Reconstruction of Intersection Orlovská Street and Betonářská Street in Ostrava

Import 02/11/2016Cílem bakalářské práce je přestavba stávající úrovňové křižovatky ulice Orlovská s ulicí Betonářská v Ostravě na okružní křižovatku. Důvodem přestavby je usnadnění levého odbočení, jak z vedlejší, tak i z hlavní komunikace v důsledku převládající intenzity dopravy v přímém směru. První část se zabývá stávajícím stavem úrovňové křižovatky, provedeným dopravním průzkumem a vyhodnocením dopravní nehodovosti. Druhá část se zabývá návrhem jednotlivých variant okružní křižovatky, jejich kapacitním posouzením a výběrem nejvhodnější varianty. Třetí část se zabývá podrobněji vybranou variantou a posouzením vlečnými křivkami.The goal of bachelor thesis is the reconstruction of the existing intersection Orlovská Street and Betonářská Street in Ostrava to an roundabout. The reason is to simplify left turn from primary road and secondary road due to the prevailing traffic intesity in a straigt line. The fist part deals with the current state of intersection, traffic monitoring and evaluation of traffic accidents. The second part deals with design for the various roundabouts, capacity assessment and selection of the most suitable option. The third part deals with the selected option in more detail and assessment with trawl curves.227 - Katedra dopravního stavitelstvívýborn

Configuration and Verification of HiL Testing Device for Validation of Automotive Image Sensors

Diplomová práce se zabývá možnostmi HiL testování automobilových čelních kamer pro jízdní asistenty. V teoretické části jsou rozebrány jednotlivé snímače jízdních asistentů a možnosti testování automobilových senzorů obrazu. Praktická část je zaměřena na práci s jednotlivými programy od společnosti dSpace v rámci realizace konfigurací pro testovací platformu. V prostředích ConfigurationDesk a ControlDesk jsou vytvořeny aplikace pro nastavení hardwaru SCALEXIO, jenž je zakomponován v HiL zařízení. Pro návrh virtuální 3D scény je využito prostředí ModelDesk a pro vizualizaci MotionDesk. Na závěr je v prostředí AutomationDesk vytvořena vzorová testovací úloha pro validaci funkce čelní kamery MFK3.This master’s thesis describes the possibilities of HiL testing of front cameras for driving assistants. In the theoretical part, the sensors of driving assistants and the possibilities of testing the image of car sensors being individually analysed. The practical part is focused on working with individual programs from dSpace as part of the implementation of configurations for the test platform. Applications for setting up SCALEXIO hardware, which is integrated in the HiL device, are created in the ConfigurationDesk and ControlDesk environments. The ModelDesk environment is used to design a virtual 3D scene and for MotionDesk visualization. Finally, a sample test task for validating the functions of the MFK3 front camera is created in the AutomationDesk environment.430 - Katedra elektronikyvýborn

Magnetic resonance imaging compatible non-invasive fibre-optic sensors based on the Bragg gratings and interferometers in the application of monitoring heart and respiration rate of the human body: A comparative study

The publication presents a comparative study of two fibre-optic sensors in the application of heart rate (HR) and respiratory rate (RR) monitoring of the human body. After consultation with clinical practitioners, two types of non-invasive measuring and analysis systems based on fibre Bragg grating (FBG) and fibre-optic interferometer (FOI) have been designed and assembled. These systems use probes (both patent pending) that have been encapsulated in the bio-compatible polydimethylsiloxane (PMDS). The main advantage of PDMS is that it is electrically non-conductive and, as well as optical fibres, has low permeability. The initial verification measurement of the system designed was performed on four subjects in a harsh magnetic resonance (MR) environment under the supervision of a senior radiology assistant. A follow-up comparative study was conducted, upon a consent of twenty volunteers, in a laboratory environment with a minimum motion load and discussed with a head doctor of the Radiodiagnostic Institute. The goal of the laboratory study was to perform measurements that would simulate as closely as possible the environment of harsh MR or the environment of long-term health care facilities, hospitals and clinics. Conventional HR and RR measurement systems based on ECG measurements and changes in the thoracic circumference were used as references. The data acquired was compared by the objective Bland-Altman (B-A) method and discussed with practitioners. The results obtained confirmed the functionality of the designed probes, both in the case of RR and HR measurements (for both types of B-A, more than 95% of the values lie within the +/- 1.96 SD range), while demonstrating higher accuracy of the interferometric probe (in case of the RR determination, 95.66% for the FOI probe and 95.53% for the FBG probe, in case of the HR determination, 96.22% for the FOI probe and 95.23% for the FBG probe).Web of Science1811art. no. 371

