Pulmonary actinomycosis

A case report of pulmonary actinomycosis is presented. The 33-years old man complained of long term pain in the thorax, coughing and subfebrile body temperature. Pleural empyema and pulmonary neoplasm were suspected. The finaly diagnosis was established by open thoracotomy and definitiv ehistological examination. Partiallung resection and post operative penicillin G therapy were performed

Theory and Research From the Communication Field: Discourses That Constitute and Reflect Families

From the disciplinary perspective of communication studies, we review theory and research in family communication, including a brief history of the family communication field; the contributions of a family communication perspective; and 5 theories of family communication: communication accommodation theory, communication privacy management theory, family communication patterns theory, narrative theor(ies), and relational dialectics theory. We then illustrate the concept of discourse dependence in family communication processes and discuss current trends in family communication research. We also suggest emerging directions for family communication scholarship

Regional program of educational suport for talentem pupils and students

Samorząd Województwa Mazowieckiego realizuje ustawowe zadania w kreowaniu polityki oświatowej poprzez Regionalny program wspierania uzdolnionych uczniów i słuchaczy. W ramach tego programu przyznawane są Nagrody Marszałka dla wybitnie uzdolnionej młodzieży z województwa mazowieckiego, we współpracy z organizacjami pozarządowymi finansowany jest zakup pomocy naukowych dla utalentowanych uczniów, będących w trudnej sytuacji materialnej, organizowane są bezpłatne zajęcia, w 6 rejonach województwa, dla młodzieży o uzdolnieniach matematyczno-przyrodniczych i informatycznych. Innymi formami wspierania działań na rzecz podnoszenia wykształcenia jest coroczne przyznanie 3 uczniom uzdolnionym informatycznie pochodzącym z małych miejscowości, bezpłatnych miejsc w Polski-Japońskiej Wyższej Szkole Technik Komputerowych oraz udzielanie pomocy w formie stypendiów współfinansowanych z EFS w ramach Regionalnego programu stypendialnego uczniom uzdolnionym w dziedzinach matematyczno-przyrodniczej i techniczno-zawodowej

Down-regulation of CBP80 gene expression as a strategy to engineer a drought-tolerant potato

Developing new strategies for crop plants to respond to drought is crucial for their innovative breeding. The down-regulation of nuclear cap-binding proteins in Arabidopsis renders plants drought tolerant. The CBP80 gene in the potato cultivar Desiree was silenced using artificial microRNAs. Transgenic plants displayed a higher tolerance to drought, ABA-hypersensitive stomatal closing, an increase in leaf stomata and trichome density, and compact cuticle structures with a lower number of microchannels. These findings were correlated with a higher tolerance to water stress. The level of miR159 was decreased, and the levels of its target mRNAs MYB33 and MYB101 increased in the transgenic plants subjected to drought. Similar trends were observed in an Arabidopsis cbp80 mutant. The evolutionary conservation of CBP80, a gene that plays a role in the response to drought, suggests that it is a candidate for genetic manipulations that aim to obtain improved water-deficit tolerance of crop plants

NPQ assayed in leaf of well-watered potato plants.

<p>Potato WT (dashed line) and transgenic S-7 (solid line) grew in the walk-in growth chamber under controlled conditions and were watered to maintained FC at 65%. Performance of gross non-photochemical quenching (NPQ) were assayed on the first fully developed composite leaf from the top of plant at 4 hours after turning the light with Dual PAM-100. For measurement plants were adapted to dark for 20 minutes and then stimulated with repeated light pulses of actinic light (94 PPFD) for 5 minutes and once again subjected to dark for 6 minutes. Each point represents the mean ±SD (<i>n</i> = 3–4). Experiment was repeated three times and gave comparable results.</p

Accumulation of ROS (hydrogen peroxide and superoxide anion) and lipid peroxidation.

<p>Potato WT (white bars) and transgenic line S-7 (black bars) grew in walk-in growth chamber under controlled conditions. Leaf discs were expunged from the third, fourth and fifth upper fully expanded leaves and immediately vacuum infiltrated with methyl viologen (50 μM). After 1 hour incubation in dark discs were exposed to high light irradiance (850 PPFD) for indicated times (0.5–24 hours). Superoxide anion was determined colorimetrically with nitro blue tetrazolium chloride 9NBT). Hydrogen peroxide was stained in tissue with diaminobenzidine tetrahydrochloride (DAB) and quantified using the ImageJ. Lipid peroxidation was estimated spectrophotometrically with thiobarbituric acid (TBA). Results are means ±SE (n = 5). Homogenic groups are determined by Tukey HSD (Honestly Significant Differences) test. The same letters designate values belong to the same homogenic group (p<0.05). Experiment was repeated twice.</p

Profiling of annexin expression in WT potato leaves during drought.

<p>Potato WT plants grew in the walk-in growth chamber under controlled conditions. After 8–10 weeks irrigation was gradually reduced to decrease the field capacity (FC) to 25% (which took approximately 10 days) and then maintained at this level till 14^th day. Samples were collected from the first fully developed composite leaf from the top at indicated time points (D0 – beginning of drought, D6 – sixth day of drought, and D14 – fourteenth day of drought). RNA was isolated with Trizol and sq-RT-PCR was performed with primer sets specific for certain annexins. The level of expression was normalized against <i>EF1a</i> mRNA. Results are means ±SE (n≤4). Homogenic groups are determined by Tukey HSD (Honestly Significant Differences) test. The same letters designate values belong to the same homogenic group (p<0.05). Experiment was repeated twice.</p