An anisotropic distribution of spin vectors in asteroid families

Current amount of ~500 asteroid models derived from the disk-integrated photometry by the lightcurve inversion method allows us to study not only the spin-vector properties of the whole population of MBAs, but also of several individual collisional families. We create a data set of 152 asteroids that were identified by the HCM method as members of ten collisional families, among them are 31 newly derived unique models and 24 new models with well-constrained pole-ecliptic latitudes of the spin axes. The remaining models are adopted from the DAMIT database or the literature. We revise the preliminary family membership identification by the HCM method according to several additional criteria - taxonomic type, color, albedo, maximum Yarkovsky semi-major axis drift and the consistency with the size-frequency distribution of each family, and consequently we remove interlopers. We then present the spin-vector distributions for eight asteroidal families. We use a combined orbital- and spin-evolution model to explain the observed spin-vector properties of objects among collisional families. In general, we observe for studied families similar trends in the (a_p, \beta) space: (i) larger asteroids are situated in the proximity of the center of the family; (ii) asteroids with \beta>0{\deg} are usually found to the right from the family center; (iii) on the other hand, asteroids with \beta<0{\deg} to the left from the center; (iv) majority of asteroids have large pole-ecliptic latitudes (|\beta|\gtrsim 30{\deg}); and finally (v) some families have a statistically significant excess of asteroids with \beta>0{\deg} or \beta<0{\deg}. Our numerical simulation of the long-term evolution of a collisional family is capable of reproducing well the observed spin-vector properties. Using this simulation, we also independently constrain the age of families Flora (1.0\pm0.5 Gyr) and Koronis (2.5-4 Gyr).Comment: Accepted for publication in A&A (September 16, 2013

The cell cycle regulator p27kip1 contributes to growth and differentiation of osteoblasts

The cyclin-dependent kinase (cdk) inhibitors are key regulators of cell cycle progression. p27 and p21 are members of the Cip/Kip family of cdk inhibitors and regulate cell growth by inactivating cell cycle stage-specific CDK-cyclin complexes. Because down-regulation of osteoprogenitor proliferation is a critical step for osteoblast differentiation, we investigated expression of p27 and p21 during development of the osteoblast phenotype in rat calvarial osteoblasts and in proliferating and growth-inhibited osteosarcoma ROS 17/2.8 cells. Expression of these proteins indicates that p21, which predominates in the growth period, is related to proliferation control. p27 levels are maximal postproliferatively, suggesting a role in the transition from cell proliferation to osteoblast differentiation. We directly examined the role of p27 during differentiation of osteoprogenitor cells derived from the bone marrow (BM) of p27-/- mice. BM cells from p27 null mice exhibited increased proliferative activity compared with BM cells from wild-type mice and formed an increased number and larger size of osteoblastic colonies, which further differentiated to the mineralization stage. Although p27-/- adherent marrow cells proliferate faster, they retain competency for differentiation, which may result, in part, from observed higher p21 levels compared with wild type. Histological studies of p27-/- bones also showed an increased cellularity in the marrow cavity compared with the p27+/+. The increased proliferation in bone does not lead to tumorigenesis, in contrast to observed adenomas in the null mice. Taken together, these findings indicate that p27 plays a key role in regulating osteoblast differentiation by controlling proliferation-related events in bone cells

Design of Cationic Multi-Walled Carbon Nanotubes as Efficient siRNA Vectors for Lung Cancer Xenograft Eradication

Polo-Like Kinase (PLK1) has been identified as a potential target in cancer gene therapy via chemical or genetic inhibitory approaches. The biomedical applications of chemically functionalized carbon nanotubes (f-CNTs) in cancer therapy have been studied due to their ability to efficiently deliver siRNA intracellularly. In this study, we established the capacity of cationic MWNT-NH3+ to deliver the apoptotic siRNA against PLK1 (siPLK1) in Calu6 tumor xenografts by direct intratumoural injections. A direct comparison with cationic liposomes was made. This study validates the PLK1 gene as a potential target in cancer gene therapy including lung cancer, as demonstrated by the therapeutic efficacy of siPLK1:MWNT-NH3+ complexes and their ability to significantly improve animal survival. Biological analysis of the siPLK1:MWNT-NH3+ treated tumors by RT-PCR and Western blot, in addition to TUNEL staining confirmed the biological functionality of the siRNA intratumourally, suggesting that tumor eradication was due to PLK1 knockdown. Furthermore, by using a fluorescently labelled, non-coding siRNA sequence complexed with MWNT-NH3+, we established for the first time that the improved therapeutic efficacy observed in f-CNT-based siRNA delivery is directly proportional to the enhanced siRNA retention in the solid tumor and subsequent uptake by tumor cells after local administration in vivo

Supernovae 2016bdu and 2005gl, and their link with SN 2009ip-like transients: another piece of the puzzle

