Insect Pheromone Receptors – Key Elements in Sensing Intraspecific Chemical Signals

Pheromones are chemicals that serve intraspecific communication. In animals, the ability to detect and discriminate pheromones in a complex chemical environment substantially contributes to the survival of the species. Insects widely use pheromones to attract mating partners, to alarm conspecifics or to mark paths to rich food sources. The various functional roles of pheromones for insects are reflected by the chemical diversity of pheromonal compounds. The precise detection of the relevant intraspecific signals is accomplished by specialized chemosensory neurons housed in hair-like sensilla located on the surface of body appendages. Current data indicate that the extraordinary sensitivity and selectivity of the pheromone-responsive neurons (PRNs) is largely based on specific pheromone receptors (PRs) residing in their ciliary membrane. Besides these key elements, proper ligand-induced responses of PR-expressing neurons appear to generally require a putative co-receptor, the so-called “sensory neuron membrane protein 1” (SNMP1). Regarding the PR-mediated chemo-electrical signal transduction processes in insect PRNs, ionotropic as well as metabotropic mechanisms may be involved. In this review, we summarize and discuss current knowledge on the peripheral detection of pheromones in the olfactory system of insects with a focus on PRs and their specific role in the recognition and transduction of volatile intraspecific chemical signals

The Sensilla-Specific Expression and Subcellular Localization of SNMP1 and SNMP2 Reveal Novel Insights into Their Roles in the Antenna of the Desert Locust Schistocerca gregaria

The desert locust, Schistocerca gregaria, can form gigantic swarms of millions of individuals that devastate the vegetation of invaded landscapes. Locust food search, reproduction, and aggregation behaviors are triggered and controlled by complex olfactory signals. Insects detect odorants through different types of olfactory sensilla on the antenna that house olfactory sensory neurons and associated support cells, both of which express the proteins required for olfactory signaling. Among these proteins, two members of the CD36 lipid transporter/receptor family, named sensory neuron membrane proteins 1 and 2 (SNMP1 and SNMP2), are indicated to be of vital importance. Towards a better understanding of the role of the two SNMPs in the olfactory system of S. gregaria, we have analysed their antennal topography and subcellular localization using specific antibodies. The results indicate sensilla type- and cell type-specific distribution patterns of the SNMP proteins. SNMP1 was located in the receptive dendrites of subpopulations of olfactory sensory neurons as well as in the microvilli of associated support cells, suggesting a dual function of this protein, both in olfactory signal detection and in sensillum lymph maintenance, respectively. In contrast, SNMP2 was found solely in support cells and their microvilli membranes, suggesting a role limited to sensillum lymph recovery processes.Insect olfactory sensilla house olfactory sensory neurons (OSNs) and supports cells (SCs). The olfactory sensory processes require, besides the odorant receptors (ORs), insect-specific members of the CD36 family, named sensory neuron membrane proteins (SNMPs). While SNMP1 is considered to act as a coreceptor in the OR-mediated detection of pheromones, SNMP2 was found to be expressed in SCs; however, its function is unknown. For the desert locust, Schistocerca gregaria, we previously visualized mRNA for SNMP1 in OSNs and SNMP2 mRNA in cells associated with OSN clusters. Towards an understanding of their functional implication, it is imperative to explore the cellular and the subcellular localization the SNMP proteins. Therefore, we have generated polyclonal antibodies against SNMP1 and SNMP2 and used fluorescence immunohistochemistry (FIHC) to visualize the SNMP proteins. We found SNMP1 in the somata and respective dendrites of all OSNs in trichoid sensilla and in subsets of OSNs in basiconic sensilla. Notably, SNMP1 was also detected in SCs of these sensilla types. In contrast, SNMP2 protein was only visualized in SCs of basiconic and coeloconic sensilla, but not of trichoid sensilla. Exploring the subcellular localization by electron microscopy using anti-SNMP1-ab and anti-SNMP2-ab revealed an immunogold labelling of SC microvilli bordering the sensillum lymph. Together our findings suggest a dual role of SNMP1 in the antenna of S. gregaria, in some OSN subpopulations in odor detection as well as in functions of some SCs, whereas the role of SNMP2 is limited to the functions of support cells.Peer Reviewe

Pathogens in ticks collected from dogs in Berlin/Brandenburg, Germany

BackgroundTick-borne diseases are a major health risk for humans and dogs. In addition to collection and analysis of questing ticks, analysis of host- associated ticks for the presence of pathogens is a valuable method to gain insight into transmission patterns of tick-borne diseases.MethodsTicks were collected from dogs living in the Berlin/Brandenburg area. The three tick species Ixodes ricinus, Ixodes hexagonus and Dermacentor reticulatus were examined for the presence of Babesia spp., Borrelia spp., Rickettsia spp. and Anaplasmataceae. Conventional PCR followed by sequencing was used for pathogen detection and characterization.Results Babesia spp. were found in 2.5% and 3% of I. ricinus and I. hexagonus, respectively. Sequencing revealed the presence of Babesia microti, Babesia capreoli and Babesia venatorum. D. reticulatus were free of Babesia canis. Rickettsia spp. were detected in 61% of I. ricinus, 44% of I. hexagonus and 39% of D. reticulatus. Specifically detected were Rickettsia raoulti in D. reticulatus and I. hexagonus, Rickettsia helvetica in I. ricinus and I. hexagonus and Rickettsia monacensis in I. hexagonus. Anaplasma phagocytophilum and Candidatus Neoehrlichia mikurensis have been reported previously in I. ricinus (6.5% and 4.3%, respectively) and I. hexagonus (3.9% and 5.9%). Borrelia spp. were found in 11.6% of I. ricinus and 11.2% of I. hexagonus. Subsequent genospecies analysis revealed Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto and Borrelia miyamotoi. Simultanous presence of more than one pathogen was found in 20% of I. ricinus and in 59% of I. hexagonus whereas the total frequency of any pathogen was 65% in I. ricinus, 59% in I. hexagonus and 64% in D. reticulatus. Ticks in which A. phagocytophilum was detected had a significantly increased risk of also containing Rickettsia. Ticks harbouring a pathogen had significantly higher scutal indices than ticks without presence of any pathogen.ConclusionsFrequencies of potential human or canine pathogens in ticks were considerable and DNA of all four groups of pathogens was detected. Differences in scutal indices might suggest that pathogens are frequently taken up by ticks when feeding on dogs in Berlin/Brandenburg

