9 research outputs found
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Bioactive natural products: facts, applications, and challenges
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Today, there are a strong debate and interest regarding the safety aspects of chemical preservatives addedwidely inmany food products to prevent mainly growth of spoilage and pathogenic microbes. Synthetic compounds are considered responsible for carcinogenic and teratogenic attributes and residual toxicity. To avoid the aforementioned problems, consumers and authorities have increased pressure on food manufacturers to substitute the harmful artificial additives with alternative, more effective, nontoxic, and natural sub-stances. In this context, the use of natural compounds with antimicrobial action presents an intriguing case. Natural antioxidants also demonstrate a wide range of biological and pharmacological activities and are considered to have bene
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Loss of SigB in Listeria monocytogenes strains EGD-e and 10403S leads to hypersensitivity to hydrogen peroxide in stationary phase under aerobic conditions
SigB is the main stress gene regulator in L. monocytogenes affecting the expression of more than 150 genes and thus contributing in multiple stress resistance. Despite its clear role in most stresses, its role in oxidative stress is uncertain as results accompanying the loss of sigB range from hyperresistance to hypersensitivity. Previously, these differences have been attributed to strain variation. In this study, we show conclusively that in contrast to all other stresses, loss of sigB results in hyperresistance against H2O2 (more than 8 log CFU ml-1 compared to the wild type) in aerobically-grown stationary phase cultures of 10403S and EGD-e.. Furthermore, growth at 30°C resulted in higher resistance to oxidative stress than at 37°C. Oxidative stress resistance seemed to be higher with higher levels of oxygen. Under anaerobic conditions, loss of SigB in 10403S did not affect survival against H2O2 while in EGD-e it resulted in a sensitive phenotype. During exponential phase, minor differences occurred as expected due to the absence of sigB transcription. Catalase tests were performed under all conditions and stronger catalase results corresponded well with higher survival underpinning the important role of catalase in this phenotype. Furthermore, we assessed the catalase activity in protein lysates which corresponded with the catalase tests and survival. In addition, RT-PCR showed no differences in transcription between the wild type and the ΔsigB in various oxidative stress genes. Further investigation of the molecular mechanism behind this phenotype and its possible consequences for the overall phenotype of L. monocytogenes are underway
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Divergent evolution of the activity and regulation of the glutamate decarboxylase systems in Listeria monocytogenes EGD-e and 10403S : roles in virulence and acid tolerance
The glutamate decarboxylase (GAD) system has been shown to be important for the survival of Listeria monocytogenes in low pH environments. The bacterium can use this faculty to maintain pH homeostasis under acidic conditions. The accepted model for the GAD system proposes that the antiport of glutamate into the bacterial cell in exchange for γ-aminobutyric acid (GABA) is coupled to an intracellular decarboxylation reaction of glutamate into GABA that consumes protons and therefore facilitates pH homeostasis. Most strains of L. monocytogenes possess three decarboxylase genes (gadD1, D2 & D3) and two antiporter genes (gadT1 & gadT2). Here, we confirm that the gadD3 encodes a glutamate decarboxylase dedicated to the intracellular GAD system (GADi), which produces GABA from cytoplasmic glutamate in the absence of antiport activity. We also compare the functionality of the GAD system between two commonly studied reference strains, EGD-e and 10403S with differences in terms of acid resistance. Through functional genomics we show that EGD-e is unable to export GABA and relies exclusively in the GADi system, which is driven primarily by GadD3 in this strain. In contrast 10403S relies upon GadD2 to maintain both an intracellular and extracellular GAD system (GADi/GADe). Through experiments with a murinised variant of EGD-e (EGDm) in mice, we found that the GAD system plays a significant role in the overall virulence of this strain. Double mutants lacking either gadD1D3 or gadD2D3 of the GAD system displayed reduced acid tolerance and were significantly affected in their ability to cause infection following oral inoculation. Since EGDm exploits GADi but not GADe the results indicate that the GADi system makes a contribution to virulence within the mouse. Furthermore, we also provide evidence that there might be a separate line of evolution in the GAD system between two commonly used reference strains
