Determination of Total and Individual Anthocyanins in Raspberries Grown in South Serbia

Raspberry fruits of four cultivars (Willamette, Meeker, Polana and Malling Promise) grown in South Serbia were analyzed for total and individual anthocyanins. High-performance liquid chromatography with diode array detection (HPLC-DAD) was used to study individual anthocyanins. The major anthocyanin was cyanidin-3-sophoroside, followed by cyanidin-3-glucosylrutinoside, cyanidin-3-glucoside, pelargonidin-3-sophoroside and cyanidin-3-rutinoside. The monomeric anthocyanin contents of the acidified 80% methanol extracts were determined using the pH-differential method. The highest total and individual anthocyanins contain raspberries cv. Willamette, followed by cvs. Meeker, Polana and Malling Promise

Tobacco and alcohol as factors for male infertility-a public health approach.

BACKGROUND: The study of reproductive characteristics of 430 male subjects of different age, fertility status and educational level who were involved in the program of extracorporeal fertilization at the Clinic of Gynecology and Obstetrics, Clinical Centre Niš, examined their knowledge, attitudes and behavior regarding tobacco and alcohol consumption as lifestyle risk predictors of their partial or full infertility. METHODOLOGY: Consisted of the analyses of spermiograms to establish their fertility status and a survey of their attitudes towards smoking and alcohol use (behavior, knowledge of the general health and reproductive health consequences of such a lifestyle, and their determination to change it). RESULTS: The proportion with higher tobacco consumption and more severe forms of infertility increased significantly with ageing (P < 0.001); the highest daily consumption of alcohol and the incidence of intoxication was seen among azoospermic patients; the level of awareness of the harmful effects of tobacco was highest among normozoospermic subjects and the highest level of determination to quit smoking was statistically significantly present among azoospermic subjects. CONCLUSION: Appropriate use of health promotion activities in relation to alcohol and tobacco use is through specially designed programs

Leadership and motivation among employees in health-care facilities in Serbia

Reserarch question: This paper investigates whether motivational factors have influence on the work of employees in health care organizations of Serbia and what factors affect employees. Motivation: Motivation and job satisfaction among different groups of workers in health care facilities in Serbia are an important issue. This study’s purpose is to assist health managers in their efforts to fulfill individual and organizational targets by highlighting the most preferred motivational factors among the employees. With good leadership and with the building of good motivational system the organization can increase its value and competitiveness. It has been shown that employees are more motivated and work under less stress if there is a support from their leader or manager (Jensen, 2010). Idea: The  core  idea  of  this  paper  is to  evaluate  the  relationship  between motivational factors and work of employees in health care institutions in Serbia. The central hypothesis of this study is what the managers within health care institutions have to do to improve motivation as well as their abilities for the fulfillment of both individual’s and organization’s needs. Data: The cross-sectional study included 217 physicians, nurses, technicians, health associates and non-medical staff in 21 facilities of primary, secondary and tertiary levels in Pozarevac, Belgrade, Nis, Pirot, Novi Sad, Zrenjanin, Krusevac, Varvarin and Novi Pazar. Tools: The research instrument was a questionnaire with questions regarding socio-demographic characteristics, participants’ characteristics and motivational factors. The data were analyzed using descriptive and analytical statistics. Findings: The most important motivational factors are: salary, good interpersonal relationships and team work, contribution to population’s health and patient’s satisfaction. Around 10% of the participants thought that work could be done well even without motivational factors’ presence. Employees from the areas outside Belgrade rated job security higher in comparison with employees from Belgrade. A study conducted at the University of llorin Teaching Hospital in 2011 showed that salary was the most important motivational factor, followed by job security (Ojokuku& Salami, 2011). Contribution: Salary is an important motivational factor for employees in health care facilities in Serbia. Managers should work on the improvement of motivational factors through acknowledgement of needs of individual employees as well as through involving employees into decision making

Vrste roda Monilinia na jabučastim i koštičavim vrstama voćaka

Different species of the genus Monilinia are common plant pathogens that endanger pome and stone fruit production worldwide. In Serbia, two species of this genus are widely distributed – M. laxa and M. fructigena, while M. fructicola, which is officially on the A2 EPPO List of quarantine pest organisms in Europe and on the 1A part I List of quarantine pest organisms in Serbia, has so far been detected only on stored apple and nectarine fruits. The most important control measures against these pathogens include chemical control in combination with adequate cultural practices, particularly under favourable conditions for disease development. Concerning that species of this genus can cause significant economic losses, knowledge of the pathogen biology, disease epidemiology and pathogen-host interactions is a necessary prerequisite for stable and profitable production of pome and stone fruits.Različite vrste roda Monilinia dobro su poznati biljni patogeni koji ugrožavaju proizvodnju jabučastog i koštičavog voća širom sveta. U našoj zemlji široko su rasprostranjene dve vrste ovog roda – M. laxa i M. fructigena, dok je M. fructicola koja se zvanično nalazi na A2 EPPO listi karantinskih štetnih organizama u Evropi i na A1 listi karantinskih štetnih organizama u našoj zemlji, do sada detektovana samo na uskladištenim plodovima jabuke i plodovima nektarine. Najznačajnija mera borbe protiv ovih patogena je hemijsko suzbijanje uz primenu adekvatnih agrotehničkih mera, naročito u godinama povoljnim za razvoj oboljenja. S obzirom da vrste ovog roda mogu prouzrokovati velike ekonomske gubitke, stepen poznavanja biologije patogena, epidemiologije bolesti i odnosa parazit-domaćin predstavljaju neophodan preduslov stabilne i profitabilne proizvodnje jabučastog i koštičavog voća

