Caracterización química y morfológica de Allium tuncelianum (Amaryllidaceae) y potenciales antioxidantes y anticolinesterasa

Alzheimer’s disease is the main reason for dementia, which increases with age. Cholinesterase inhibition and antioxidant potentials of extracts and essential oils from bulbs of A. tuncelianum (Kollmann) Özhatay & al., an endemic species to Tunceli (easthern Turkey), were evaluated. The fraction extracted of ethyl acetate had the highest phenolics level, 1,1-diphenyl-2-picrylhydrazyl, and thiobarbituric acid antioxidant capacity. Also, the ethyl acetate fraction presented the highest acetylcholinesterase (15.98 ± 2.76%), and butyrylcholinesterase inhibition (47.33 ± 3.27%). Diallyl disulfide (49.8%), diallyl trisulfide (27.9%) and allyl methyl trisulfide (6.9%) were found to be the major components of essential oil. This paper shows that the ethyl acetate fraction of A. tuncelianum could be a potent source of antioxidant and anticholinesterase components.La enfermedad de Alzheimer es la causa principal de la demencia, cuya aparición aumenta según la edad. Se evaluaron la inhibición de la colinesterasa y el potencial antioxidante de los extractos y los aceites esenciales de los bulbos de A. tuncelianum (Kollmann) Özhatay & al., una especie endémica de Tunceli (este de Turquía). La fracción extraida de acetato de etilo presentó los niveles más altos de fenoles, 1,1-difenil- 2-picrilhidrazilo y capacidad antioxidante, ácido tiobarbitúrico. Asimismo, la fracción de etil acetato presentó la mayor capacidad de inhibición de acetilcolinesterasa (15.98 ± 2.76%) y butirilcolinesterasa (47.33 ± 3.27%). El disulfuro de dialilo (49.8%), el trisulfuro de dialilo (27.9%) y el trisulfuro de metil alilo (6.9%) fueron los componentes principales del aceite esencial. Este artículo muestra que la fracción de etil acetato de A. tuncelianum podría ser una fuente potencial novedosa de componentes antioxidantes y anticolinesterasa

Antioxidant and Antiradical Activity of Beetroot (Beta vulgaris L. var. conditiva Alef.) Grown Using Different Fertilizers

Fertilizers in different nitrogen forms (calcium ammonium nitrate (CAN), urea, ammonium sulfate (AS), and ammonium nitrate (AN)) and their doses (50, 100, and 150) for beetroot (BT) (Beta vulgaris L. var. conditiva Alef.) and the antioxidant and antiradical activities in the lyophilized water and alcohol extracts of BT were evaluated. In order to evaluate antioxidant and radical removing activities of BT roots, total phenolic compound amount assignment, total flavonoids amount assignment, method of Fe3+-Fe2+ reduction activity using ferric cyanate reduction, cupric ions (Cu2+) reducing capacity with CUPRAC method, Fe3+ reducing capacity according to FRAP method, ferrous ions (Fe2+) chelating activity, superoxide anion radical (O2•-) removing activity, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) radical removing activity were determined. In the study, BHA and α-tocopherol were used as standard antioxidants. It was determined that water and alcohol extracts obtained from BT roots indicated reduction activities, effectively. In addition, it was also determined that these reduction activities were indicated in most BT roots grown in fertilizer media at lower percentage and that they had higher antioxidative level than that of standard antioxidants

Antibacterial Activities of Red Colored Radish Types (Raphanus sativus L.)

The root parts of red skin colored radish cultivars cv. Antep and cv. Cherry Belle which are belongs to Raphanus sativus L. were harvested from Erzurum region of Turkey and their methanol extracts were evaluated for antibacterial activity against 52 food borne bacteria by using disc diffusion assay. Extracts had a broad-spectrum antibacterial activity against food borne bacteria in broth micro dilution bioassays. Maximum antibacterial activity was observed against Arthrobacter ilicis, Corynebacterium ammoniagenes, Enterobacter hormaechei, Kocuria rosea, Neisseria subflava, Pantoea agglomerans, Proteus vulgaris, Psychrobacter immobilis and Shigella dysenteriae. However, cv. Antep showed antibacterial activity against Bacillus sphaericus and Corynebacterium flavescens and cv. Cherry Belle showed antibacterial activity against Arthrobacter atrocyaneus as well as. These inhibitory effects are interesting in relation to the prevention of microbial contamination in many foods

IN VITRO ANTIBACTERIAL ACTIVITIES OF BLACK AND WHITE RADISHES (RAPHANUS SATIVUS L.)

The present study was conducted to evaluate the in vitro antibacterial activities of methanol extracts of white and black coloured peel taproots of two radish types belonging to Raphanus sativus L. The antibacterial activities of the extracts were tested against 100 laboratory strains belonging to 52 bacteria species by using disc diffusion assay. The results showed that methanol extract in taproots of both black and white radishes had antibacterial activity against Arthrobacter atrocyaneus, Corynebacterium ammoniagenes, Enterobacter hormaechei, Kocuria rosea, Neisseria subflava, Pantoea agglomerans, Proteus vulgaris, Psychrobacter immobilis and Shigella dysenteriae. White radish type showed antibacterial activity against Bacillus sphaericus and Corynebacterium flavescens as well. Based on results, it is possible to conclude that white radish has a stronger and broader spectrum of antibacterial activity as compared to black radish

Chemical and morphological characterization of "Allium tuncelianum (Amaryllidaceae)" and its antioxidant and anticholinesterase potentials

Alzheimer’s disease is the main reason for dementia, which increases with age. Cholinesterase inhibition and antioxidant potentials of extracts and essential oils from bulbs of A. tuncelianum (Kollmann) Özhatay & al., an endemic species to Tunceli (easthern Turkey), were evaluated. The fraction extracted of ethyl acetate had the highest phenolics level, 1,1-diphenyl-2-picrylhydrazyl, and thiobarbituric acid antioxidant capacity. Also, the ethyl acetate fraction presented the highest acetylcholinesterase (15.98 ± 2.76%), and butyrylcholinesterase inhibition (47.33 ± 3.27%). Diallyl disulfide (49.8%), diallyl trisulfide (27.9%) and allyl methyl trisulfide (6.9%) were found to be the major components of essential oil. This paper shows that the ethyl acetate fraction of A. tuncelianum could be a potent source of antioxidant and anticholinesterase components.La enfermedad de Alzheimer es la causa principal de la demencia, cuya aparición aumenta según la edad. Se evaluaron la inhibición de la colinesterasa y el potencial antioxidante de los extractos y los aceites esenciales de los bulbos de A. tuncelianum (Kollmann) Özhatay & al., una especie endémica de Tunceli (este de Turquía). La fracción extraida de acetato de etilo presentó los niveles más altos de fenoles, 1,1-difenil2-picrilhidrazilo y capacidad antioxidante, ácido tiobarbitúrico. Asimismo, la fracción de etil acetato presentó la mayor capacidad de inhibición de acetilcolinesterasa (15.98 ± 2.76%) y butirilcolinesterasa (47.33 ± 3.27%). El disulfuro de dialilo (49.8%), el trisulfuro de dialilo (27.9%) y el trisulfuro de metil alilo (6.9%) fueron los componentes principales del aceite esencial. Este artículo muestra que la fracción de etil acetato de A. tuncelianum podría ser una fuente potencial novedosa de componentes antioxidantes y anticolinesterasa