Isoflurane Preconditioning at Clinically Relevant Doses Induce Protective Effects of Heme Oxygenase-1 on Hepatic Ischemia Reperfusion in Rats

<p>Abstract</p> <p>Background</p> <p>Activation of heme oxygenase-1 (HO-1) has been proved to reduce damages to the liver in ischemia reperfusion injury. The objective of present study was to determine whether clinic relevant doses of isoflurane treatment could be sufficient to activate HO-1 inducing, which confers protective effect against hepatic ischemia-reperfusion injury.</p> <p>Methods</p> <p>The hepatic artery and portal vein to the left and the median liver lobes of forty male Sprague-Dawley rats were occluded for 60 minutes. Reperfusion was allowed for 4 hours before the animal subjects were sacrificed. Six groups (n = 12) were included in the study. A negative control group received sham operation and positive control group a standard ischemia-reperfusion regimen. The third group was pretreated with isoflurane prior to the ischemia-reperfusion. The fourth group received an HO-1 inhibitor zinc protoporphyrin (Znpp) prior to the isoflurane pretreatment and the ischemia-reperfusion. The fifth group received Znpp alone before ischemia-reperfusion procedure, and the sixth group was administrated with a HO-1 inducer hemin prior to IR. HO-1 in the liver was measured using an enzymatic activity assay, a Western blot analysis, as well as immunohistochemical method. Extent of liver damage was estimated by determination of the serum transaminases, liver lipid peroxidation and hepatic histology. Infiltration of the liver by neutrophils was measured using a myeloperoxidase activity assay. TNFα mRNA in the liver was measured using RT-PCR.</p> <p>Results</p> <p>Isoflurane pretreatment significantly attenuated the hepatic injuries and inflammatory responses caused by the ischemia reperfusion. Selectively inhibiting HO-1 with ZnPP completed blocked the protective effects of isoflurane. Inducing HO-1 with hemin alone produced protective effects similar in magnitude to that of isoflurane.</p> <p>Conclusions</p> <p>Clinic relevant doses of isoflurane attenuate ischemia reperfusion injury in rats by increasing the HO-1 expression and activity.</p

Bayesian panel smooth transition model with spatial correlation.

In this paper, we propose a spatial lag panel smoothing transition regression (SLPSTR) model ty considering spatial correlation of dependent variable in panel smooth transition regression model. This model combines advantages of both smooth transition model and spatial econometric model and can be used to deal with panel data with wide range of heterogeneity and cross-section correlation simultaneously. We also propose a Bayesian estimation approach in which the Metropolis-Hastings algorithm and the method of Gibbs are used for sampling design for SLPSTR model. A simulation study and a real data study are conducted to investigate the performance of the proposed model and the Bayesian estimation approach in practice. The results indicate that our theoretical method is applicable to spatial data with a wide range of spatial structures under finite sample

The Association and Predictive Value of Lipid Ratios to Metabolic Syndrome: a Multistage Cross-sectional Study

Background With the growing incidence of metabolic diseases, there has been increasing attention given to the prevention and control of metabolic syndrome (MS) . As important screening markers of MS, the relationship between lipid ratios and MS has become a hot research topic. Objective To analyse the association and predictive value of triglyceride/high-density lipoprotein cholesterol (TG/HDL-C) ratio, total cholesterol/HDL-C (TC/HDL-C) ratio, low-density lipoprotein cholesterol/ HDL-C (LDL-C/HDL-C) ratio and non-HDL-C to the risk of MS in populations included in a multistage cross-sectional study in Guizhou Province. Methods This study retrospectively selected natural populations (totally 21 727 cases) from Guizhou who attended the National Survey of Chronic Diseases and Risk Factors in Surveillance Regions 2010, China Chronic Disease and Risk Factor Surveillance 2013, and two waves of China Adult Chronic Disease and Nutrition Surveillance (2015 and 2018) . Baseline data were collected, and according to MS prevalence included in which, subjects were divided into MS group (n=4 981) and non-MS group (n=16 746) . The receiver operating characteristic (ROC) curve analysis was used to evaluate the predictive value of TG/HDL-C ratio, TC/HDL-C ratio, LDL-C/HDL-C ratio and non-HDL-C for MS in males and females, respectively. The Delong test was used to compare the values of area under the ROC curve (AUC) of lipid ratios in predicting MS. Multivariate Logistic regression was used to analyze the association of lipid ratios with MS measured using odds ratio (OR) with 95% confidence interval (CI) , and to identify factors associated with MS among subjects stratified by survey time, age, sex, body mass index (BMI) , smoking and alcohol consumption. Results There were statistically significant differences in mean age, sex ratio, distribution of ethnicities, education level and marital status, prevalence of smoking and alcohol consumption, and mean BMI, TG/HDL-C ratio, TC/HDL-C ratio, LDL-C/HDL-C ratio and non-HDL-C between MS group and non-MS group (P&lt;0.05) . TG/HDL-C ratio had a higher AUC value than TC/HDL-C ratio (Z=17.822, P&lt;0.001) , LDL-C/HDL-C ratio (Z=23.813, P&lt;0.001) and non-HDL-C (Z=27.608, P&lt;0.001) . The value of AUC of TG/HDL-C ratio was higher in males than that in females (Z=4.299, P&lt;0.001) . And the value of AUC of LDL-C/HDL-C ratio in males was lower than that in females (Z=2.061, P=0.039) . Multivariate Logistic regression analysis showed that in &lt;60 years old, ≥60 years old, male, female, BMI&lt;24.0 kg/m2, BMI≥24.0 kg/m2, smoking, non-smoking, drinking and non-drinking groups, TG/HDL-C ratio, TC/HDL-C ratio, LDL-C/HDL-C ratio and non-HDL-C were associated with the prevalence of MS (P&lt;0.05) . Conclusion TG/HDL-C ratio has a good predictive value for MS. TG/HDL-C ratio, TC/HDL-C ratio, LDL-C/HDL-C ratio and non-HDL-C are associated factors of MS, which should be paid more attention clinically in females, individuals with BMI&lt;24.0 kg/m2, non-smokers or non-drinkers

