Volunteer based organizations: bridging the gap between political efficacy and civic engagement

With the declining participation in civic engagement behaviors such as voting, people are turning to other means to demonstrate civic participation. This study examined the role of volunteering as a means to establish civic engagement. Because of this civic participation behavioral shift, the relationship between civic engagement and the communication tactics used by volunteer based organizations was investigated. This study investigated the relationship between civic engagement and political efficacy. Additionally, this study examined the interplay between organizational characteristics (trust, control mutuality, exchange relationship, and commitment) and civic engagement. Lastly, perceptions of new and traditional methods of communications to encourage civic engagement and build trust of volunteer based organizations were explored. To address these relationships, this study used on online survey with 245 adults and the data were analyzed using a linear regression analysis and SPSS Textual Analysis for Surveys. This study used convenience and snowball sampling. This survey used various civic engagement scales and James Grunig’s PR Measurement Scale for organizational factors. Findings show higher levels of participation of political interest and trust lead to higher levels of political efficacy. Additionally, stronger control mutuality beliefs of an organization lead to higher levels of civic engagement. Participants reported email and direct mail to be the methods of communication they are most familiar with from their volunteer based organizations that encourage engagement. Furthermore, ease and access to information are crucial for volunteers regarding engagement and trust

Characterization of the effect of the menstrual cycle, estradiol and Fads2 transcript variants on fatty acid composition and enzyme expression

Estrogen is believed to regulate fatty acid composition based on observations of differences between men and women, and changes associated with altered estrogen levels in women during pregnancy, menopause and hormone treatment. Estrogen is consistently associated with increased levels of 18:0, 16:1n-7 and elongase 6, an enzyme in monounsaturated fatty acids (MUFA) biosynthesis. In some, but not all studies, estrogen has also been associated with increased 22:6n-3 and FADS2 expression. FADS2 is responsible for delta-6 desaturase activity, the rate-limiting step in polyunsaturated fatty acid (PUFA) biosynthesis and is necessary for the synthesis of 22:6n-3, which is critical for cardiovascular health and neural development. In this thesis, the effects of subtle changes in estrogen levels during the menstrual cycle in humans, and the estrous cycle in rats, on fatty acid metabolism was examined. In addition, the response of FADS2 to estrogen was examined using MCF7 cells. The presence of multiple FADS2 transcript variants in MCF7 cells was determined, and the role of two variants in PUFA biosynthesis was examined by transfecting them into HEK 293 cells. During the menstrual cycle in women and estrous cycle in rats, increased blood levels of 16:1n-7 and 22:6n-3, and decreased levels of 22:5n-3 were observed when blood estrogen levels were high. The increased 22:6n-3 and decreased 22:5n-3 suggests an effect of estrogen on FADS2 expression and/or activity. In the rat hepatic expression of FADS2 mRNA varied over the cycle, but protein levels did not. However, in estrogen sensitive MCF7 cells, FADS2 mRNA was induced by estrogen. Changes in 22:6n-3 and 22:5n-3 were not observed in the MCF7 cells. Further examination identified multiple transcript variants of FADS2 in MCF7 cells, and demonstrated that the expression of an alternatively spliced transcript variant (FADS2_v2) was considerably higher. Notably, this variant lacked the N-terminal cytochrome-binding domain that is critical for activity, suggesting that it may not be functional. In transfected HEK 293 cells, indirect evidence of delta-6 desaturase activity was observed for the full length wild type variant (FADS2_v0/1) but not for FADS2_v2. When the variants were co-transfected, FADS2_v2 interacted with and inhibited FADS2_v0/1. This work helps further understanding of the relationship between estrogen, lipid biosynthetic enzymes and fatty acid status that is important for determination of dietary requirements of 22:6n-3 for women throughout the lifespan

Examination of the Effects of Sex and Sex Hormones on Monounsaturated Fatty Acid Biosynthesis

