Cr3+-green common opal from Turnali, North-eastern Turkey

Poste

Lead-arsenic soil geochemical study as an exploration guide over the Killik volcanogenic massive sulfide deposit, Northeastern Turkey

WOS: 000230576800003A mountainous terrain, the eastern Pontide tectonic belt, located in northeastern Turkey, contains more than 60 known volcanogenic massive sulfide (VMS) deposits that differ in reserves (0.1-30 million tonnes) and grades. Soil geochemistry is conventionally used in exploration programs to discover concealed VMS deposits in the region. In the present study, Pb and As element pair were used as pathfinder elements to investigate the relationship of their anomalies to a completely delineated ore deposit (Killik VMS deposit) in an orientation survey that served as a natural physical model. Two hundred forty soil samples were analyzed in the present study. The two elements, which represent the opposite ends of the mobility range, revealed high contrast and overlapped each other at the location of the ore deposit due to enhancement of the anomalies by hydromorphic dispersion, which is an indication that soil samples would produce reliable results. The successful delineation of the deposit is remarkable considering the rough topography and the climatic limitations. Previously the extremely moist and temperate climate was thought to cause excessive leaching of the trace element pathfinders from the ore deposits to produce extensive anomalies usually extending away from the mineralization thus, leading to erroneous results and/or extensive anomalous areas. But the present research has shown that the method can be used effectively if the sampling and data evaluation is carefully conducted. (C)\ 2005 Elsevier B.V. All rights reserved

Determination of mass attenuation coefficients for natural minerals from different places of Turkey

The mass attenuation coefficients (?m) for some natural minerals taken from different places of Turkey were measured at various low photon energies. The Am-241 radioactive source and Zr and Cd secondary exciter are used to get photons in the energy range 15.8 to 26.2 keV. For each sample and energy, Io and I intensities which are without and after attenuation were measured by a Si(Li) detector coupled to multi channel analyzer (MCA) using narrow beam transmission arrangement and mass attenuation coefficients were determined using these intensities. ©2011 Academic Journals

Cr3+-green common opal from Turnali, North-eastern Turkey

Poste

Investigation of some biological active properties of Anzer bee-pollen

41st FEBS Congress on Molecular and Systems Biology for a Better Life -- SEP 03-08, 2016 -- Kusadasi, TURKEYWOS: 000383616901875[No abstract available]FEB

Expression stability of six housekeeping genes: a proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR

Constantly expressed genes are used as internal controls in relative quantification studies. Suitable internal controls for such studies have not yet been defined for Pseudomonas aeruginosa. In this study, the genes ampC, fabD, proC, pbp-2, rpoD and rpoS of P. aeruginosa were compared in terms of expression stability by real-time quantitative RT-PCR. A total of 23 strains with diverse resistance phenotypes were studied. Stability of expression among the housekeeping genes was assessed on the basis of correlation coefficients, with the best-correlated pair accepted as being the most stable one. Eventually, proC and rpoD formed the most stable pair (r = 0.958; P < 0.001). Next, in four ciprofloxacin-selected nfxC-like mutants, levels of oprD, oprM and oprN mRNA were compared with those of their wild-type counterparts. The comparison was made after correcting the raw values by the geometric mean of the internal control genes proC and rpoD. The level of oprN mRNA was significantly up-regulated, while the oprD gene was down-regulated (although this difference was statistically insignificant), in the mutants. This expression pattern was consistent with that of the expected expression profile of nfxC-type mutants; this experiment therefore ends further support to the use of proC and rpoD genes simultaneously as internal controls for such studies

Anti-inflammatory activities of some bee products by inhibition of bovine testes hyaluronidase

Background: Apitherapeutic products contain many valuable bioactive compounds which are responsible for biologically active features such as anti-oxidant, anti-viral, anti-microbial and anti-inflammatory properties. Objective: This study investigated in vitro anti-inflammatory potentials in terms of hyaluronidase inhibition of three different bee products, propolis, pollens and royal jellies. Methods: Water soluble extracts of the samples were prepared, and inhibitions of bovine serum hyaluronidase were used. Inhibitions were expressed as percentages of inhibition (IC50;?g/mL or mg/mL) causing 50% inhibition of the enzyme. Results: All the bee products exhibited anti-hyaluronidase activities in the order propolis, pollen, and finally royal jelly. Inhibition was also correlated with the samples’ total phenolic contents. Conclusion: The study results confirm that water-soluble propolis is the best inhibitory agent among the bee products, and that these may be used as anti-inflammatory agents. However, further in vivo studies are now needed to clarify their pharmaceutical potentials as medicines. © 2016 Bentham Science Publishers

Evaluation of phenolic compounds in Tilia rubra Subsp. caucasica by HPLC-UV and HPLC-UV-MS/MS

Profile of phenolic compounds of Tilia rubra subsp. caucasica was measured by high performance liquid chromatography coupled with ultraviolet and tandem mass spectroscopy. Three different extraction methods (methonolic, selective extraction, and acidic hydrolysis) were used to evaluate phenolic composition and antioxidant capacity in three different parts of T. rubra. The antioxidant activities of the species were investigated in terms of total phenolics and flavonoids, and cupric reducing antioxidant capacity and 1,1-diphenyl-2- picrylhydrazyl scavenging assays. Different phenolic compounds related to antioxidant activities of three different parts and three different extraction ways of T. rubra were determined by high performance liquid chromatography- ultraviolet and high performance liquid chromatography-mass spectroscopy. Gallic and protocatechuic acid were the main phenolic compounds in the all extracts and parts of Tilia rubra subsp. caucasica by high performance liquid chromatography-ultraviolet ranging from 356.20 to 159.83 and 1873.90 to 720.80 ?g phenolic compound/g dry sample, respectively. Epicatechin, luteolin, and rhamnazin were detected by high performance liquid chromatography-mass spectroscopy. © 2014 Copyright Taylor and Francis Group, LLC.Huseyin S¸ahin would like to thank TUB?TAK B?DEB for the financial support given to him. The authors would also like to thank Professor Salih Terziog?lu for identifying the tilia genus and Faculty of Pharmacology, Karadeniz Technical University for helping with the HPLC device

Effect of carbapenems on the transcriptional expression of the oprD, oprM and oprN genes in Pseudomonas aeruginosa

The effects of imipenem and meropenem on the transcriptional expression of resistance-related genes oprD, oprM and oprN in Pseudomonas aeruginosa were studied by quantitative real-time PCR. Four strains were examined: the type strain PT5 (PAO1), its derivatives M7 and PT149, and a clinical isolate, PaKT3. The derivative M7 is a nalB mutant, overexpressing the MexAB-OprM pump, and the derivative PT149 is a nfxC-type mutant, overexpressing the MexEF-OprN pump while it is down-regulated for the OprD protein. After 18 h incubation in broth, the cultures were divided into three portions. Two were supplemented with antibiotics and the other was left antibiotic-free as the control. After a further 45 min incubation, total RNA was isolated from the strains by guanidine denaturation and acid-phenol/chloroform extraction. DNA-free total RNAs were immediately reverse-transcribed by MMuLV reverse transcriptase. Concentrations of mRNAs obtained by quantitative PCR were expressed relative to uninduced portions of the strains. The results showed that oprD was relatively stable against carbapenem antibiotics. oprM was induced significantly by imipenem in only one strain and oprN was induced by imipenem in most of the strains. The responses at the mRNA level found here were unexpected and suggested a chaotic, unpredictable regulatory mechanism