Map of the mobilization helper plasmid pTA-Mob with relevant regions depicted.

<p>Gm^r, gentamycin resistance gene; <i>rep</i>, pBBR1 replication protein gene; <i>ori</i>; pBBR1 replication origin; (<i>trfA</i>), replication initiation protein gene from the RK2 replicon, this replicon is not active due to lack of RK2 replication origin <i>oriV</i>; Tra1 and Tra2, regions containing the <i>tra</i> genes necessary for conjugative transfer of <i>oriT</i> containing plasmids; <i>parABCDE</i>, stabilization region encoding the gene products ParA, B, C, D and E; Ctl, central control operon of RK2 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090372#pone.0090372-Pansegrau1" target="_blank">[6]</a>.</p

Agarose gel electrophoresis analysis of HindIII-digested fosmid clones before and after conjugation from <i>E. coli</i> S17-1 to <i>P. fluorescens::</i>Tn<i>RS48</i> or <i>X. campestris::</i>Tn<i>RS48</i>.

<p>Lane 1, plasmid 62 before transfer, and lanes 2 and 3 after transformation to <i>E. coli</i> from <i>P. fluorescens</i> and <i>X. campestris</i>, respectively. Lane 4, plasmid 83 before conjugal transfer, and after transformation to <i>E. coli</i> from <i>P. fluorescens</i> (lanes 5-7) and <i>X. campestris</i> (lanes 8 and 9). Lane 10, plasmid 37 before transfer, and after transformation to <i>E. coli</i> from <i>P. fluorescens</i>. (lanes 11 and 12) and <i>X. campestris</i> (lane 13). S: Molecular weight standard (Fermentas).</p

Agarose gel electrophoresis analysis of HindIII-digested fosmid clones that have been conjugatively transferred from <i>E. coli</i> DH10B/pTA-Mob to <i>P. fluorescens::</i>Tn<i>RS48</i>.

<p>Lane 1: plasmid 37 before transfer and lanes 2-4 after conjugation. Lane 5: Plasmid 83 before transfer and lanes 6–8 after conjugation. S: Molecular weight standard (NEB).</p