Effect of perioperative high-dose transdermal nicotine patch on pain sensitivity among male abstinent tobacco smokers undergoing abdominal surgery: a randomized controlled pilot study

Background and AimsPrevious studies have focused on the role of perioperative nicotine replacement therapy (NRT) in improving the success rate of long-term smoking cessation in tobacco smokers. This study aimed to measure the effectiveness of high-dose NRT in alleviating postoperative pain for male abstinent smokers receiving abdominal surgery. DesignThis was a parallel-group, randomized, double-blind and controlled pilot trial. Setting and participantsIn total, 101 male smoking-abstinent patients from the Eastern Hepatobiliary Surgery Hospital, Shanghai, China, from 8 October 2018 to 10 December 2021. InterventionsPatients started smoking cessation on admission to the hospital ward. Patients received 24-hour transdermal nicotine patches (n = 50) or placebo (n = 51) every day from admission until 48 hours after surgery. MeasurementsThe primary outcomes were pre-surgery pain thresholds and total consumption of analgesics within the first 48 hours after surgery. Secondary outcomes included postoperative pain and sedation scores, nausea, vomiting and fever frequency within the treatment period. FindingsBoth pre-surgery electrical and mechanical pain thresholds in the NRT group were higher than those in the placebo group (P = 0.004 and P = 0.020, respectively). The 48-hour postoperative analgesic consumption was significantly lower for smoking-abstinent patients receiving NRT than those receiving placebo (standardized morphine equivalent requirement, median [interquartile range], 1.80 [1.47, 2.32] mg/kg vs 2.22 [1.62, 2.82] mg/kg, P = 0.011). Postoperative pain intensity was significantly lower in the NRT group than that in the placebo group at 1st hour and 24th hour post-surgery (P &lt; 0.001 and P = 0.012, respectively). The incidence of treatment-related adverse events was not significantly different between groups. ConclusionsPerioperative high-dose nicotine replacement therapy may help to relieve postoperative pain among male smoking-abstinent patients undergoing abdominal surgery.</p

Comparative genome analysis of Sesbania cannabina-nodulating Rhizobium spp. revealing the symbiotic and transferrable characteristics of symbiosis plasmids

