Billions in Misspent EU Agricultural Subsidies Could Support the Sustainable Development Goals

The Common Agricultural Policy (CAP) is the guiding policy for agriculture and the largest single budget item in the European Union (EU). Agriculture is essential to meet the Sustainable Development Goals (SDGs), but the CAP's contribution to do so is uncertain. We analyzed the distribution of (sic)59.4 billion of 2015 CAP payments and show that current CAP spending exacerbates income inequality within agriculture, while little funding supports climate-friendly and biodiverse farming regions. More than (sic)24 billion of 2015 CAP direct payments went to regions where average farm incomes are already above the EU median income. A further (sic)2.5 billion in rural development payments went to primarily urban areas. Effective monitoring indicators are also missing. We recommend redirecting and better monitoring CAP payments toward achieving the environmental, sustainability, and rural development goals stated in the CAP's new objectives, which would support the SDGs, the European Green Deal, and green COVID-19 recovery

Book Reviews

Reviews of the following books: The Massachusetts Historical Society: A Bicentennial History, 1791-1991 by Louis Leonard Tucker; Interpreting Early American History Essays by Jack P. Greene; Molly Spotted Elk: A Penobscot in Paris by Bunny McBride; The Artist\u27s Mount Desert: American Painters on the Maine Coast by John Wilmerding; Politics of Conscience: A Biography of Margaret Chase Smith by Patricia Ward Wallace; Maine, A Peopled Landscape: Salt Documentary Photography, 1978 to 1995 edited by Hugh French, with essays by C. Stewart Doty, James C. Curtis and R. Todd Hoffman; Always Rachel: The Letters of Rachel Carson and Dorothy Freeman, 1952-1964 by Martha Free.man; Sarah Orne Jewett: Her World and Her Work by Paula Blanchar

KLF9 and JNK3 Interact to Suppress Axon Regeneration in the Adult CNS

Neurons in the adult mammalian CNS decrease in intrinsic axon growth capacity during development in concert with changes in Krüppel-like transcription factors (KLFs). KLFs regulate axon growth in CNS neurons including retinal ganglion cells (RGCs). Here, we found that knock-down of KLF9, an axon growth suppressor that is normally upregulated 250-fold in RGC development, promotes long-distance optic nerve regeneration in adult rats of both sexes. We identified a novel binding partner, MAPK10/JNK3 kinase, and found that JNK3 (c-Jun N-terminal kinase 3) is critical for KLF9\u27s axon-growth-suppressive activity. Interfering with a JNK3-binding domain or mutating two newly discovered serine phosphorylation acceptor sites, Ser106 and Ser110, effectively abolished KLF9\u27s neurite growth suppression in vitro and promoted axon regeneration in vivo. These findings demonstrate a novel, physiologic role for the interaction of KLF9 and JNK3 in regenerative failure in the optic nerve and suggest new therapeutic strategies to promote axon regeneration in the adult CNS

Methyl-CpG-binding domain sequencing reveals a prognostic methylation signature in neuroblastoma

Accurate assessment of neuroblastoma outcome prediction remains challenging. Therefore, this study aims at establishing novel prognostic tumor DNA methylation biomarkers. In total, 396 low- and high-risk primary tumors were analyzed, of which 87 were profiled using methyl-CpG-binding domain (MBD) sequencing for differential methylation analysis between prognostic patient groups. Subsequently, methylation-specific PCR (MSP) assays were developed for 78 top-ranking differentially methylated regions and tested on two independent cohorts of 132 and 177 samples, respectively. Further, a new statistical framework was used to identify a robust set of MSP assays of which the methylation score (i.e. the percentage of methylated assays) allows accurate outcome prediction. Survival analyses were performed on the individual target level, as well as on the combined multimarker signature. As a result of the differential DNA methylation assessment by MBD sequencing, 58 of the 78 MSP assays were designed in regions previously unexplored in neuroblastoma, and 36 are located in non-promoter or non-coding regions. In total, 5 individual MSP assays (located in CCDC177, NXPH1, lnc-MRPL3-2, lnc-TREX1-1 and one on a region from chromosome 8 with no further annotation) predict event-free survival and 4 additional assays (located in SPRED3, TNFAIP2, NPM2 and CYYR1) also predict overall survival. Furthermore, a robust 58-marker methylation signature predicting overall and event-free survival was established. In conclusion, this study encompasses the largest DNA methylation biomarker study in neuroblastoma so far. We identified and independently validated several novel prognostic biomarkers, as well as a prognostic 58-marker methylation signature

Dengue Reporter Virus Particles for Measuring Neutralizing Antibodies against Each of the Four Dengue Serotypes