New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats.

Despite the variety of experimental models of spinal cord injury (SCI) currently used, the model of the ventral compression cord injury, which is commonly seen in humans, is very limited. Ventral balloon compression injury reflects the common anatomical mechanism of a human lesion and has the advantage of grading the injury severity by controlling the inflated volume of the balloon. In this study, ventral compression of the SCI was performed by the anterior epidural placement of the balloon of a 2F Fogarty's catheter, via laminectomy, at the level of T10. The balloon was rapidly inflated with 10 or 15 μL of saline and rested in situ for 5 min. The severity of the lesion was assessed by behavioral and immunohistochemical tests. Compression with the volume of 15 μL resulted in severe motor and sensory deficits represented by the complete inability to move across a horizontal ladder, a final Basso, Beattie and Bresnahan (BBB) score of 7.4 and a decreased withdrawal time in the plantar test (11.6 s). Histology and immunohistochemistry revealed a significant loss of white and gray matter with a loss of motoneuron, and an increased size of astrogliosis. An inflation volume of 10 μL resulted in a mild transient deficit. There are no other balloon compression models of ventral spinal cord injury. This study provided and validated a novel, easily replicable model of the ventral compression SCI, introduced by an inflated balloon of Fogarty´s catheter. For a severe incomplete deficit, an inflated volume should be maintained at 15 μL

Allogeneic and autogenous transplantations of MSCs in treatment of the physeal bone bridge in rabbits

<p>Abstract</p> <p>Background</p> <p>The aim of this experimental study on New Zealand's white rabbits was to find differences in the results of treating the distal physeal femoral defect by the transplantation of autologous or allogeneic mesenchymal stem cells (MSCs). After the excision of a created bone bridge in the distal physis of the right femur, modified composite scaffold with MSCs was transplanted into the defect. In animal Group A (n = 11) autogenous MSCs were implanted; in animal Group B (n = 15) allogeneic MSCs were implanted. An iatrogenic physeal defect of the left femur of each animal not treated by MSCs transplantation served as control. The rabbits were euthanized four months after the transplantation. The treatment results were evaluated morphometrically (femoral length and valgus deformity measurement) and histologically (character and quality of the new cartilage).</p> <p>Results</p> <p>Four months after the transplantation, the right femurs of the animals in Group A were on average longer by 0.50 ± 0.04 cm (p = 0.018) than their left femurs, the right femurs of rabbits in Group B were on average longer by 0.43 ± 0.01 cm (p = 0.028) than their left femurs.</p> <p>4 months after the therapeutic transplantation of MSCs valgus deformity of the distal part of the right femur of animals in Group A was significantly lower (by 4.45 ± 1.86°) than that of their left femur (p = 0.028), in Group B as well (by 3.66 ± 0.95° than that of their left femur p = 0.001). However, no significant difference was found between rabbits with transplanted autogenous MSCs (Group A) and rabbits with transplanted allogeneic MSCs (Group B) either in the femur length (p = 0.495), or in its valgus deformity (p = 0.1597). After the MSCs transplantation the presence of a newly formed hyaline cartilage was demonstrated histologically in all the animals (both groups). The ability of transplanted MSCs to survive in the damaged physis was demonstrated in vivo by magnetic resonance, in vitro by Perls reaction and immunofluorescence.</p> <p>Conclusion</p> <p>The transplantation of both autogenous and allogeneic MSCs into a defect of the growth plate appears as an effective method of surgical treatment of physeal cartilage injury. However, the Findings point to the conclusion that there is no clear difference in the final effect of the transplantation procedure used.</p

