Use of genetic methods for determining patterns and processes during marine biological invasions

Invasive species are widely recognised as one of the major threats to marine biodiversity worldwide. With increasingly faster and more frequent transoceanic shipping, propagule pressure in the marine environment is likely to further increase, leading to a need for effective strategies for the early detection, prevention and control of marine invasive species. However, such strategies are often difficult to implement as many marine species cannot be accurately categorised as either native or non-native. For these reasons molecular genetic methods have increasingly been utilised for the study of marine invasive species. The potential for molecular data to enhance traditional morphology-based information is recognised and there has been huge progress in the application of molecular genetic methods to the study of marine bioinvasions in the last two decades. This work aims to build on and extend current advances in the use of molecular techniques for understanding marine bioinvasions, and in particular invasive ascidians. Part One (Chapters Two and Three) contains chapters that focus on the detection and identification of marine invasive species with molecular methods. Part Two (Chapters Four, Five and Six) examines how molecular methods can aid in understanding marine invasions and the affect of genetic diversity on invasive populations. The research in this thesis demonstrates the usefulness of molecular genetics for marine invasive species research. Chapter Two describes the development of a highly sensitive assay for rapid and accurate identification of an invasive clam from environmental samples and has the ability to enhance current marine pest surveillance methods. Chapter Three demonstrates the power of molecular methods for invasive species identification. To effectively monitor and / or control the ongoing anthropogenic spread of invasive marine species there is also a need for extensive molecular inventories of the extant marine invertebrate biodiversity. An understanding of the genetic diversity of invasive species populations is also required and will increase understanding of the species biology and lead to more effective management strategies. Chapters Four and Five highlight the applications of molecular genetic methods for identifying the invasive species / variants present and elucidating particular populations that served as the source of an introduction. This information can contribute to effective national and international policies and management strategies. Chapters Five and Six also show how molecular genetic data can aid in understanding why some species are invasive. The results from these two chapters add to growing evidence that, for colonial organisms, reductions in population level genetic diversity may alter colony interaction dynamics and enhance the invasive potential of newly colonising species. In the future, invasive species research will increasingly utilise metagenomics / next-generation sequencing (NGS) technologies. Such applications will provide the ability to not only detect specific invasive species but also the native species present in a sample to assess environmental health. Studies of invasive species are increasingly using functional markers to identify physiologically and ecologically important traits. Future gene expression experiments can utilise NGS technology to identify the genes involved in producing invasive phenotypes and species. Such studies may provide findings which could be of both evolutionary and importantly, practical interest for guiding invasive species management decisions. The application of molecular genetic methods to understanding the biology of invasive species is an extremely promising area of research and such knowledge should be utilised to guide and inform management decisions

The microstructure of the student wellbeing process questionnaire

Background: The wellbeing process model formed the basis of questionnaires that can demonstrate which factors predict negative and positive wellbeing outcomes. The Student Wellbeing Process Questionnaire (Student WPQ) uses stressor, negative coping, psychological capital and social support scales to predict positive and negative wellbeing outcomes. Aims: The usual method of scoring the WPQ has been to sum relevant questions in each scale. The aim of the present analyses was to investigate the microstructure of the WPQ and examine the profile of individual predictor and outcome items. Methodology: The research was approved by the ethics committee, School of Psychology, Cardiff University, and carried out with the informed consent of the volunteers (1481 psychology undergraduates; 89.4% female; 49.7% year 1; mean age 19.5 years). An online survey was carried out, and a MANOVA was conducted to examine associations between the wellbeing process predictor variables and the wellbeing outcomes. Results: A multivariate analysis of variance showed that the majority of individual predictors had significant overall effects. Some of the predictors (optimism; self-esteem, developmental challenges; time pressure; avoidance coping) had significant effects on all outcomes, which explains the global effects of the positive personality and stressor composite variables. Negative coping variables had significant effects on all negative outcomes. Other variables had selective effects on specific outcome measures. Conclusion: The independent variables from the student wellbeing questionnaire are good predictors of both positive and negative wellbeing outcomes. This is observed when either individual items or composite scores are used in the analysis

Evaluating detection limits of next-generation sequencing for the surveillance and monitoring of international marine pests

