Towards integrated control of varroa: effect of variation in hygienic behaviour among honey bee colonies on mite population increase and deformed wing virus incidence

Hygienic behaviour in the honey bee, Apis mellifera, is the uncapping and removal of dead, diseased or infected brood from sealed cells by worker bees. We determined the effect of hygienic behaviour on varroa population growth and incidence of deformed wing virus (DWV), which can be transmitted by varroa. We treated 42 broodless honey bee colonies with oxalic acid in early January 2013 to reduce varroa populations to low levels, which we quantified by extracting mites from a sample of worker bees. We quantified varroa levels, again when the colonies were broodless, 48 weeks later. During the summer the hygienic behaviour in each colony was quantified four times using the Freeze Killed Brood (FKB) removal assay, and ranged from 27.5 % to 100 %. Varroa population increased greatly over the season, and there was a significant negative correlation between varroa increase and FKB removal. This was entirely due to fully hygienic colonies with >95 % FKB having only 43 % of the varroa build up of the less hygienic colonies.None of the 14 colonies with >80 % FKB removal had overt symptoms of DWV, whilst 36 % of the less hygienic colonies did. Higher levels of FKB removal also correlated significantly with lower numbers of DWV RNA copies in worker bees, but not in varroa mites. On average, fully hygienic colonies had c. 10,000 times less viral RNA than less hygienic colonies

Guleroden i fokus - produktion af frø

Produktion af gulerodsfrø kræver en lang sommer med sol og varme. Regn og rusk giver svampeangreb og dårlige frø, og derfor produceres frø til danske gulerodsmarker i Frankrig eller Italien. Økologiske gulerodsfrø er en mangelvare, men ved dyrkning i tunneler kan der produceres økologiske frø af høj kvalitet i Danmark

LC-MS/MS Quantification Reveals Ample Gut Uptake and Metabolization of Dietary Phytochemicals in Honey Bees (Apis mellifera)

The honey bee pollen/nectar diet is rich in bioactive phytochemicals and recent studies have demonstrated the potential of phytochemicals to influence honey bee disease resistance. To unravel the role of dietary phytochemicals in honey bee health it is essential to understand phytochemical uptake, bioavailability, and metabolism but presently limited knowledge exists. With this study we aim to build a knowledge foundation. For 5 days, we continuously fed honey bees on eight individual phytochemicals and measured the concentrations in whole and dissected bees by HPLC-MS/MS. Ample phytochemical metabolization was observed, and only 6-30% of the consumed quantities were recovered. Clear differences in metabolization rates were evident, with atropine, aucubin, and triptolide displaying significantly slower metabolism. Phytochemical gut uptake was also demonstrated, and oral bioavailability was 4-31%, with the highest percentages observed for amygdalin, triptolide, and aucubin. We conclude that differences in the chemical properties and structure impact phytochemical uptake and metabolism

Parasite infection accelerates age polyethism in young honey bees

Honey bees (Apis mellifera) are important pollinators and their health is threatened worldwide by persistent exposure to a wide range of factors including pesticides, poor nutrition, and pathogens. Nosema ceranae is a ubiquitous microsporidian associated with high colony mortality. We used lab micro-colonies of honey bees and video analyses to track the effects of N. ceranae infection and exposure on a range of individual and social behaviours in young adult bees. We provide detailed data showing that N. ceranae infection significantly accelerated the age polyethism of young bees, causing them to exhibit behaviours typical of older bees. Bees with high N. ceranae spore counts had significantly increased walking rates and decreased attraction to queen mandibular pheromone. Infected bees also exhibited higher rates of trophallaxis (food exchange), potentially reflecting parasite manipulation to increase colony infection. However, reduction in queen contacts could help bees limit the spread of infection. Such accelerated age polyethism may provide a form of behavioural immunity, particularly if it is elicited by a wide variety of pathogens

Honey bee genotypes and the environment

Although knowledge about honey bee geographic and genetic diversity has increased tremendously in recent decades (Meixner et al., 2013), the adaptation of honey bees to their local environment has not been well studied. The current demand for high economic performance of bee colonies with desirable behavioural characteristics contributes to changing the natural diversity via mass importations and an increasing practice of queen trade and colony movement. At the same time, there is also a growing movement in opposition to this trend, aimed at conserving the natural heritage of local populations, with on-going projects in several countries (Strange et al., 2008; Dall’Olio et al., 2008, De la Rúa et al., 2009)

Bierne i Danmark In: Bliv bivenlig

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Population-genetical characteristics of the bee population of Vojvodina

In 1893. Jovan Zivanovic notes that based on the color of the abdomen of bees, two varieties of bees are present in Vojvodina, one dark and the other with two yellow rings on abdomen. Up to the present time there are mixed opinions about the presence of one or more populations of bees in Vojvodina, especially about the presence of the yellow variety in Banat. The aim of this paper is to establish the existence of one or more populations of bees, using genetic analysis of bees. Evaluation of genetic connections, diversity within the population and structure of the bee population in Vojvodina, were calculated on the basis of allele variation of 25 microsatellite loci. A genetic typification of the following microsatellites was performed: A8, A14, A24, A29, A43, A79, A88, A113, Ac11, Ac88, Ac139, Ac306, Ap15, Ap68, Ap85, Ap90, Ap223, Ap224, Ap226, Ap249, Ap273, Ap274, Ap288, At168, At188. 92% or 23 loci proved to be polymorphic in samples of bees from Srem and Backa, and 88% or 22 loci proved to be polymorphic in samples of bees from Banat. Heterozygosity calculated for the whole population is not significantly different from the expected heterozygosity. It was found that the obtained genetic differences between bees of Srem and Backa, and Banat region are not sufficient for these two populations to be considered separate

Reduced SNP panels for genetic identification and introgression analysis in the dark honey bee (Apis mellifera mellifera)

Beekeeping activities, especially queen trading, have shaped the distribution of honey bee (Apis mellifera) subspecies in Europe, and have resulted in extensive introductions of two eastern European C-lineage subspecies (A. m. ligustica and A. m. carnica) into the native range of the M-lineage A. m. mellifera subspecies in Western Europe. As a consequence, replacement and gene flow between native and commercial populations have occurred at varying levels across western European populations. Genetic identification and introgression analysis using molecular markers is an important tool for management and conservation of honey bee subspecies. Previous studies have monitored introgression by using microsatellite, PCR-RFLP markers and most recently, high density assays using single nucleotide polymorphism (SNP) markers. While the latter are almost prohibitively expensive, the information gained to date can be exploited to create a reduced panel containing the most ancestry-informative markers (AIMs) for those purposes with very little loss of information. The objective of this study was to design reduced panels of AIMs to verify the origin of A. m. mellifera individuals and to provide accurate estimates of the level of C-lineage introgression into their genome. The discriminant power of the SNPs using a variety of metrics and approaches including the Weir & Cockerham's FST, an FST-based outlier test, Delta, informativeness (In), and PCA was evaluated. This study shows that reduced AIMs panels assign individuals to the correct origin and calculates the admixture level with a high degree of accuracy. These panels provide an essential tool in Europe for genetic stock identification and estimation of admixture levels which can assist management strategies and monitor honey bee conservation programs