cDNA cloning and inducible expression of human multidrug resistance associated protein 3 (MRP3)1The sequence reported in this paper has been submitted to GenBank with the accession number of AB010887.1

AbstractPreviously, we cloned rat MRP3 as a candidate for an inducible transporter for the biliary excretion of organic anions [Hirohashi et al. (1998) Mol. Pharmacol. 53, 1068–1075]. In the present study, we cloned human MRP3 (1527 amino acids) from Caco-2 cells. Human MRP3 is predominantly expressed in liver, small intestine and colon; hepatic expression of MRP3 was observed in humans but not in normal rats. In HepG2 cells, the expression of MRP3 was induced by phenobarbital. These results suggest that MRP3 may act as an inducible transporter in the biliary and intestinal excretion of organic anions

IOLmodeling and light imulation for glare source analysis

眼病として眼球の水晶体が白く濁る白内障がある.この病気により一度白く濁った水晶体は元に戻ることは無い.水晶体中の白く濁った部分を除去し,その中に眼内レンズを挿入する小切開白内障手術を行うことにより,視力の回復が見込まれる.しかし,術後の後遺症の一つとしてグレアの知覚が挙げられる.グレアとはレンズを挿入したことにより生じるぎらつきのことを指す.グレアの発生原因に関する詳しい解析はまだ十分に行われていない.本稿では,グレア発生原因解析の基礎研究として,眼内レンズ断面形状のモデル化.眼内レンズに入射する光を反射・屈折を考慮して光線追跡を行い,眼底での輝度分布をシミュレーションする方法について述べる.Cataract is the eye disease which clouds the lens of the eye. Vision can be returned through the cataract operation which inserts an intraocular lens into the eye. However a postoperative after effect exists in which a glare is generated. In this paper we approximate IOL's cross section shape using least squares approach, and trace light rays via taking into accout reflection and refraction. Then by observing the distribution of Rays onto a Fundus model we determined a glare exists

Relationship between medical examination methods and actual conditions of life for preserving visual acuity in primary and junior high school: Verification of the current vision test

The deterioration of children’s eyesight is on the rise. Children's lives have changed in a short period of time, such as an increase in blue light due to the use of electronic devices. It is necessary to consider non-traditional content of health guidance for prevention. The accurate visual acuity test is important to examine the effects of visual acuity and environmental or living factors. We examined the accuracy of the visual acuity test with the three stage Landolt ring for children from the relationship with the eye axial length. After performing a normal detailed visual acuity test of the children and the correlation analysis with the eye axial length, we examined the distribution of the eye axis according to the results of the three-stage visual acuity test by the Landolt ring. The number of children measured eye axial length was 13. The correlation coefficient between detailed visual acuity test and eye axial length was negative. According to the results of the three-stage visual acuity test, the distribution of the eye axis is good for the right eye: the average eye axis of “A” was 23.44 mm, moderate: the average of “B” was 22.58 mm. The average of the left eye axis for “A” children is 23.34 mm, for “B” is 23.08 mm, and for “C” is 23.7 mm. The correlation coefficient between the detailed visual acuity test and the eye axial length was negative on both sides, namely a negative correlation between the visual acuity and the eye axial length. There was a possibility of not correspondence between the three-stage visual test and the eye axial length. In the case of children, it was considered that their visual performance and answers were different because of their mood and physical condition on the day

IOLmodeling and light imulation for glare source analysis

MIRU2007 第10回 画像の認識・理解シンポジウム ポスター資料 ; 開催日時:2007年7月30日～8月1

A method for accurate reconstruction of fundus volume data from radially cross-sectional images

医学の分野において,眼病の早期発見および経過観察のために,眼底の3次元形状の詳細な観察が望まれている.本論文では,Optical Coherence Tomography (OCT)を用いて,放射状にスキャンされた複数枚の断面画像から,眼底ボリュームデータを高精度に構築する手法を提案する.すなわち,眼球の動きによる回転誤差を考慮して,スキャン時に生じるスキャン位置のずれを合わせる手法を開発した.この手法を用いて構築した眼底ボリュームデータに可視化処理を施すことにより,眼底表面形状とともに,眼底内部構造も観察が可能となり,医師の診断支援になることが期待される.In medicine, detail observation of a three-dimensional fundus shape is necessary for diagnosing eye-disease accurately and checking the treatment process. In the paper, we propose a method for accurate reconstruction fundus volume data from cross-sectional images scanned radially with an optical coherence tomography (OCT). We developed the method for correct deviation from positions of cross-sectional images taking into account errors caused by eye movement. Visualizing the fundus volume data generated by the proposed method, the fundus shape and the internal structures can be easily observed. The method is a promising technique for diagnosis of eye-disease

A method for accurate reconstruction of fundus volume data from radially cross-sectional images

MIRU 2007 第10回 画像の認識・理解シンポジウム ポスター資料 ; 開催場所 : 広島市立大学, 広島 ; 開催日時:2007年7月30日～8月1

Effects of ferric citrate on intracellular oxidative stress markers after hydrogen peroxide treatment of human U937 monocytes

Phosphate binders, such as iron （III） citrate hydrate （FCH）, are essential medications for hemodialysis patients. Some in vivo studies have demonstrated that FCH prevented induction of oxidative stress in the presence of transferrin. However, how FCH affects iron-related oxidative stress in the absence of transferrin remains unclear. In the current study, we investigated the effects of ferric citrate （FC） on oxidative stress in the absence of transferrin in vitro to address this question. Human U937 monocytes were pretreated with FC, iron （II） chloride tetrahydrate （FeCl2・4H2O）, iron （III） chloride hexahydrate （FeCl3・6H2O）, or saccharated ferric oxide for 24 h and then treated with 10-mM hydrogen peroxide （H2O2） for 30 min. The final Fe concentrations were adjusted to approximately 200µg/dl. Iron concentration, intracellular reactive oxygen species （ROS） levels, and intracellular lipid peroxidation of the cell membrane were measured. After treatment with FC, iron concentration and ROS levels increased. Change in lipid peroxidation after treatment with FC was not observed. However, after treatment with H2O2, no change was observed in the intracellular ROS levels in FC-pretreated cells, whereas lipid peroxidation of the cell membrane was decreased. Despite the high iron concentration in FC-pretreated cells, neither intracellular ROS nor cell membrane lipid peroxidation levels were increased with H2O2 treatment. Their results might represent antioxidative effects of FC. The results of this study may contribute to a better understanding of the effects of oxidative stress in hemodialysis patients treated with FCH