Most surveillance programmes for marine invasive species (MIS) require considerable taxonomic expertise, are laborious, and are unable to identify species at larval or juvenile stages. Therefore, marine pests may go undetected at the initial stages of incursions when population densities are low. In this study, we evaluated the ability of the benchtop GS Junior™ 454 pyrosequencing system to detect the presence of MIS in complex sample matrices. An initial in-silico evaluation of the mitochondrial cytochrome c oxidase subunit I (COI) and the nuclear small subunit ribosomal DNA (SSU) genes, found that multiple primer sets (targeting a ca. 400 base pair region) would be required to obtain species level identification within the COI gene. In contrast a single universal primer set was designed to target the V1–V3 region of SSU, allowing simultaneous PCR amplification of a wide taxonomic range of MIS. To evaluate the limits of detection of this method, artificial contrived communities (10 species from 5 taxonomic groups) were created using varying concentrations of known DNA samples and PCR products. Environmental samples (water and sediment) spiked with one or five 160 hr old Asterias amurensis larvae were also examined. Pyrosequencing was able to recover DNA/PCR products of individual species present at greater than 0.64% abundance from all tested contrived communities. Additionally, single A. amurensis larvae were detected from both water and sediment samples despite the co-occurrence of a large array of environmental eukaryotes, indicating an equivalent sensitivity to quantitative PCR. NGS technology has tremendous potential for the early detection of marine invasive species worldwide

Associations between the wellbeing process and academic outcomes

Background: The Student Wellbeing Process Questionnaire (Student WPQ) has been used to identify predictors of both positive and negative wellbeing. These variables can now be used to investigate whether different aspects of the wellbeing process are associated with academic outcomes. Aims: The wellbeing process involves established predictors such as exposure to stressors, negative coping, social support, positive personality, and conscientiousness. The wellbeing outcomes are positive (e.g. happiness, positive affect, and life satisfaction), and negative (e.g. stress, anxiety and depression). The aim was to examine associations between these variables and academic outcomes (Grade Point Average [GPA]; perceived efficiency; perceived course stress, and perceived workload). Methodology: The research described in this paper was carried out with the approval of the ethics committee, School of Psychology, Cardiff University, and the informed consent of the participants (1296 psychology undergraduates; 89.4% female; 49.7% year 1; mean age 19.5 years). An online survey was carried out and this included the Student WPQ and academic outcomes. A MANOVA was conducted to examine associations between the wellbeing process variables and the academic outcomes. Results: The main factor associated with the academic outcomes was conscientiousness. Those in the high conscientiousness category had higher GPA sores, reported greater efficiency and higher course stress and workload. GPA scores were also associated with student stressors, with those in the high stress category having lower GPA scores. Greater efficiency was associated with higher scores for positive wellbeing and social support, and lower negative coping. Higher course stress was associated with higher scores for exposure to stressors, negative coping and negative wellbeing. Higher negative wellbeing was also associated with higher perceived workload. Conclusion: Conscientiousness is the best predictor of academic outcomes. Other components of the wellbeing process have selective effects on academic outcomes

Barcoding of the cytochrome oxidase I (COI) indicates a recent introduction of Ciona savignyi into New Zealand and provides a rapid method for Ciona species discrimination

Mitochondrial cytochrome oxidase I (COI) gene sequencing (DNA barcoding) of Ciona specimens from New Zealand (NZ) led to the first record of the solitary ascidian Ciona savignyi in the Southern Hemisphere. We sought to quantify C. savignyi COI genetic diversity around the NZ archipelago and to compare this with diversity within C. savignyi's native range in the north-west Pacific. Ciona savignyi specimens were collected from two NZ sites and from three sites around Japan. COI sequences (595 bp) were amplified and measures of genetic diversity were calculated. Based on differences between their COI sequences we developed a PCR-based assay to distinguish C. savignyi from the morphologically similar C. intestinalis. A total of 12 C. savignyi COI haplotypes were recovered from the 76 samples. Of the four haplotypes observed in NZ, two were unique. From the 10 haplotypes observed in the Japan samples, eight were unique. The C. savignyi populations in Japan were found to contain higher haplotype diversity when compared with those in NZ. The NZ samples contained only a small subset of the haplotype variation of the Japan samples, however, NZ samples did harbor two haplotypes not observed in the Japan samples. A PCR-based assay developed from the COI sequences was able to reliably discriminate the two Ciona species. The low COI genetic diversity within the two NZ C. savignyi populations sampled is consistent with a founder effect associated loss of genetic diversity. The robust PCR-based assay for distinguishing C. savignyi and C. intestinalis may find application in ecological and taxonomic studies and can be applied to both archival materials and live animals