Analysis of foot and claw diseases/disorders in Czech Holstein cows

Received: 2018-05-07 | Accepted: 2018-05-14 | Available online: 2018-11-26https://doi.org/10.15414/afz.2018.21.04.194-196Foot and claw diseases/disorders from 24 545 lactations of 10 340 Holstein cows were recorded on 7 farms in the Czech Republic from 1999 to 2018. There were defined a three groups of foot and claw disorders/diseases: diseases of skin (SD), which cover digital, interdigital dermatitis and interdigital phlegmon; then disorders of the claw horn (CH) including ulcers, white line disease, horn fissures, together with double sole and  overall claw diseases (OCD) ingluding all the recorded disorders. The OCD ratio observed during 1th and 305th days of lactation was  52.56% of all evaluated lactations. The observed ratio of SD and CH  were 28.61% and 27.15%, respectively. A foot and claw disorders were defined as 0/1 occurrence per lactation, for the purposes of analyses,. Genetic parameters were estimated using by linear animal models for evaluated traits. Models  included the random additive genetic effect of animal (A), the permanent environmental effect of cow (PE), fixed effects of parity, farm, year and season of calving, and age at calving as discreet variables in classes. The estimated heritability were 13.84%, 12.64% and 9.83%, 8.73% and 9.97%, for OCD, CH, SD, ulcers (U) and for dermatitis digitalis and interdigitalis (DD), respectively. Genetic correlation was 17.66% between SD and CH, whereas traits SD and DD equal high genetic similarity (98.4%). Also correlation between CH and U traits was high (92.62%). The presented results indicate to possibility of selection against foot and claw disorders/diseases for Czech Holstein population. The work was supported by the project QJ1510144 and the institutional support MZE-RO0718 of the Ministry of Agriculture of the Czech Republic.Keywords: cattle, foot and claw disorders, genetic parameters, health traits, uddeReferencesBuch, L. H., A. C. Sørensen, J. Lassen, P. Berg, J. A. Eriksson, J. H. Jakobsen,  M. K. Sørensen (2011) Hygiene-related and feed-related hoof diseases show different patterns of genetic correlations to clinical mastitis and female fertility. J. Dairy Sci., 94, 1540-1551.Chapinal, N., A. Koeck, A. Sewalem, D. F. Kelton, S. Mason, G. Cramer,F. Miglior (2013) Genetic parameters for hoof lesions and their relationship with feet and leg traits in Canadian Holstein cows. J. Dairy Sci., 96, 2596-2604.Egger-Danner, C., O.K. Hansen, K. Stock, J.E. Pryce, J. Cole, N. Gengler, B. Heringstad (2013) Challenges and benefits of health data recording in the context of food chain quality, management and breeding. ICAR Technical Series.Groeneveld, E., M. Kovač, and N. Mielenz (2008) VCE User’s Guide and Reference Manual, Version 6.0. Krpálková, L., M. Štípková & M. Krejčová, 2016. Vliv zdraví paznehtů a úrovně reprodukce na výkonnost a zisk stáda dojnic. Náš chov, 76 (9), 58-63.Krupová, Z., Krupa, E., Michaličková, M., Wolfová, M., Kasarda, R. (2016) Economic values for health and feed efficiency traits of dual-purpose cattle in marginal areas. Journal of Dairy Science, ,. 99,  s. 644-656.Madsen, P. , J. Jensen. 2010. DMU – a package for analysing multivariate mixed models. Version 6, release 5.0., Aarhus University, Foulum, Denmark.Pérez-Cabal, M. A. ,N. Charfeddine, N. (2015) Models for genetic evaluations of claw health traits in Spanish dairy cattle.   J. Dairy Sci., 98 (11), 8186-8194.Sogstad, A. M., T. Fjeldaas, O. Østerås,  K. P. Forshell. (2005) Prevalence of claw lesions in Norwegian dairy cattle housed in tie stalls and free stalls. Prev. Vet. Med., 70, 191-209.van der Spek, D., J.A. van Arendonk, A.A. Vallée, H. Bovenhuis (2013) Genetic parameters for claw disorders and the effect of preselecting cows for trimming. J Dairy Sci., 96 (9), 6070-6078.van der Waaij, E. H., M. Holzhauer, E. Ellen, C. Kamphuis, G.de Jong. (2005) Genetic parameters for claw disorders in Dutch dairy cattle and correlations with conformation traits. J. Dairy Sci., 88, 3672-3678

