembly of iron oxide and iron nanoparticles cross-linked with poly-L-lysine (NanoshuttleTM) were dispensed into an ultralow attachment 96 well tissue culture plate placed on a 96 well magnetic drive (n3D Biosciences, Inc). (B) Morphology (4x magnification) of the ex-vivo tumor tissue prints after 5 days of growth at 37°C revealing uniformly sized structures. (C) Proliferative capacity of the PDXEx tissue following 5 days of culture at 37°C.</p