Community Education, Learning and Development : 3rd Edition. Lyn Tett, Edinburgh: Dunedin Academic Press (2010) pp. 126 Pbk. £14.99 ISBN 9781906716103

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Catch it, drop it, leave it there: Writing for Wellbeing as a tool for compassionate practice in Higher Education

This is the story of a series of writing workshops with four undergraduate final year students, in a non-formal, non-graded, non-curriculum space. Students were introduced to ‘writing for wellbeing’ (WfW), using expressive writing strategies adapted from poetry/bibliotherapy practice. Initially intended as a research method for their dissertation projects, the writing workshops evolved into a significant creative space for the students’ own personal development. Shared reflections about our experience of writing together sheds light on the broader potential of WfW as a participatory research method, and as a compassionate approach for writing the self in higher education

Blood, sweat and tears: How did skateboarding's thrasher t-shirt become the fashionista's 'off-duty' look?

While the rise of skateboard companies such as Supreme and Palace have usurped the ranks of fashion’s hierarchy, there remains conflict and tension within the skateboard community regarding the industry’s legitimacy to convey authentic cultural meaning to the images and artefacts pertaining to skateboard lifestyles. Typically characterised as outcasts, lawbreakers and nuisances, skateboarders now signify a visual representation of cool. High fashion brands embody the lifestyle on the pages of fashion magazines, within the frames fashion films and on international catwalks. The profound outcome of this has led many of Skateboarding’s self-identified ‘rebels’ to negotiate the desires of their community alongside the yearnings of aspirational youths determined to authenticate themselves by appropriating the aesthetic and textual lives of skateboarders. This paper presents a discussion on one specific case study, the iconic American skateboard magazine, Thrasher. A dominant voice within the skateboarding community, Thrasher magazine is an anti-establishmentarian, subcultural publication. It shapes a sense of currency within the skateboard community while hinting at the future. Identifying with Skateboarding’s every day practices the magazine emphasises the artistic and visual dissemination of tricks, locations and products, forming a framework of characteristics which examine behaviours, values and social interactions. This paper seeks to describe the history of the magazine’s spirit, identifying the stakeholders who advocate the ‘Thrasher lifestyle’ hypothesising why the magazine’s merchandise became synonymous with the notion of cool. The paper enquires how Thrasher’s t-shirt became a focal point of fascination for the fashion obsessed, addressing some of the tensions felt within the skateboard community on the reporting of this ‘new trend’. The study adopted an ethnographic qualitative approach using mixed methods of non-participant observations, focus groups and in-depth interviews. Primary research in the form of interviews with Thrasher’s publisher, Tony Vitello and editor at large Mike Burnett provided in-depth knowledge of the history of Thrasher. Secondary research including review and analysis of editorial content, interviews and readers’ comments from online publications including Dazed and Confused, GQ style, Hypebeast, and Vogue explored the subject and aided the researches conclusions. Content analysis was used to look for key themes and patterns within the observations, commentary and interviews (Johnson, Onwuegbuzie, Turner 2007). The paper acknowledges the pressures of current fast fashion journalism but asks if there is a need for discussions around commentary on specific areas of subculture fashion, in this case Thrasher Magazine, so as not to alienate those who identify with the appropriated lifestyle

Opening up the black box of a Gateway to Medicine programme : a realist evaluation

Acknowledgements We would like to thank all our study participants for their valuable input into the study and all those who set up and supported the programme. In particular, thank you to Professor Rona Patey, Professor Stephen Davies, Dr Wendy Dollery, Anna Johnston, Dr Christine Kay, Professor Colin Lumsden, Dr Pietro Marini, Dr Sally Middleton, Sarah Miller, Gwen Smith, Laura Young. In addition, we would like to thank Pat Maclennan for all administrative support and Scottish Government for granting the funding. Funding The programme and work was supported by Scottish Goverment.Peer reviewedPublisher PD