ATUM-FIB microscopy for targeting and multiscale imaging of rare events in mouse cortex

Here, we describe a detailed workflow for ATUM-FIB microscopy, a hybrid method that combines serial-sectioning scanning electron microscopy (SEM) with focused ion beam SEM (FIB-SEM). This detailed protocol is optimized for mouse cortex samples. The main processing steps include the generation of semi-thick sections from sequentially cured resin blocks using a heated microtomy approach. We demonstrate the different imaging modalities, including serial light and electron microscopy for target recognition and FIB-SEM for isotropic imaging of regions of interest.For complete details on the use and execution of this protocol, please refer to Kislinger et al. (2020)

Die Resultate der zweiten Gepatschfernervermessung

von Georg Kerschensteine