Monounsaturated fatty acids (MUFA) have been viewed as either beneficial or neutral with respect to health; however, recent evidence suggests that MUFA may be associated with obesity and cardiovascular disease. Sex differences in MUFA composition have been reported in both rats and humans that may be mediated by sex hormones. Therefore, differences in fatty acid and enzyme expression were examined in male and female rats. HepG2 cells treated with 17β-estradiol, progesterone and testosterone (0, 10, 30 and 100 nM), and ovariectomized female rats with hormone implants (sham, no treatment, estradiol, progesterone, and estradiol plus progesterone) examined the role of sex hormones in MUFA metabolism. MUFA concentrations were determined by gas chromatography. The mRNA and protein expression of stearoyl-CoA desaturase and elongase 6, key enzymes involved in MUFA biosynthesis, were measured by real-time PCR and immunoblotting, respectively. Elongase 6 protein expression was higher in females as compared with males, increased with estradiol and progesterone treatment of HepG2 cells, and was higher in ovariectomized rats treated with estradiol. Elongase 6 expression was also decreased in HepG2 cells treated with testosterone. In contrast, the expression of stearoyl-CoA desaturase did not appear to be associated with sex or sex hormones in HepG2 cells, although ovariectomized rats treated with estradiol plus progesterone had increased stearoyl-CoA desaturase protein levels compared with sham controls. Sex differences and differences after hormonal treatments were observed in the fatty acid concentrations of MUFA and immediate MUFA substrates including 16:0, 16:1n-7, 18:0, 18:1n-7 and 18:1n-9. These differences in MUFA are consistent with the changes in elongase 6 expression. The effect of sex and hormone status on elongase 6 has not been previously examined, but these results suggest elongase 6 is an important factor in determining MUFA composition. These sex and hormonal differences in MUFA composition may contribute to sex differences in obesity and cardiovascular disease. Further work examining other factors involved in MUFA composition including oxidation, synthesis of complex lipids and the effect of diet is required

Senior Recital: Chris Marks

Senior Recital: Chris Markshttps://digitalcommons.kennesaw.edu/musicprograms/2444/thumbnail.jp

Prenatal exposure to mixtures of persistent endocrine disrupting chemicals and early menarche in a population-based cohort of British girls

Exposure to endocrine disrupting chemicals (EDCs) is ubiquitous. EDC exposure, especially during critical periods of development like the prenatal window, may interfere with the body’s endocrine system, which can affect growth and developmental outcomes such as puberty. Most studies have examined one EDC at a time in relation to disease; however, humans are exposed to many EDCs. By studying mixtures, the human experience can be more closely replicated. We investigated the association of prenatal exposure to persistent EDCs (poly- and perfluoroalkyl substances (PFAS), polychlorinated biphenyls (PCBs), and organochlorine pesticides (OCPs)) as mixtures with early menarche among female offspring in a nested case-control study within the Avon Longitudinal Study of Parents and Children (ALSPAC) recruited in the United Kingdom in 1991–1992. Concentrations of 52 EDCs were quantified in maternal serum samples collected during pregnancy. Daughter’s age at menarche was ascertained through mailed questionnaires sent annually. We used repeated holdout weighted quantile sum (WQS) regression and Bayesian kernel machine regression (BKMR) to examine the association between prenatal exposure to multiple EDCs and early menarche (<11.5 (n = 218) vs. ≥11.5 years (n = 230)) for each chemical class separately (PFAS, PCBs, and OCPs) and for all three classes combined. Models adjusted for maternal age at menarche, maternal education, parity, pre-pregnancy body mass index, maternal age, prenatal smoking, and gestational week at sample collection. Mixture models showed null associations between prenatal exposure to EDC mixtures and early menarche. Using WQS regression, the odds ratio for early menarche for a one-decile increase in chemical concentrations for all three classes combined was 0.89 (95% CI: 0.76, 1.05); using BKMR, the odds ratio when all exposures were at the 60th percentile compared to the median was 0.98 (95% CI: 0.91, 1.05). Results suggest the overall effect of prenatal exposure to persistent EDC mixtures is not associated with early menarche

Local recurrence after surgery for non–small cell lung cancer: A recursive partitioning analysis of multi-institutional data