ALANIELUN JA KURKUNPÄÄN ÄÄNTÖVÄYLÄN PINTA-ALOJEN SUHDE ÄÄNIHARJOITUKSISSA JA VOKAALEISSA. TAPAUSTUTKIMUS MAGNEETTIRESONANSSIKUVAUKSEN AVULLA.

Puolisulkuäänteitä ja putkiin ääntämistä käytetään laajasti äänen harjoittamisessa ja terapiassa. Nämä harjoitukset lisäävät ääntöväylänvirtausvastusta. Aiemmat tutkimukset ovat osoittaneet, että [a]-vokaaliinverrattuna putkeen ääntäminen kasvattaa ääntöväylän tilavuutta ja alanielun (hypofarynksin, hf) sisäänmenoaukon pinta-alan suhdetta kurkunpään ääntöväylän (epilaryngaaliputken, e) ulostuloaukon pinta-alaan (Ahf/Ae). Suurella (&gt; 6) Ahf/Ae -suhteella on todettu olevan positiivisia vaikutuksia äänentuottoon ja –laatuun.Tässä tutkimuksessa mitattiin magneettiresonanssikuvauksen avullaAhf/Ae -suhde ja kurkunpään vertikaaliasema kaikissa suomen kielenvokaaleissa ja kolmessa puolisulkuharjoituksessa: kahdella tavalla tuotettu [m:], bilabiaalinen frikatiivi [ß:] ja mehupilliin ääntäminen. Koehenkilö oli naispuolinen puhetekniikan opettaja.Tulosten mukaan nielu oli ääniharjoituksissa useimmiten väljempi ja Ahf/Ae - suhde maksimissaan 63 % suurempi kuin vokaaleissa, poikkeuksena [i:], jossa suhde oli kaikkein suurin (7,3). [ß:] tuotti harjoituksista suurimman Ahf/Ae -suhteen (myös 7,3). Ääniharjoituksista vain kieli alhaalla tuotettu [m] laski kurkunpäätä.Tulokset viittaavat siihen, että eniten ääntöväyläimpedanssia kasvattavatpuolisulkuharjoitukset tuottavat suurimman Ahf/Ae -suhteen, ja että suppeat vokaalit voivat toimia ääniharjoituksina. Kieli ja kurkunpää alennettuna tuotetussa [m]-harjoituksessa Ahf/Ae -suhde oli suurempi kuin kielen etuosa kohotettuna tuotetussa. Tästä syystä edellä mainittu harjoitusvariantti vaikuttaa paremmalta.Avainsanat: puolisulkuharjoitteet, putkeen ääntäminen, ääniterapiaKeywords: resonance tubes, semiocclusion exercises, voice therap

Preparation of progressive antibacterial LDPE surface via active biomolecule deposition approach