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Characterization of a Listeria monocytogenes Scott A Isolate with High Tolerance towards High Hydrostatic Pressure
An isolate of L. monocytogenes Scott A that is tolerant to high hydrostatic pressure (HHP), named AK01, was isolated upon a single pressurization treatment of 400 MPa for 20 min and was further characterized. The survival of exponential- and stationary-phase cells of AK01 in ACES [N-(2-acetamido)-2-aminoethanesulfonic acid] buffer was at least 2 log units higher than that of the wild type over a broad range of pressures (150 to 500 MPa), while both strains showed higher HHP tolerance (piezotolerance) in the stationary than in the exponential phase of growth. In semiskim milk, exponential-phase cells of both strains showed lower reductions upon pressurization than in buffer, but again, AK01 was more piezotolerant than the wild type. The piezotolerance of AK01 was retained for at least 40 generations in rich medium, suggesting a stable phenotype. Interestingly, cells of AK01 lacked flagella, were elongated, and showed slightly lower maximum specific growth rates than the wild type at 8, 22, and 30°C. Moreover, the piezotolerant strain AK01 showed increased resistance to heat, acid, and H(2)O(2) compared with the wild type. The difference in HHP tolerance between the piezotolerant strain and the wild-type strain could not be attributed to differences in membrane fluidity, since strain AK01 and the wild type had identical in situ lipid melting curves as determined by Fourier transform infrared spectroscopy. The demonstrated occurrence of a piezotolerant isolate of L. monocytogenes underscores the need to further investigate the mechanisms underlying HHP resistance of food-borne microorganisms, which in turn will contribute to the appropriate design of safe, accurate, and feasible HHP treatments
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Contingency locus in ctsR of Listeria monocytogenes Scott A: a strategy for occurrence of abundant piezotolerant isolates within clonal populations
In a recent study we demonstrated that a high-hydrostatic-pressure-tolerant isolate of Listeria monocytogenes
lacks a codon in the class 3 heat shock regulator gene ctsR. This mutation in the region that encodes four
consecutive glycines was directly responsible for the observed piezotolerance, increased stress resistance, and
reduced virulence. The aim of the present study was to determine whether mutations in ctsR are frequently
associated with piezotolerance in L. monocytogenes. Wild-type cultures of L. monocytogenes were therefore
exposed to 350 MPa for 20 min, and the piezotolerance of individual surviving isolates was assessed. This
rendered 33 isolates with a stable piezotolerant phenotype from a total of 84 survivors. Stable piezotolerant
mutants were estimated to be present in the initial wild-type population at frequencies of >10�5. Subsequent
sequencing of the ctsR gene of all stable piezotolerant isolates revealed that two-thirds of the strains (i.e., n �
21) had mutations in this gene. The majority of the mutations (16 of 21 strains) consisted of a triplet deletion
in the glycine-encoding region of ctsR, identical to what was found in our previous study. Interestingly, 2 of 21
mutants contained a codon insertion in this repeat region. The remaining three stable piezotolerant strains
showed a 19-bp insertion in the glycine repeat region, a 16-bp insertion downstream of the glycine repeat area
(both leading to frameshifts and a truncated ctsR), and an in-frame 114-bp deletion encoding a drastically
shortened carboxy terminus of CtsR. In four instances it was not possible to generate a PCR product. A
piezotolerant phenotype could not be linked to mutations in ctsR in 8 of 33 isolates, indicating that other
thus-far-unknown mechanisms also lead to stable piezotolerance. The present study highlights the importance
of ctsR in piezotolerance and stress tolerance of L. monocytogenes, and it demonstrates that short-sequence
repeat regions contribute significantly to the occurrence of a piezotolerant and stress-tolerant subpopulation
within L. monocytogenes cultures, thus playing an important role in survival