Simultaneous Administration of Hyperbaric Oxygen Therapy and Antioxidant Supplementation with <i>Filipendula ulmaria</i> Extract in the Treatment of Thermal Skin Injuries Alters Nociceptive Signalling and Wound Healing

Background and Objectives: Thermal skin injuries are a prevalent cause of skin damage, potentially leading to severe morbidity and significant mortality. In this study, we intended to estimate the effects of HBO (hyperbaric oxygen treatment) and antioxidant supplementation with Filipendula ulmaria extract, individually and simultaneously, in the treatment of thermal skin injuries. Materials and Methods: As a thermal skin injury experimental model, we used two-month-old male Wistar albino rats. Thermal injuries were made with a solid aluminium bar at a constant temperature of 75 °C for 15 s. Hyperbaric oxygen treatment was performed in a specially constructed hyperbaric chamber for rats (HYB-C 300) for seven consecutive days (100% O2 at 2.5 ATA for 60 min). Antioxidant supplementation was performed with oral administration of Filipendula ulmaria extract dissolved in tap water to reach a final concentration of 100 mg/kg b.w. for seven consecutive days. Results: Simultaneous administration of hyperbaric oxygen therapy and antioxidant supplementation with Filipendula ulmaria extract significantly ameliorated the macroscopic and histopathological characteristics of the wound area and healing. Also, this therapeutic approach decreased the local expression of genes for proinflammatory mediators and increased the expression of the μ-opioid receptor and the MT1 and MT2 receptors in the wound area and spinal cord, with a consequent increase in reaction times in behavioural testing. Conclusions: In conclusion, the presented results of our study allow evidence for the advantages of the simultaneous employment of HBO and antioxidant supplementation in the treatment of thermal skin injuries, with special reference to the attenuation of painful sensations accompanied by this type of trauma

Design and construction of wall-less nano-electrophoretic and nano in micro array high throughput devices for single cell 'omics' single molecule detection analyses

cited By 0International audienceSingle cell 'omics' requires a technological platform with reliable and high throughput single cell analyses with single molecular detection and quantification. Presently available options are to either to detect many different macromolecules and metabolites extracted from many cells, thus obtaining partial 'omics' of an average cell or to study only few single cells and be limited to semi-quantitative analyses and detection of a few abundant molecules. Here we present a new design and prototype proof of concept construction of high throughput nano-electrophoretic separation (NEA) device and nano in micro array (NiMA) affinity probe device for a complete single cell 'omics' single bio-molecule polymers detection and quantification analyses. Prototype devices were constructed using gallium ion Focus Ion Beam (FIB), Gas Injection System (GIS) and Scanning Electron Microscope (SEM) crossbeam instruments. The NEA device accommodates 100 different cell samplings per 1 cm2 chip with arrays of open nano-electrophoretic guides. The NiMA bio-sensor device on 1 cm2 can accommodate 2500 cells in a micro-well array which consists of 250,000 probe markers in nano-well array located in each micro-well. Using Secondary Ion Mass Spectrometry (SIMS) we have demonstrated the direct detection of a single protein molecule and proved the feasibility of single bio-molecular detection and quantification concept for NEA and NIMA. Our concept validates high throughput and complete and quantitative single cell 'omics' with single molecular detection analyses without labeling. Thus, it is superior to commonly used microfluidics, capillary electrophoresis and micro-arrays using mass spectrometry and fluorescent labeling for molecular detection

LM Fitted Parameters for Impedance Models C2, GC2, C1GC1.

<p>LM Fitted Parameters for Impedance Models C2, GC2, C1GC1.</p

Schema of circuit for modified Cole element [20] according to Magin [7], [8].

<p>Schema of circuit for modified Cole element <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059483#pone.0059483-Cole1" target="_blank">[20]</a> according to Magin <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059483#pone.0059483-Magin1" target="_blank">[7]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059483#pone.0059483-Magin2" target="_blank">[8]</a>.</p

Schema of Circuit for all Permutation of Serially Linked Reduced Generalized Cole Elements and Reduced Cole elements.

<p>Schema of Circuit for all Permutation of Serially Linked Reduced Generalized Cole Elements and Reduced Cole elements.</p