The use of polymers in all aspects of daily life is increasing considerably, so there is high demand for polymers with specific properties. Polymers with antibacterial properties are highly needed in the food and medical industries. Low-density polyethylene (LDPE) is widely used in various industries, especially in food packaging, because it has suitable mechanical and safety properties. Nevertheless, the hydrophobicity of its surface makes it vulnerable to microbial attack and culturing. To enhance antimicrobial activity, a progressive surface modification of LDPE using the antimicrobial agent grafting process was applied. LDPE was first exposed to nonthermal radio-frequency (RF) plasma treatment to activate its surface. This led to the creation of reactive species on the LDPE surface, resulting in the ability to graft antibacterial agents, such as ascorbic acid (ASA), commonly known as vitamin C. ASA is a well-known antioxidant that is used as a food preservative, is essential to biological systems, and is found to be reactive against a number of microorganisms and bacteria. The antimicrobial effect of grafted LDPE with ASA was tested against two strong kinds of bacteria, namely, Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), with positive results. Surface analyses were performed thoroughly using contact angle measurements and peel tests to measure the wettability or surface free energy and adhesion properties after each modification step. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used to analyze the surface morphology or topography changes of LDPE caused by plasma treatment and ASA grafting. Surface chemistry was studied by measuring the functional groups and elements introduced to the surface after plasma treatment and ASA grafting, using Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). These results showed wettability, adhesion, and roughness changes in the LDPE surface after plasma treatment, as well as after ASA grafting. This is a positive indicator of the ability of ASA to be grafted onto polymeric materials using plasma pretreatment, resulting in enhanced antibacterial activity. © 2019 by the authors.Qatar National Research Fund (a member of The Qatar Foundation) [JSREP07-022-3-010, NPRP10-0205-170349

Electroproduction, photoproduction, and inverse electroproduction of pions in the first resonance region

Methods are set forth for determining the hadron electromagnetic structure in the sub-$N\bar{N}$-threshold timelike region of the virtual-photon ``mass'' and for investigating the nucleon weak structure in the spacelike region from experimental data on the process $\pi N\to e^+e^- N$ at low energies. These methods are formulated using the unified description of photoproduction, electroproduction, and inverse electroproduction of pions in the first resonance region in the framework of the dispersion-relation model and on the basis of the model-independent properties of inverse electroproduction. Applications of these methods are also shown.Comment: The revised published version; Revtex4, 18 pages, 6 figure

A low-cost system for seismocardiography-based cardiac triggering: A practical solution for cardiovascular magnetic resonance imaging at 3 tesla

This study describes a pilot clinical validation of a new low-cost system for the continuous monitoring of the human body's cardiorespiratory activities within the magnetic resonance examination area. This study primarily focuses on monitoring cardiac activity and the related cardiac triggering. The patented system tested by the authors is based on seismocardiography (SCG). The study was conducted on 18 subjects on a Siemens Prisma 3T MR scanner. Standard anatomical and diffusion sequences were used to test cardiac activity monitoring. A wide range of commonly used diagnostic sequences were used to test imaging of the heart by means of cardiac triggering. System functionality was verified against a commercially available electrocardiography (ECG) system. Monitoring of cardiac activity (detection of the R-R interval in ECG and the AO-AO interval in SCG) was objectively evaluated by determining the overall probability of correct detection (ACC), sensitivity (SE), positive predictive value (PPV), and harmonic mean between SE and PPV, i.e. F1. Imaging quality control using Cardiac Triggering was performed by subjective evaluation of images by the physicians. The study conducted clearly confirmed the functionality of the system in terms of continuous cardiac activity monitoring. In all 18 subjects, a mean PPV > 99% was achieved; F1 > 99 %; SE > 99 %; ACC > 98 %; 1.96 sigma < 3.5 bpm. Also, Cardiac Triggering functionality was confirmed by the physicians on the basis of analyzing cardiac images using the T1/T2 balanced echo sequences and the T1 flash sequence measured natively.Web of Science711862911860