Evaluation of the consistency of fiber reinforced cementitious composites

The rheological properties of fresh concrete, mortar or cement paste are among the most important parameters when cementitious building materials are placed. New material designs, like high or ultrahigh performance concretes, include the addition of a high volume of fibers to the fresh mix influencing its workability properties. However, the analysis of the rheological properties of fiber reinforced cementitious composites is difficult. Conventional methods mostly do not apply, especially when a high fiber content and relatively stiff mixtures are used. For this reason, a new method was developed to evaluate the workability of fiber reinforced composites. This method was applied to carbon and PVA fiber reinforced high performance composites and was used to optimize the rheological properties of these composites for an application in a centrifugation casting proces

Rectilinear Planarity of Partial 2-Trees

A graph is rectilinear planar if it admits a planar orthogonal drawing without bends. While testing rectilinear planarity is NP-hard in general (Garg and Tamassia, 2001), it is a long-standing open problem to establish a tight upper bound on its complexity for partial 2-trees, i.e., graphs whose biconnected components are series-parallel. We describe a new O(n^2)-time algorithm to test rectilinear planarity of partial 2-trees, which improves over the current best bound of O(n^3 \log n) (Di Giacomo et al., 2022). Moreover, for partial 2-trees where no two parallel-components in a biconnected component share a pole, we are able to achieve optimal O(n)-time complexity. Our algorithms are based on an extensive study and a deeper understanding of the notion of orthogonal spirality, introduced several years ago (Di Battista et al, 1998) to describe how much an orthogonal drawing of a subgraph is rolled-up in an orthogonal drawing of the graph.Comment: arXiv admin note: substantial text overlap with arXiv:2110.00548 Appears in the Proceedings of the 30th International Symposium on Graph Drawing and Network Visualization (GD 2022

Artificial intelligence - finite element method - hybrids for efficient nonlinear analysis of concrete structures

Realistic structural analyses and optimisations using the non-linear finite element method are possible today yet suffer from being very time-consuming, particularly in case of reinforced concrete plates and shells. Hence such investigations are currently dismissed in the vast majority of cases in practice. The "Artificial Intelligence - Finite Element - Hybrids" project addresses the current unsatisfactory situation with an approach that combines non-linear finite element models for reinforced concrete shells with scientific machine learning algorithms to create hybrid AI-FEM models. The AI-based surrogate material model provides the material stiffness as well as the stress tensor for given concrete design parameters and the strain tensor. This paper reports on the current status of the project and findings of the calibration of the AI-based reinforced concrete material model. We successfully calibrated and evaluated k-nearest-neighbour, LGBM and ResNet algorithms and report their predictive capabilities. Finally, some light is shed on the future work of integrating the AI surrogate material models back into the finite element method in the course of the numerical analysis of reinforced concrete structures

Toll-like receptor 4 deficiency facilitates α-synuclein propagation and neurodegeneration in a mouse model of prodromal Parkinson's disease

The evidence linking innate immunity mechanisms and neurodegenerative diseases is growing, but the specific mechanisms are incompletely understood. Experimental data suggest that microglial TLR4 mediates the uptake and clearance of α-synuclein also termed synucleinophagy. The accumulation of misfolded α-synuclein throughout the brain is central to Parkinson's disease (PD). The distribution and progression of the pathology is often attributed to the propagation of α-synuclein. Here, we apply a classical α-synuclein propagation model of prodromal PD in wild type and TLR4 deficient mice to study the role of TLR4 in the progression of the disease. Our data suggest that TLR4 deficiency facilitates the α-synuclein seed spreading associated with reduced lysosomal activity of microglia. Three months after seed inoculation, more pronounced proteinase K-resistant α-synuclein inclusion pathology is observed in mice with TLR4 deficiency. The facilitated propagation of α-synuclein is associated with early loss of dopamine transporter (DAT) signal in the striatum and loss of dopaminergic neurons in substantia nigra pars compacta of TLR4 deficient mice. These new results support TLR4 signaling as a putative target for disease modification to slow the progression of PD and related disorders

Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis

Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated in many aspects of plant growth, development, intra- and inter-cellular signaling, nutrient uptake and pathogen defense. Despite these significant roles, little is known about the precise molecular details of how it functions in planta. In order to facilitate the direct quantitative study of plant CME, here we review current routinely used methods and present refined, standardized quantitative imaging protocols which allow the detailed characterization of CME at multiple scales in plant tissues. These include: (i) an efficient electron microscopy protocol for the imaging of Arabidopsis CME vesicles in situ, thus providing a method for the detailed characterization of the ultra-structure of clathrin-coated vesicles; (ii) a detailed protocol and analysis for quantitative live-cell fluorescence microscopy to precisely examine the temporal interplay of endocytosis components during single CME events; (iii) a semi-automated analysis to allow the quantitative characterization of global internalization of cargos in whole plant tissues; and (iv) an overview and validation of useful genetic and pharmacological tools to interrogate the molecular mechanisms and function of CME in intact plant samples

Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants

In plants, clathrin mediated endocytosis (CME) represents the major route for cargo internalisation from the cell surface. It has been assumed to operate in an evolutionary conserved manner as in yeast and animals. Here we report characterisation of ultrastructure, dynamics and mechanisms of plant CME as allowed by our advancement in electron microscopy and quantitative live imaging techniques. Arabidopsis CME appears to follow the constant curvature model and the bona fide CME population generates vesicles of a predominantly hexagonal-basket type; larger and with faster kinetics than in other models. Contrary to the existing paradigm, actin is dispensable for CME events at the plasma membrane but plays a unique role in collecting endocytic vesicles, sorting of internalised cargos and directional endosome movement that itself actively promote CME events. Internalized vesicles display a strongly delayed and sequential uncoating. These unique features highlight the independent evolution of the plant CME mechanism during the autonomous rise of multicellularity in eukaryotes

Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses

Many central synapses contain a single presynaptic active zone and a single postsynaptic density. Vesicular release statistics at such “simple synapses” indicate that they contain a small complement of docking sites where vesicles repetitively dock and fuse. In this work, we investigate functional and morphological aspects of docking sites at simple synapses made between cerebellar parallel fibers and molecular layer interneurons. Using immunogold labeling of SDS-treated freeze-fracture replicas, we find that Cav2.1 channels form several clusters per active zone with about nine channels per cluster. The mean value and range of intersynaptic variation are similar for Cav2.1 cluster numbers and for functional estimates of docking-site numbers obtained from the maximum numbers of released vesicles per action potential. Both numbers grow in relation with synaptic size and decrease by a similar extent with age between 2 wk and 4 wk postnatal. Thus, the mean docking-site numbers were 3.15 at 2 wk (range: 1–10) and 2.03 at 4 wk (range: 1–4), whereas the mean numbers of Cav2.1 clusters were 2.84 at 2 wk (range: 1–8) and 2.37 at 4 wk (range: 1–5). These changes were accompanied by decreases of miniature current amplitude (from 93 pA to 56 pA), active-zone surface area (from 0.0427 μm2 to 0.0234 μm2), and initial success rate (from 0.609 to 0.353), indicating a tightening of synaptic transmission with development. Altogether, these results suggest a close correspondence between the number of functionally defined vesicular docking sites and that of clusters of voltage-gated calcium channels

Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants

In plants, clathrin mediated endocytosis (CME) represents the major route for cargo internalisation from the cell surface. It has been assumed to operate in an evolutionary conserved manner as in yeast and animals. Here we report characterisation of ultrastructure, dynamics and mechanisms of plant CME as allowed by our advancement in electron microscopy and quantitative live imaging techniques. Arabidopsis CME appears to follow the constant curvature model and the bona fide CME population generates vesicles of a predominantly hexagonal-basket type; larger and with faster kinetics than in other models. Contrary to the existing paradigm, actin is dispensable for CME events at the plasma membrane but plays a unique role in collecting endocytic vesicles, sorting of internalised cargos and directional endosome movement that itself actively promote CME events. Internalized vesicles display a strongly delayed and sequential uncoating. These unique features highlight the independent evolution of the plant CME mechanism during the autonomous rise of multicellularity in eukaryotes