22,081 research outputs found
A rapid staining-assisted wood sampling method for PCR-based detection of pine wood nematode Bursaphelenchus xylophilus in Pinus massoniana wood tissue
For reasons of unequal distribution of more than one nematode species in wood, and limited
availability of wood samples required for the PCR-based method for detecting pinewood nematodes in
wood tissue of Pinus massoniana, a rapid staining-assisted wood sampling method aiding PCR-based
detection of the pine wood nematode Bursaphelenchus xylophilus (Bx) in small wood samples of P.
massoniana was developed in this study. This comprised a series of new techniques: sampling, mass
estimations of nematodes using staining techniques, and lowest limit Bx nematode mass determination
for PCR detection. The procedure was undertaken on three adjoining 5-mg wood cross-sections, of
0.5 · 0.5 · 0.015 cm dimension, that were cut from a wood sample of 0.5 · 0.5 · 0.5 cm initially, then
the larger wood sample was stained by acid fuchsin, from which two 5-mg wood cross-sections (that
adjoined the three 5-mg wood cross-sections, mentioned above) were cut. Nematode-staining-spots
(NSSs) in each of the two stained sections were counted under a microscope at 100· magnification. If
there were eight or more NSSs present, the adjoining three sections were used for PCR assays. The
B. xylophilus – specific amplicon of 403 bp (DQ855275) was generated by PCR assay from 100.00% of
5-mg wood cross-sections that contained more than eight Bx NSSs by the PCR assay. The entire
sampling procedure took only 10 min indicating that it is suitable for the fast estimation of nematode
numbers in the wood of P. massonina as the prelimary sample selections for other more expensive
Bx-detection methods such as PCR assay
Phonon self-energy corrections to non-zero wavevector phonon modes in single-layer graphene
Phonon self-energy corrections have mostly been studied theoretically and
experimentally for phonon modes with zone-center (q = 0) wave-vectors. Here,
gate-modulated Raman scattering is used to study phonons of a single layer of
graphene (1LG) in the frequency range from 2350 to 2750 cm-1, which shows the
G* and the G'-band features originating from a double-resonant Raman process
with q \not= 0. The observed phonon renormalization effects are different from
what is observed for the zone-center q = 0 case. To explain our experimental
findings, we explored the phonon self-energy for the phonons with non-zero
wave-vectors (q \not= 0) in 1LG in which the frequencies and decay widths are
expected to behave oppositely to the behavior observed in the corresponding
zone-center q = 0 processes. Within this framework, we resolve the
identification of the phonon modes contributing to the G* Raman feature at 2450
cm-1 to include the iTO+LA combination modes with q \not= 0 and the 2iTO
overtone modes with q = 0, showing both to be associated with wave-vectors near
the high symmetry point K in the Brillouin zone
Evolution equations of curvature tensors along the hyperbolic geometric flow
We consider the hyperbolic geometric flow introduced by Kong and Liu [KL]. When the Riemannian
metric evolve, then so does its curvature. Using the techniques and ideas of
S.Brendle [Br,BS], we derive evolution equations for the Levi-Civita connection
and the curvature tensors along the hyperbolic geometric flow. The method and
results are computed and written in global tensor form, different from the
local normal coordinate method in [DKL1]. In addition, we further show that any
solution to the hyperbolic geometric flow that develops a singularity in finite
time has unbounded Ricci curvature.Comment: 15 page
Continuous volumetric imaging via an optical phase-locked ultrasound lens
In vivo imaging at high spatiotemporal resolution is key to the understanding of complex biological systems. We integrated an optical phase-locked ultrasound lens into a two-photon fluorescence microscope and achieved microsecond-scale axial scanning, thus enabling volumetric imaging at tens of hertz. We applied this system to multicolor volumetric imaging of processes sensitive to motion artifacts, including calcium dynamics in behaving mouse brain and transient morphology changes and trafficking of immune cells
Discovery of Radio/X-ray/Optical Resolved Supernova Remnants in the Center of the Andromeda Galaxy
We have detected a spatially resolved supernova remnant (SNR) in the center
of the Andromeda Galaxy, in radio, X-ray, and optical wavelengths. These
observations provide the highest spatial resolution imaging of a
radio/X-ray/optical SNR in that galaxy to date. The multi-wavelength
morphology, radio spectral index, X-ray colors, and narrow-band optical imaging
are consistent with a shell-type SNR. A second SNR is also seen resolved in
both radio and X-ray. By comparing the morphological sturcture of the SNRs in
different wavelengths and with that in our own Galaxy, we can study the shock
morphologies of SNRs in the Andromeda Galaxy. The proximity of the SNRs to the
core suggests high interstellar medium density in the vicinity of the SNRs in
the center of the Andromeda Galaxy.Comment: 5 pages, 3 figures, accepted for publication in ApJ
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