2 research outputs found
The protective effects of omega-3 fatty acids on rat testicular tissue
Objectives: In this study, the protective effect of omega-3 fatty acids on testicular tissue was aimed to investigate at biochemical levels.Materials and methods: Totally, 16 adult male Wistar rats were divided into two groups. Rats in Group I were used as control and only saline was given by intragastric gavage. Rats in Group II, 400 mg/kg dose ω-3 fatty acids were given daily by intragastric gavage. At the end of the six-week experimental period, all rats were killed by decapitation. The testicular tissue specimens taken from animals, superoxide dismutase, glutathione peroxidase, malondialdehyde, enzyme activities were measured spectrophotometrically. In addition, blood testosterone levels were examined.Results: In our study, ω-3 fatty acids in rats were given a statistically significant increase in the levels of superoxide dismutase, and glutathione peroxidase a statistically significant decrease in malondialdehyde levels were determined when compared to control group. In addition, ω-3 fatty acids in rats given a statistically significant increase in blood testosterone levels were observed.Conclusion: We concluded that ω-3 fatty acid had favorable effects in rat testis tissue by preventing oxidative damage and increasing the level of testosterone
Sıçanlarda Formaldehit Maruziyetiyle Testislerde Oluşan Morfolojik Değişiklikler Üzerine Melatonin Hormonunun Koruyucu Etkisi
Çalışmamızda, formaldehitin testis dokusu üzerine olan toksik etkileri ve
bu toksik etkilere karsı melatonin hormonunun koruyucu etkisi biyokimyasal ve
immunohistokimyasal düzeylerde araştırıldı.
Bu amaçla, 21 adet Wistar-Albino cinsi erkek sıçan üç gruba ayrıldı. Grup
I’deki sıçanlar kontrol olarak kullanıldı. Grup II’deki sıçanlara gün aşırı olarak
formaldehit enjekte edildi. Grup III’deki sıçanlara ise formaldehit enjeksiyonu ile
birlikte melatonin uygulandı. Bir aylık deney süresi sonunda, bütün sıçanlar
dekapitasyon yöntemi ile öldürüldü. Daha sonra, sıçanların testisleri çıkartılarak
çevre dokulardan ayrıldı. Testis doku örneklerinin bir kısmında süperoksit
dismutaz (SOD), glutatyon peroksidaz (GSH-Px) enzim aktiviteleri ile
malondialdehit (MDA) seviyesi spektrofotometrik olarak belirlendi. Testis doku
örneklerinin bir bölümü ise immunohistokimyasal incelemeler için kullanıldı.
Formaldehit uygulanan sıçanlarda SOD ve GSH-Px aktivitelerinin kontrol
grubuna göre anlamlı bir şekilde azaldığı, MDA düzeylerinin ise yine istatistiksel
olarak anlamlı bir şekilde arttığı tespit edildi. Ayrıca formaldehit maruziyeti
sonrası testis dokusunda apoptotik değişikliklerin meydana geldiği
immunohistokimyasal yöntemlerle belirlendi. Formaldehit maruziyeti ile birlikte
melatonin enjekte edilen sıçanlarda ise SOD ve GSH-Px enzim aktivitelerinde bir
artış olurken, MDA değerlerinde istatistiksel olarak anlamlı bir düsüs oldugu
görüldü. Üstelik bu grupta, formaldehit maruziyeti sonucu oluşan apoptotik
değişikliklerin gerilediği tespit edildi.
Sonuç olarak, formaldehit maruziyetine bağlı olarak testis dokusunda
oluşan oksidatif hasarın ve apoptozisin melatonin uygulamasıyla baskılandığı
belirlendi.In our study, toxic effects of formaldehyde on testicular tissue and
protective effects of melatonin hormone against these toxic effects were
investigated at biochemical and immunohistochemical levels.
For this purpose, 21 male Wistar-Albino rats were divided into three
groups. Rats in group I were used as control. Rats in group II were injected every
other day with formaldehyde. Rats in group III were administered melatonin with
injection of formaldehyde. At the end of one month experimental period, all rats
were killed by decapitation. Then the testes of rats were removed and dissected
from the surrounding tissue. The activites of superoxide dismutase (SOD),
glutathione peroxidase (GSH-Px) and the levels of malondialdehyde (MDA) were
determined in the some of testicular tissue specimens by using spectrophotometric
methods. The remaining testicular tissue specimens were used for
immunohistochemical examination.
The activities of SOD and GSH-Px were significantly decreased, and
MDA levels were significantly increased in rats treated with formaldehyde
compared to control. Additionally, apoptotic changes were occurred in testicular
tissue after exposure of formaldehyde. It was seen that increase of SOD and GSHPx
enzyme activities and decrease of MDA levels in rats administered melatonin
with exposure of formaldehyde. Furthermore, apoptotic changes caused by
formaldehyde were regressed in this group.
In conclusion, it was determined that oxidative damage and apoptosis in
testicular tissue caused by exposure of formaldehyde were suppressed by
administration of melatonin