Symbiotic nitrogen fixation between legumes and rhizobia makes a great contribution to the terrestrial ecosystem. The suc-cessful symbiosis between the partners mainly depends on the nod and nif genes in rhizobia, while the specific symbiosis is mainly determined by the structure of Nod factors and the corresponding secretion systems (type III secretion system; T3SS), etc. These symbiosis genes are usually located on symbiotic plasmids or a chromosomal symbiotic island, both could be transferred interspecies. In our previous studies, Sesbania cannabina- nodulating rhizobia across the world were classified into 16 species of four genera and all the strains, especially those of Rhizobium spp., harboured extraordinarily highly conserved symbiosis genes, suggesting that horizontal transfer of symbiosis genes might have happened among them. In order to learn the genomic basis of diversification of rhizobia under the selection of host specificity, we performed this study to compare the complete genome sequences of four Rhizobium strains associated with S. cannabina, YTUBH007, YTUZZ027, YTUHZ044 and YTUHZ045. Their complete genomes were sequenced and assembled at the replicon level. Each strain represents a different species according to the average nucleotide identity (ANI) values calculated using the whole-genome sequences; furthermore, except for YTUBH007, which was classified as Rhizobium binae, the remaining three strains were identified as new candidate species. A single symbiotic plasmid sized 345-402 kb containing complete nod, nif, fix, T3SS and conjugal transfer genes was detected in each strain. The high ANI and amino acid identity (AAI) values, as well as the close phylogenetic relationships among the entire symbiotic plasmid sequences, indicate that they have the same origin and the entire plasmid has been transferred among different Rhizobium species. These results indicate that S. cannabina stringently selects a certain symbiosis gene back-ground of the rhizobia for nodulation, which might have forced the symbiosis genes to transfer from some introduced rhizobia to the related native or local- condition-adapted bacteria. The existence of almost complete conjugal transfer related elements, but not the gene virD, indicated that the self-transfer of the symbiotic plasmid in these rhizobial strains may be realized via a virD- independent pathway or through another unidentified gene. This study provides insight for the better understanding of high-frequency symbiotic plasmid transfer, host-specific nodulation and the host shift for rhizobia

Effect of Cr Content on the Microstructure of Casting Infiltration Layers: Simulations and Experiments

High chromium cast irons are commonly used as casting infiltration layers in the applications of wear resistance. The formation mechanism of the casting infiltration layer is essential to better develop the surface wear resistance materials using the casting infiltration method. In the present work, casting infiltration layers with various Cr contents were fabricated in situ on the surface of parent ZG45 steel. CALPHAD-type calculations using Thermo-Calc software, SEM, EDS and microhardness tests were performed to study the effect of Cr on the microstructure and hardness of casting infiltration layers. All the microstructures of casting infiltration layers were composed of pearlite matrix and eutectic M7C3 carbide. With the increase in Cr content from 7.01 wt.% to 17.20 wt.%, the amount of M7C3 carbide increased from 5.05 vol.% to 13.12 vol.%, resulting in the increment of microhardness. With the aid of simulations, the solidification behavior and formation mechanism of casting infiltration layers were revealed

Effect of Cr Content on the Microstructure of Casting Infiltration Layers: Simulations and Experiments

High chromium cast irons are commonly used as casting infiltration layers in the applications of wear resistance. The formation mechanism of the casting infiltration layer is essential to better develop the surface wear resistance materials using the casting infiltration method. In the present work, casting infiltration layers with various Cr contents were fabricated in situ on the surface of parent ZG45 steel. CALPHAD-type calculations using Thermo-Calc software, SEM, EDS and microhardness tests were performed to study the effect of Cr on the microstructure and hardness of casting infiltration layers. All the microstructures of casting infiltration layers were composed of pearlite matrix and eutectic M7C3 carbide. With the increase in Cr content from 7.01 wt.% to 17.20 wt.%, the amount of M7C3 carbide increased from 5.05 vol.% to 13.12 vol.%, resulting in the increment of microhardness. With the aid of simulations, the solidification behavior and formation mechanism of casting infiltration layers were revealed

抑郁症与2型糖尿病风险及其社会人口学因素的关联:中国西南贵州省的一项前瞻性队列研究

Abstract Background Depression may be associated with the risk of developing type 2 diabetes. The goal of this study was to explore the association of severe of depression with the risk of type 2 diabetes in adults in Guizhou, China. Methods A 10‐year prospective cohort study of 7158 nondiabetes adults aged 18 years or older was conducted in Guizhou, southwest China from 2010 to 2020. The Patient Health Questionnaire‐9 (PHQ‐9) was used to measure the prevalence of depression. Cox proportional hazard models were used to estimated hazard ratios (HRs) and 95% confidence intervals (95% CIs) of depression and incident type 2 diabetes. A quantile regression (QR) analytical approach were applied to evaluate the associations of PHQ‐9 score with plasma glucose values. Results A total of 739 type 2 diabetes cases were identified during a median follow‐up of 6.59 years. The HR (95% CI) per 1‐SD increase for baseline PHQ‐9 score was 1.051 (1.021, 1.082) after multivariable adjustment. Compared with participants without depression, those with mild or more advanced depression had a higher risk of incident type 2 diabetes (HR:1.440 [95% CI, 1.095, 1.894]). Associations between depression with type 2 diabetes were suggested to be even stronger among women or participants aged ≥45 years (p < .05). There are significant positive associations of PHQ‐9 score with 2‐h oral glucose tolerance test blood glucose levels. Conclusion Depression significantly increased the risk of incident type 2 diabetes, especially in women, participants aged ≥45 years, Han ethnicity, and urban residents. These findings highlighted the importance and urgency of depression health care