The Molecular ISM in the Super Star Clusters of the Starburst NGC 253

We present submillimeter spectra of the (proto-)super star cluster (SSC) candidates in the starbursting center of the nearby galaxy NGC 253 identified by Leroy et al. (2018). The 2.5pc resolution of our ALMA cycle 3 observations approach the size of the SSCs and allows the study of physical and chemical properties of the molecular gas in these sources. In the 14 SSC sources and in the frequency ranges 342.0-345.8 GHz and 353.9-357.7 GHz we detect 55 lines belonging to 19 different chemical species. The SSCs differ significantly in chemical complexity, with the richest clusters showing 19 species and the least complex showing 4 species. We detect HCN isotopologues and isomers (H$^{13}$CN, HC$^{15}$N, H$^{15}$NC), abundant HC$_3$N, SO and S$^{18}$O, SO$_2$, and H$_2$CS. The gas ratios CO/HCN, CO/HCO$^+$ are low, ~1-10, implying high dense gas fractions in the SSCs. Line ratio analyses suggests chemistry consistent with photon-dominated regions and mechanical heating. None of the SSCs near the galaxy center show line ratios that imply an X-ray dominated region, suggesting that heating by any (still unknown) AGN does not play a major role. The gas temperatures are high in most sources, with an average rotational temperature of ~130 K in SO$_2$. The widespread existence of vibrationally excited HCN and HC$_3$N transitions implies strong IR radiation fields, potentially trapped by a greenhouse effect due to high continuum opacities.Comment: 20 pages, 4 figures, 6 tables; accepted for publication in the Astrophysical Journa

The turbulent gas structure in the centers of NGC253 and the Milky Way

We compare molecular gas properties in the starbursting center of NGC253 and the Milky Way Galactic Center (GC) on scales of ~1-100 pc using dendograms and resolution-, area- and noise-matched datasets in CO (1-0) and CO (3-2). We find that the size-line width relations in NGC253 and the GC have similar slope, but NGC253 has larger line widths by factors of ~2-3. The $\sigma^2/R$ dependency on column density shows that, in the GC, on scales of 10-100 pc the kinematics of gas over $N>3\times10^{21}$ cm$^{-2}$ are compatible with gravitationally bound structures. In NGC253 this is only the case for column densities $N>3\times10^{22}$ cm$^{-2}$. The increased line widths in NGC253 originate in the lower column density gas. This high-velocity dispersion, not gravitationally self-bound gas is likely in transient structures created by the combination of high average densities and feedback in the starburst. The high densities turns the gas molecular throughout the volume of the starburst, and the injection of energy and momentum by feedback significantly increases the velocity dispersion at a given spatial scale over what is observed in the GC.Comment: 13 pages, 3 figures, 4 tables; accepted for publication in the Astrophysical Journa

The Molecular Outflow in NGC 253 at a Resolution of Two Parsecs

We present 0.″15 (̃2.5 pc) resolution ALMA CO(3-2) observations of the starbursting center in NGC 253. Together with archival ALMA CO(1-0) and CO(2-1) data, we decompose the emission into disk and nondisk components. We find ̃7%-16% of the CO luminosity to be associated with the nondisk component (1.2-4.2 × 107 K km s-1 pc2). The total molecular gas mass in the center of NGC 253 is ̃3.6 × 108 M ☉ with ̃0.5 × 108 M ☉ (̃15%) in the nondisk component. These measurements are consistent across independent mass estimates through three CO transitions. The high-resolution CO(3-2) observations allow us to identify the molecular outflow within the nondisk gas. Using a starburst conversion factor, we estimate the deprojected molecular mass outflow rate, kinetic energy, and momentum in the starburst of NGC 253. The deprojected molecular mass outflow rate is in the range of ̃14-39 M ☉ yr-1 with an uncertainty of 0.4 dex. The large spread arises due to different interpretations of the kinematics of the observed gas while the errors are due to unknown geometry. The majority of this outflow rate is contributed by distinct outflows perpendicular to the disk, with a significant contribution by diffuse molecular gas. This results in a mass-loading factor η ={\dot{M}}out}/{\dot{M}}SFR} in the range η ̃ 8-20 for gas ejected out to ̃300 pc. We find the kinetic energy of the outflow to be ̃2.5-4.5 × 1054 erg and a typical error of ̃0.8 dex, which is ̃0.1% of the total or ̃8% of the kinetic energy supplied by the starburst. The outflow momentum is 4.8-8.7 × 108 M ☉ km s-1 (̃0.5 dex error) or ̃2.5%-4% of the kinetic momentum released into the ISM by the feedback. The unknown outflow geometry and launching sites are the primary sources of uncertainty in this study.</p