Effects of prepartum treatment with monensin or propylene glycol mixed with concentrate on milk yield and blood NEFA and BHBA levels in dairy cows

Sixty multiparous Holstein-Friesian dairy cows made up the study sample. Group M (n = 20) received 300 mg/day monensin (Kexxtone, Elanco) for 21 days prior to the expected calving date. Group PGC (n = 20) received propylene glycol, mixed with concentrate and fed separately from total mixed ration (TMR) twice a day in the last 21 days of gestation. The control group, Group C(n = 20) did not receive any treatment prior to parturition. Cows in all three groups received PG (300 mL/day) for 5 days after parturition. Blood samples were collected 21 and 7 days prior to the expected calving date, as well as 7 and 14 days after parturition. NEFA and BHBA levels did not differ between the treatment groups. However, milk yield of Group M was significantly higher than that of Group C during weeks 3, 4, and 5 of lactation. Although NEFA and BHBA levels were similar in both treatment groups, higher milk yield in monensin-treated cows could be related to an increase in glycogenic precursors resulting from favorable effects of monensin on ruminal bacterial flora

Serological diagnosis of toxoplasmosis and determination of seroprevalence of anti-Toxoplasma gondii antibodies in cattle in Bursa province

Bu çalışmayla Bursa bölgesindeki sığırlarda toxoplasmosisin serolojik tanısı ve anti-Toxoplasma gondii antikor seroprevalansının belirlenmesi amaçlandı. Çalışmanın materyalini Bursa ve çevresindeki altı farklı ilçeden rastgele seçilen 2 ay-5 yaşlı, Holstein ırkı 200 adet sağlıklı sığır oluşturdu. Tüm hayvanların klinik muayeneleri yapıldı ve kan örnekleri toplandı.An-T.T.Toxsoplasma gondii antikorları Sabin Feldman tekniği ile analiz edildi. Çalışmada 200 sığırın 146'smda (%73) anti-T. gondii antikorları saptanırken 54 (%27) sığırın seronegatif olduğu belirlendi. En yüksek seropozitiflik oranı Keleş (%86.2) en düşük oran ise Merkez ilçede (%63) belirlendi. Anti -T. gondii antikor seroprevalansının 0-1 yaş grubunda diğer yaş gruplarından daha yüksek oranda (%80.3) olduğu saptandı. Seropozitif ve seronegatif sığırların hematolojik muayenelerinde bir farklılık saptanmadı. Sonuç olarak, bu çalışmada Bursa bölgesindeki sığırlarda toxoplasmosis seroprevalansının yüksek düzeyde olduğu ve hastalığa gençlerin daha duyarlı oldukları saptandı. Halk sağlığı açısından önemli bir enfeksiyon olan toxoplasmosise karşı profilaktif önlemlerin alınmasının gerekli olduğu kanısına varıldı.Serological diagnosis of toxoplasmosis and determination of seroprevalence of anti- Toxoplasma gondii antibodies in cattle in Bursa province were aimed in this study. The material of the study was consisted of randomly selected twohundred healthy Holstein cattle aged 2 month - 5 year from six different districts in Bursa province. Cli­nical examinations were performed and blood samples were collected from all cattle. Anti-Toxoplasma gondii an­tibodies were analyzed with Sabin Feldman test. Anti - T. gondii antibodies were detected in 146 (73%) of 200 cattle and 54 (27%) cattle were found seronegative. Higher seropositivity was observed in Keles (86.2%) whereas lower se-ropositivity was found in Central district (63%). No difference was detected in hematological analysis of seropositive and seronegative cattle. As a result, it was determined that seroprevalance of toxoplasmosis was high in cattle in Bursa region and it was found that young cattle were more susceptible to ihfection in this study. It was concluded that prophylactic procedures must be performed in cattle against toxoplasmosis which is an important zoonotic infection for public health

A pilot study on the epidemiological status of equine infectious anaemia, equine viral arteritis, glanders, and dourine in Turkey

A serological investigation of equine infectious anaemia (EIA), equine viral arteritis (EAV), glanders, and dourine was conducted on the sera of 346 Turkish horses using a combination of tests in series (ELISA and agar gel immunodiffusion) for EIA, virus neutralisation for EAV, and complement fixation (CF) for glanders and dourine. Forty-nine sera showed anticomplementary reactions and were not assessable in the CF test for glanders and dourine. No positive samples were detected for EIA, dourine, and glanders. Fifty-seven sera were positive for EAV. A systematic review of the distribution of these diseases in Turkey was conducted to describe their epidemiological status in the country. The serological results of this investigation confirm those of the published reports for EAV and glanders, whereas different results were reported for dourine and EIA. In fact, no previous data were found for dourine. Furthermore, all sera tested for EIA in the literature were negative, but 3 outbreaks were reported on an international official site in 2005 without details. Further studies and reports of the outbreaks are needed to better understand the real status of infections and the transmission of the diseases. The systematic review is a useful tool to improve the knowledge of public health disease in a country