OBJECTIVE: To define subgroups at high risk of local recurrence (LR) after surgery for non-small cell lung cancer using a recursive partitioning analysis (RPA). METHODS: This Institutional Review Board-approved study included patients who underwent upfront surgery for I-IIIA non-small cell lung cancer at Duke Cancer Institute (primary set) or at other participating institutions (validation set). The 2 data sets were analyzed separately and identically. Disease recurrence at the surgical margin, ipsilateral hilum, and/or mediastinum was considered an LR. Recursive partitioning was used to build regression trees for the prediction of local recurrence-free survival (LRFS) from standard clinical and pathological factors. LRFS distributions were estimated with the Kaplan-Meier method. RESULTS: The 1411 patients in the primary set had a 5-year LRFS rate of 77% (95% confidence interval [CI], 0.74-0.81), and the 889 patients in the validation set had a 5-year LRFS rate of 76% (95% CI, 0.72-0.80). The RPA of the primary data set identified 3 terminal nodes based on stage and histology. These nodes and their 5-year LRFS rates were as follows: (1) stage I/adenocarcinoma, 87% (95% CI, 0.83-0.90); (2) stage I/squamous or large cell, 72% (95% CI, 0.65-0.79); and (3) stage II-IIIA, 62% (95% CI, 0.55-0.69). The validation RPA identified 3 terminal nodes based on lymphovascular invasion (LVI) and stage: (1) no LVI/stage IA, 82% (95% CI, 0.76-0.88); (2) no LVI/stage IB-IIIA, 73% (95% CI, 0.69-0.80); and (3) LVI, 58% (95% CI, 0.47-0.69). CONCLUSIONS: The risk of LR was similar in the primary and validation patient data sets. There was discordance between the 2 data sets regarding the clinical factors that best segregate patients into risk groups

Dietary Oxidative Balance Scores and Biomarkers of Inflammation among Individuals with and without Chronic Kidney Disease

Background: Oxidative stress and inflammation are proposed mechanisms of nonspecific kidney injury and progressive kidney failure. Higher dietary oxidative balance scores (OBS) are associated with lower prevalence of chronic kidney disease (CKD). Methods: We investigated the association between OBS and biomarkers of inflammation using data from the Reasons for Geographic and Racial Differences in Stroke (REGARDS) study. Nutrient estimates from the Block Food Frequency Questionnaires were used to define tertiles of 11 pro- and antioxidant factors. Points for each OBS component were summed, with a higher score indicating predominance of antioxidant exposures. Multivariable linear regression models were used to estimate the association between OBS and biomarkers of inflammation (interleukin-6 [IL-6], interleukin-8 [IL-8], interleukin-10 [IL-10], fibrinogen, C-reactive protein [CRP], white blood cell count, and cystatin C). An interaction term was included to determine if associations between OBS and inflammatory markers differed between individuals with and without CKD. Results: Of 682 participants, 22.4% had CKD. In adjusted models, OBS was associated with CRP and IL-6. For every 5-unit increase in OBS, the CRP concentration was –15.3% lower (95% CI: –25.6, –3.6). The association of OBS with IL-6 differed by CKD status; for every 5-unit increase in OBS, IL-6 was –10.7% lower (95% CI: –16.3, –4.7) among those without CKD, but there was no association among those with CKD (p = 0.03). Conclusion: This study suggests that a higher OBS is associated with more favorable levels of IL-6 and CRP, and that the association of OBS and IL-6 may be modified by CKD status

Can an hour or two of sun protection education keep the sunburn away? Evaluation of the Environmental Protection Agency's Sunwise School Program

BACKGROUND: Melanoma incidence is rising at a rate faster than any other preventable cancer in the United States. Childhood exposure to ultraviolet (UV) light increases risk for skin cancer as an adult, thus starting positive sun protection habits early may be key to reducing the incidence of this disease. METHODS: The Environmental Protection Agency's SunWise School Program, a national environmental and health education program for sun safety of children in primary and secondary schools (grades K-8), was evaluated with surveys administered to participating students and faculty. RESULTS: Pretests (n = 5,625) and posttests (n = 5,028) were completed by students in 102 schools in 42 states. Significant improvement was noted for the three knowledge variables. Intentions to play in the shade increased from 68% to 75%(p < 0.001) with more modest changes in intentions to use sunscreen. Attitudes regarding healthiness of a tan also decreased significantly. CONCLUSIONS: Brief, standardized sun protection education can be efficiently interwoven into existing school curricula, and result in improvements in knowledge and positive intentions for sun protection