Nitric oxide-cGMP signaling regulates axonal elongation during optic nerve regeneration in the goldfish in vitro and in vivo

金沢大学医薬保健研究域　医学系Nitric oxide (NO) signaling results in both neurotoxic and neuroprotective effects in CNS and PNS neurons, respectively, after nerve lesioning. We investigated the role of NO signaling on optic nerve regeneration in the goldfish (Carassius auratus). NADPH diaphorase staining revealed that nitric oxide synthase (NOS) activity was up-regulated primarily in the retinal ganglion cells (RGCs) 5-40 days after axotomy. Levels of neuronal NOS (nNOS) mRNA and protein also increased in the RGCs alone during this period. This period (5-40 days) overlapped with the process of axonal elongation during regeneration of the goldfish optic nerve. Therefore, we evaluated the effect of NO signaling molecules upon neurite outgrowth from adult goldfish axotomized RGCs in culture. NO donors and dibutyryl cGMP increased neurite outgrowth dose-dependently. In contrast, a nNOS inhibitor and small interfering RNA, specific for the nNOS gene, suppressed neurite outgrowth from the injured RGCs. Intra-ocular dibutyryl cGMP promoted the axonal regeneration from injured RGCs in vivo. None of these molecules had an effect on cell death/survival in this culture system. This is the first report showing that NO-cGMP signaling pathway through nNOS activation is involved in neuroregeneration in fish CNS neurons after nerve lesioning. © 2009 International Society for Neurochemistry.出版社許諾要件により、2010年9月より全文公開

SDSS J1029+2623: A Gravitationally Lensed Quasar with an Image Separation of 22.5 Arcseconds

We report the discovery of a cluster-scale lensed quasar, SDSS J1029+2623, selected from the Sloan Digital Sky Survey. The lens system exhibits two lensed images of a quasar at z_s=2.197. The image separation of 22.5" makes it the largest separation lensed quasar discovered to date. The similarity of the optical spectra and the radio loudnesses of the two components support the lensing hypothesis. Images of the field show a cluster of galaxies at z_l~0.55 that is responsible for the large image separation. The lensed images and the cluster light center are not collinear, which implies that the lensing cluster has a complex structure.Comment: 5 pages, 4 figures. Accepted for publication in ApJ Letter

Real-world evidence of the impact of obesity on residual teeth in the Japanese population : A cross-sectional study

Background:Tooth loss is associated with nutritional status and significantly affects quality of life, particularly in older individuals. To date, several studies reveal that a high BMI is associated with tooth loss. However, there is a lack of large-scale studies that examined the impact of obesity on residual teeth with respect to age and tooth positions.Objective:We assessed the impact of obesity on the number and position of residual teeth by age groups using large scale of Japanese database.Methods:This was a cross-sectional study of 706150 subjects that were included in the database that combined the data from health insurance claims and health check-up, those lacking information about BMI, HbA1c level, smoking status, and the number of residual teeth were excluded. Thus, a total of 233517 aged 20-74 years were included. Subjects were classified into 4 categories based on BMI, and the number of teeth was compared between age-groups. The percentage of subjects with residual teeth in each position was compared between groups with obesity (BMI ≥25.0 kg/m2) and non-obesity. Logistic regression analysis was performed to clarify whether obesity predicts having <24 teeth.Results:Higher BMI was associated with fewer teeth over 40s (P for trend <0.0001 when <70s). Obesity was associated with the reduction of residual teeth in the maxillary; specifically, the molars were affected over the age 30. Smoking status further affected tooth loss at positions that were not affected by obesity alone. After adjusting for age, sex, smoking status, and HbA1c ≥6.5%, obesity remained an independent predictive factor for having <24 teeth (ORs: 1.35, 95% CIs: 1.30-1.40).Conclusions:We found that an increase in BMI was associated with a decrease in the number of residual teeth from younger ages independently of smoking status and diabetes in the large scale of Japanese database

Dual supermassive black holes at close separation revealed by the Hyper Suprime-Cam Subaru Strategic Program

The unique combination of superb spatial resolution, wide-area coverage, and deep depth of the optical imaging from the Hyper Suprime-Cam (HSC) Subaru Strategic Program is utilized to search for dual quasar candidates. Using an automated image analysis routine on 34,476 known SDSS quasars, we identify those with two (or more) distinct optical point sources in HSC images covering 796 deg^2. We find 421 candidates out to a redshift of 4.5 of which one hundred or so are more likely after filtering out contaminating stars. Angular separations of 0.6 - 4.0" correspond to projected separations of 3 - 30 kpc, a range relatively unexplored for population studies of luminous dual quasars. Using Keck-I/LRIS and Gemini-N/NIFS, we spectroscopically confirm three dual quasar systems at z < 1, two of which are previously unknown out of eight observed, based on the presence of characteristic broad emission lines in each component, while highlighting that the continuum of one object in one of the pairs is reddened. In all cases, the [OIII]5007 emission lines have mild velocity offsets, thus the joint [OIII] line profile is not double-peaked. We find a dual quasar fraction of 0.26+/-0.18% and no evidence for evolution. A comparison with the Horizon-AGN simulation seems to support the case of no evolution in the dual quasar fraction when broadly matching the quasar selection. These results may indicate a scenario in which the frequency of the simultaneous triggering of luminous quasars is not as sensitive as expected to the cosmic evolution of the merger rate or gas content of galaxies.Comment: 11 pages; 12 figures; Accepted for publication in The Astrophysical Journa