Transport of Phosphate by Renal Brush Border Membrane Vesicle (BBMV) during Development - Role of the Growth Hormone-

It is well documented that plasma concentrations of Pi (inorganic phosphorus) are higher in developing subjects than in adults. In a previous study, we demonstrated that the Vmax (capacity) of the Na-Pi cotransport mechanism of the renal brush border membrane vesicles was higher in immature than mature rats. In this study, we evaluated the role of a growth hormone in the maintenance of a higher Vmax observed in immature rats. In mature rats, serum Pi, and the tubular reabsorption of Pi (TRPi) increased in the growth hormone treated animals. On the other hand, those values were not changed by growth hormone treatment in immature rats. In kinetic analysis, the Km (affinity) values were not different between the control (growth hormone-untreated) and growth hormone-treated renal brush border membrane vesicles in both immature and mature rats. The Vmax of the immature rats also was not changed by growth hormone treatment. On the contrary, Vmax increased significantly in the growth-hormone treated than the control mature rats. With the above findings, it seems that immature rats reabsorb Pi maximally even in the control state, and it is likely that a growth hormone is responsible for the phenomenon

The Pathogenetic Role of Reactive Oxygen Species in Aminonucleoside Nephrosis

We studied the pathogenetic role of reactive oxygen species (ROS) in rats with puromycin aminonucleoside nephrosis (PAN). Heavy albuminuria with markedly decreased density of the anionic sites (AS) on glomerular basement membrane (GBM) (2. 6 ± O. 98 compared to 20. 0 ± 1. 61 AS/l,OOOnm GBM in control) developed 7 days after PA injection. The malondialdehyde (MDA) levels in kidney homogenates increased gradually (1. 16 ± O. 18 at day -1 to 1. 97 ± O. 23/g protein at day 5). While catalase or dimethyl sulfoxide, administered with PA, did not affect the course of PAN. superoxide dismutase and allopurinol reduced proteinuria and decreased loss of the AS (11. 7 ± 2. 80 and 13, 7 ± 1. 27 AS/l.000nm GBM, reo spectively) at day 7. These findings suggest that proteinuria in PAN results from the loss of GBM AS. in which ROS generated by xanthine oxidase system plays an import. ant role

A Simple Sink Mobility Support Algorithm for Routing Protocols in Wireless Sensor Networks

Abstract. In order to support the sink mobility of conventional routing protocols, we propose a simple route maintaining algorithm which does not use the flooding method. In the proposed method, when the sink loses the connection with the source, it does not rebuild an entire route but simply repairs the existing route based on local information. Experimental results show that the proposed algorithm drastically improves the conventional routing protocols in terms of both energy and delay in case of mobile sink

Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103(+)CD11b(+) Dendritic Cells

Retinal dehydrogenase (RALDH) enzymatic activities catalyze the conversion of vitamin A to its metabolite Retinoic acid (RA) in intestinal dendritic cells (DCs) and promote immunological tolerance. However, precise understanding of the exogenous factors that act as initial trigger of RALDH activity in these cells is still evolving. By using germ-free (GF) mice raised on an antigen free (AF) elemental diet, we find that certain components in diet are critically required to establish optimal RALDH expression and activity, most prominently in small intestinal CD103(+)CD11b(+) DCs (siLP-DCs) right from the beginning of their lives. Surprisingly, systematic screens using modified diets devoid of individual dietary components indicate that proteins, starch and minerals are dispensable for this activity. On the other hand, in depth comparison between subtle differences in dietary composition among different dietary regimes reveal that adequate glucose concentration in diet is a critical determinant for establishing RALDH activity specifically in siLP-DCs. Consequently, pre-treatment of siLP-DCs, and not mesenteric lymph node derived MLNDCs with glucose, results in significant enhancement in the in vitro generation of induced Regulatory T (iTreg) cells. Our findings reveal previously underappreciated role of dietary glucose concentration in establishing regulatory properties in intestinal DCs, thereby extending a potential therapeutic module against intestinal inflammation11Ysciescopu

Short-Term Efficacy of Enzyme Replacement Therapy in Korean Patients with Fabry Disease

Fabrazyme has been widely used for treatment of Fabry disease since its approval by the U.S. Food and Drug Administration in 2003. This study was undertaken to assess the short-term efficacy and safety of enzyme replacement therapy (ERT) for Fabry disease in Korea. Eight male patients and three female symptomatic carriers aged 13 to 48 yr were included. Fabrazyme was administered by intravenous infusion at a dose of 1 mg/kg every 2 weeks. Plasma and urine globotriaosylceramide (GL-3) levels, serum creatinine, creatinine clearance, and 24-hr urine protein levels were measured every 3 months. Kidney biopsies, ophthalmologic exams, and pure tone audiometry were performed before and 1 yr after ERT. Kidney function, including serum creatinine, creatinine clearance, and the 24-hr urine protein level, remained stable during ERT. Plasma and urine GL-3 levels were reduced within 3 to 6 months of ERT initiation. Microvascular endothelial deposits of GL-3 were decreased from renal biopsy specimens after 1 yr of treatment. The severity of sensorineural hearing loss and tinnitus did not improve after ERT. ERT is safe and effective in stabilizing renal function and clearing microvascular endothelial GL-3 from kidney biopsy specimen in Korean patients with Fabry disease

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

신장의 Brush Border Membrane Vesicle (BBMV)을 통한 무기인 이동의 발육에 따른 변화

To elucidate the mechanism for higher tubular reabsorption of Pi (inorganic phosphate) observed in the young growing animals, vesicle preparations of proximal tubule brush border obtained from male Wistar rats of either 4-5 weeks (young) or 9-10 weeks (more mature) of age were used to assess the kinetic characteristics of the Na-Pi cotransporter. No significant difference was noted in the affinity (Km) of this transport system, but the capacity (Vmax) was about 2 times higher in the young than the more mature rat brush border membrane vesicles (BBMV). The results demonstrate that renal adaptation in Pi transport in the proximal tubule brush border membrane during early developmental period is mainly due to higher capacity of the Na-Pi cotransport carrier. However, physical forces along the proximal tubule brush border membrane and extrinsic factors such as growth hormone and parathyroid hormone may have additional role in the maintenance of positive Pi balance during the early developmental period in vivo

백혈구접착억제의 종양항원 특이성 및 반복백혈구접학억제 검사에 관한 연구

The tube Leukocyte Adherence Inhibition (LA!) assays were performed to study immunological reactivity of patients with a variety of tumors (lung cancer, cervix cancer, stomach cancer and hepatoma). The result showed that leukocytes from cancer patients reacted only to the antigenic extracts of tumors of the same type and that the LAI response was directed to an organ type specific neoantigen. After the initial tube LAI assay with normal lung tissue, the repeated tube LAI assay was performed with lung cancer extracts on the resultant cells adherent to the glass tube. In 8 patients with lung cencer, the changes in the number of nonadherent cells were all above 100%, while in 9 healthy control subjects they were all below 100%. NAIs (non adherence index) of the repeated tube LAI assay were significantly higher than those of the original tube LAI assay in patients with lung cancer (p < 0.01)