The lack of reliable, high-throughput tools for characterizing anti-dengue virus (DENV) antibodies in large numbers of serum samples has been an obstacle in understanding the impact of neutralizing antibodies on disease progression and vaccine efficacy. A reporter system using pseudoinfectious DENV reporter virus particles (RVPs) was previously developed by others to facilitate the genetic manipulation and biological characterization of DENV virions. In the current study, we demonstrate the diagnostic utility of DENV RVPs for measuring neutralizing antibodies in human serum samples against all four DENV serotypes, with attention to the suitability of DENV RVPs for large-scale, long-term studies. DENV RVPs used against human sera yielded serotype-specific responses and reproducible neutralization titers that were in statistical agreement with Plaque Reduction Neutralization Test (PRNT) results. DENV RVPs were also used to measure neutralization titers against the four DENV serotypes in a panel of human sera from a clinical study of dengue patients. The high-throughput capability, stability, rapidity, and reproducibility of assays using DENV RVPs offer advantages for detecting immune responses that can be applied to large-scale clinical studies of DENV infection and vaccination

Baylisascaris procyonis in the Metropolitan Atlanta Area

Baylisascaris procyonis, the raccoon roundworm responsible for fatal larva migrans in humans, has long been thought to be absent from many regions in the southeastern United States. During spring 2002, 11 (22%) of 50 raccoons trapped in DeKalb County, Georgia, had B. procyonis infection. The increasing number of cases highlight this emerging zoonotic infection

Religiosity, Spirituality, and the Subjective Quality of African American Men's Friendships: An Exploratory Study

The present study fills a crucial gap in literature surrounding the lives of African American men by exploring factors that shape the quality of these men's friendships. Drawing on data from a sample of 171 African American men, the study examines the relative utility of subjective religiosity, subjective spirituality, advice exchange, and affective sharing as predictors of the level of perceived support from male and female friends. Findings reveal age differences in subjective religiosity, subjective spirituality, and in level of advice and affective exchange in men's same-sex as well as cross-sex friendships. Age differences emerged in men's perceptions of the supportiveness of their friendships with women but not with men. Age was not a predictor of perceived supportiveness of same-sex or cross-sex friendships. Subjective religiosity did not predict support in same-sex or cross-sex friendships. Subjective spirituality positively predicted perceived support in men's same-sex friendships but not in cross-sex friendships. Implications of these findings are discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44637/1/10804_2004_Article_341947.pd

A New Sample of Transient Ultraluminous X-Ray Sources Serendipitously Discovered by Swift/XRT

© 2023. The Author(s). Published by the American Astronomical Society. This is an open access article distributed under the Creative Commons Attribution License, to view a copy of the license, see: https://creativecommons.org/licenses/by/4.0/Ultraluminous X-ray sources (ULXs) are our best laboratories for studying extreme super-Eddington accretion. Most studies of these objects are of relatively persistent sources; however, there is growing evidence to suggest a large fraction of these sources are transient. Here we present a sample of five newly reported transient ULXs in the galaxies NGC 4945, NGC 7793, and M81 serendipitously discovered in Swift/XRT observations. Swift monitoring of these sources have provided well-sampled lightcurves, allowing for us to model the lightcurves with the disk-instability model of Hameury & Lasota, which implies durations of 60–400 days and that the mass-accretion rate through the disk is close to or greater than the Eddington rate. Of the three source regions with prior Hubble Space Telescope imaging, color–magnitude diagrams of the potential stellar counterparts show varying ages of the possible stellar counterparts. Our estimation of the rates of these sources in these three galaxies is 0.4–1.3 yr−1. We find that, while persistent ULXs dominate the high end of galaxy luminosity functions, the number of systems that produce ULX luminosities are likely dominated by transient sources.Peer reviewe

Understanding Evolutionary Impacts of Seasonality: An Introduction to the Symposium

Seasonality is a critically important aspect of environmental variability, and strongly shapes all aspects of life for organisms living in highly seasonal environments. Seasonality has played a key role in generating biodiversity, and has driven the evolution of extreme physiological adaptations and behaviors such as migration and hibernation. Fluctuating selection pressures on survival and fecundity between summer and winter provide a complex selective landscape, which can be met by a combination of three outcomes of adaptive evolution: genetic polymorphism, phenotypic plasticity, and bet-hedging. Here, we have identified four important research questions with the goal of advancing our understanding of evolutionary impacts of seasonality. First, we ask how characteristics of environments and species will determine which adaptive response occurs. Relevant characteristics include costs and limits of plasticity, predictability, and reliability of cues, and grain of environmental variation relative to generation time. A second important question is how phenological shifts will amplify or ameliorate selection on physiological hardiness. Shifts in phenology can preserve the thermal niche despite shifts in climate, but may fail to completely conserve the niche or may even expose life stages to conditions that cause mortality. Considering distinct environmental sensitivities of life history stages will be key to refining models that forecast susceptibility to climate change. Third, we must identify critical physiological phenotypes that underlie seasonal adaptation and work toward understanding the genetic architectures of these responses. These architectures are key for predicting evolutionary responses. Pleiotropic genes that regulate multiple responses to changing seasons may facilitate coordination among functionally related traits, or conversely may constrain the expression of optimal phenotypes. Finally, we must advance our understanding of how changes in seasonal fluctuations are impacting ecological interaction networks. We should move beyond simple dyadic interactions, such as predator prey dynamics, and understand how these interactions scale up to affect ecological interaction networks. As global climate change alters many aspects of seasonal variability, including extreme events and changes in mean conditions, organisms must respond appropriately or go extinct. The outcome of adaptation to seasonality will determine responses to climate change