Supernova (SN) 2016bdu is an unusual transient resembling SN 2009ip. SN 2009ip-like events are characterized by a long-lasting phase of erratic variability which ends with two luminous outbursts a few weeks apart. The second outburst is significantly more luminous (about 3 mag) than the first. In the case of SN 2016bdu, the first outburst (Event A) reached an absolute magnitude M(r) ~ -15.3 mag, while the second one (Event B) occurred over one month later and reached M(r) ~ -18 mag. By inspecting archival data, a faint source at the position of SN 2016bdu is detectable several times in the past few years. We interpret these detections as signatures of a phase of erratic variability, similar to that experienced by SN 2009ip between 2008 and mid-2012, and resembling the currently observed variability of the luminous blue variable SN 2000ch in NGC 3432. Spectroscopic monitoring of SN 2016bdu during the second peak initially shows features typical of a SN IIn. One month after the Event B maximum, the spectra develop broad Balmer lines with P Cygni profiles and broad metal features. At these late phases, the spectra resemble those of a typical Type II SN. All members of this SN 2009ip-like group are remarkably similar to the Type IIn SN 2005gl. For this object, the claim of a terminal SN explosion is supported by the disappearance of the progenitor star. The similarity with SN 2005gl suggests that all members of this family may finally explode as genuine SNe, although the unequivocal detection of nucleosynthesised elements in their nebular spectra is still missing.Comment: Submitted to MNRAS on April 10, 2017; re-submitted on June 23 including suggestions from the referee. 24 pages, 12 figures, 5 table

A comparative HST imaging study of the host galaxies of radio-quiet quasars, radio-loud quasars and radio galaxies: Paper I

We present the first results from a major HST WFPC2 imaging study aimed at providing the first statistically meaningful comparison of the morphologies, luminosities, scalelengths and colours of the host galaxies of radio-quiet quasars, radio-loud quasars, and radio galaxies. We describe the design of this study and present the images which have been obtained for the first half of our 33-source sample. We find that the hosts of all three classes of luminous AGN are massive elliptical galaxies, with scalelengths ~=10 kpc, and R-K colours consistent with mature stellar populations. Most importantly this is the the first unambiguous evidence that, just like radio-loud quasars, essentially all radio-quiet quasars brighter than M_R = -24 reside in massive ellipticals. This result removes the possibility that radio `loudness' is directly linked to host galaxy morphology, but is however in excellent accord with the black-hole/spheroid mass correlation recently highlighted by Magorrian et al. (1998). We apply the relations given by Magorrian et al. to infer the expected Eddington luminosity of the putative black hole at the centre of each of the spheroidal host galaxies we have uncovered. Comparison with the actual nuclear R-band luminosities suggests that the black holes in most of these galaxies are radiating at a few percent of the Eddington luminosity; the brightest host galaxies in our low-z sample are capable of hosting quasars with M_R = -28, comparable to the most luminous quasars at z = 3. Finally we discuss our host-derived black-hole masses in the context of the radio-luminosity:black-hole mass correlation recently uncovered for nearby galaxies by Franceschini et al. (1998), and the resulting implications for the physical origin of radio loudness.Comment: Submitted for publication in the Astrophysical Journal, 55 pages of latex, plus 12 postscript figures (Figures 1a-1s (greyscales of images and model fits, and Figures 2a-2g (luminosity profiles and model fits) can be downloaded from http://www.roe.ac.uk/astronomy/html/rjm1.shtml

Asteroids' physical models from combined dense and sparse photometry and scaling of the YORP effect by the observed obliquity distribution

The larger number of models of asteroid shapes and their rotational states derived by the lightcurve inversion give us better insight into both the nature of individual objects and the whole asteroid population. With a larger statistical sample we can study the physical properties of asteroid populations, such as main-belt asteroids or individual asteroid families, in more detail. Shape models can also be used in combination with other types of observational data (IR, adaptive optics images, stellar occultations), e.g., to determine sizes and thermal properties. We use all available photometric data of asteroids to derive their physical models by the lightcurve inversion method and compare the observed pole latitude distributions of all asteroids with known convex shape models with the simulated pole latitude distributions. We used classical dense photometric lightcurves from several sources and sparse-in-time photometry from the U.S. Naval Observatory in Flagstaff, Catalina Sky Survey, and La Palma surveys (IAU codes 689, 703, 950) in the lightcurve inversion method to determine asteroid convex models and their rotational states. We also extended a simple dynamical model for the spin evolution of asteroids used in our previous paper. We present 119 new asteroid models derived from combined dense and sparse-in-time photometry. We discuss the reliability of asteroid shape models derived only from Catalina Sky Survey data (IAU code 703) and present 20 such models. By using different values for a scaling parameter cYORP (corresponds to the magnitude of the YORP momentum) in the dynamical model for the spin evolution and by comparing synthetics and observed pole-latitude distributions, we were able to constrain the typical values of the cYORP parameter as between 0.05 and 0.6.Comment: Accepted for publication in A&A, January 15, 201