Loss of sigb in listeria monocytogenes strains egd-e and 10403s confers hyperresistance to hydrogen peroxide in stationary phase under aerobic conditions
SigB is the main stress gene regulator in Listeria monocytogenes affecting the expression of more than 150 genes and thus contributing to multiple-stress resistance. Despite its clear role in most stresses, its role in oxidative stress is uncertain, as results accompanying the loss of sigB range from hyperresistance to hypersensitivity. Previously, these differences have been attributed to strain variation. In this study, we show conclusively that unlike for all other stresses, loss of sigB results in hyperresistance to H2O2 (more than 8 log CFU ml(-1) compared to the wild type) in aerobically grown stationary-phase cultures of L. monocytogenes strains 10403S and EGD-e. Furthermore, growth at 30 degrees C resulted in higher resistance to oxidative stress than that at 37 degrees C. Oxidative stress resistance seemed to be higher with higher levels of oxygen. Under anaerobic conditions, the loss of SigB in 10403S did not affect survival against H2O2, while in EGD-e, it resulted in a sensitive phenotype. During exponential phase, minor differences occurred, and this result was expected due to the absence of sigB transcription. Catalase tests were performed under all conditions, and stronger catalase results corresponded well with a higher survival rate, underpinning the important role of catalase in this phenotype. Furthermore, we assessed the catalase activity in protein lysates, which corresponded with the catalase tests and survival. In addition, reverse transcription-PCR (RT-PCR) showed no differences in transcription between the wild type and the Delta sigB mutant in various oxidative stress genes. Further investigation of the molecular mechanism behind this phenotype and its possible consequences for the overall phenotype of L. monocytogenes are under way.
IMPORTANCE
SigB is the most important stress gene regulator in L. monocytogenes and other Gram-positive bacteria. Its increased expression during stationary phase results in resistance to multiple stresses. However, despite its important role in general stress resistance, its expression is detrimental for the cell in the presence of oxidative stress, as it promotes hypersensitivity against hydrogen peroxide. This peculiar phenotype is an important element of the physiology of L. monocytogenes, and it might help us explain the behavior of this organism in environments where oxidative stress is present
Phenotypic and Proteomic Characterization of Multiply Antibiotic-Resistant Variants of Salmonella enterica Serovar Typhimurium Selected Following Exposure to Disinfectantsâ–¿ â€
In previous work, Salmonella enterica serovar Typhimurium strain SL1344 was exposed to sublethal concentrations of three widely used farm disinfectants in daily serial passages for 7 days in an attempt to investigate possible links between the use of disinfectants and antimicrobial resistance. Stable variants OXCR1, QACFGR2, and TOPR2 were obtained following treatment with an oxidizing compound blend, a quaternary ammonium disinfectant containing formaldehyde and glutaraldehyde, and a tar acid-based disinfectant, respectively. All variants exhibited ca. fourfold-reduced susceptibility to ciprofloxacin, chloramphenicol, tetracycline, and ampicillin. This coincided with reduced levels of outer membrane proteins for all strains and high levels of AcrAB-TolC for OXCR1 and QACFGR2, as demonstrated by two-dimensional high-performance liquid chromatography-mass spectrometry. The protein profiles of OXCR1 and QACFGR2 were similar, but they were different from that of TOPR2. An array of different proteins protecting against oxidants, nitroaromatics, disulfides, and peroxides were overexpressed in all strains. The growth and motility of variants were reduced compared to the growth and motility of the parent strain, the expression of several virulence proteins was altered, and the invasiveness in an enteric epithelial cell line was reduced. The colony morphology of OXCR1 and QACFGR2 was smooth, and both variants exhibited a loss of modal distribution of the lipopolysaccharide O-antigen chain length, favoring the production of short O-antigen chain molecules. Metabolic changes were also detected, suggesting that there was increased protein synthesis and a shift from oxidative phosphorylation to substrate level phosphorylation. In this study, we obtained evidence that farm disinfectants can select for strains with reduced susceptibility to antibiotics, and here we describe changes in protein expression in such strains