Appendix A. A community-disassembly table for the 13 species of mammals and birds at our field site in Dutchess County, New York, USA, giving parameters used in models relating vertebrate community composition to nymphal tick infection prevalence (NIP).

A community-disassembly table for the 13 species of mammals and birds at our field site in Dutchess County, New York, USA, giving parameters used in models relating vertebrate community composition to nymphal tick infection prevalence (NIP)

Prevalence of human-active and variant 1 strains of the tick-borne pathogen Anaplasma phagocytophilum in hosts and forests of eastern North America

Anaplasmosis is an emerging infectious disease caused by infection with the bacterium Anaplasma phagocytophilum. In the eastern United States, A. phagocytophilum is transmitted to hosts through the bite of the blacklegged tick, Ixodes scapularis. We determined the realized reservoir competence of 14 species of common vertebrate hosts for ticks by establishing the probability that each species transmits two important strains of A. phagocytophilum (A. phagocytophilum human-active, which causes human cases, and A. phagocytophilum variant 1, which does not) to feeding larval ticks. We also sampled questing nymphal ticks from ∼ 150 sites in a single county over 2 years and sampled over 6 years at one location. White-footed mice (Peromyscus leucopus) and Eastern chipmunks (Tamias striatus) were the most competent reservoirs for infection with the A. phagocytophilum human-active strain. Across the county, prevalence in ticks for both strains together was 8.3%; ticks were more than two times as likely to be infected with A. phagocytophilum human-active as A. phagocytophilum variant 1

Co-infection of blacklegged ticks with Babesia microti and Borrelia burgdorferi is higher than expected and acquired from small mammal hosts

Humans in the northeastern and midwestern United States are at increasing risk of acquiring tickborne diseases--not only Lyme disease, but also two emerging diseases, human granulocytic anaplasmosis and human babesiosis. Co-infection with two or more of these pathogens can increase the severity of health impacts. The risk of co-infection is intensified by the ecology of these three diseases because all three pathogens (Borrelia burgdorferi, Anaplasma phagocytophilum, and Babesia microti) are transmitted by the same vector, blacklegged ticks (Ixodes scapularis), and are carried by many of the same reservoir hosts. The risk of exposure to multiple pathogens from a single tick bite and the sources of co-infected ticks are not well understood. In this study, we quantify the risk of co-infection by measuring infection prevalence in 4,368 questing nymphs throughout an endemic region for all three diseases (Dutchess County, NY) to determine if co-infections occur at frequencies other than predicted by independent assortment of pathogens. Further, we identify sources of co-infection by quantifying rates of co-infection on 3,275 larval ticks fed on known hosts. We find significant deviations of levels of co-infection in questing nymphs, most notably 83% more co-infection with Babesia microti and Borrelia burgdorferi than predicted by chance alone. Further, this pattern of increased co-infection was observed in larval ticks that fed on small mammal hosts, but not on meso-mammal, sciurid, or avian hosts. Co-infections involving A. phagocytophilum were less common, and fewer co-infections of A. phagocytophilum and B. microti than predicted by chance were observed in both questing nymphs and larvae fed on small mammals. Medical practitioners should be aware of the elevated risk of B. microti/B. burgdorferi co-infection

Transforming ecological science at primarily undergraduate institutions through collaborative networks

Ecologists at primarily undergraduate institutions (PUIs) are well positioned to form collaborative networks and make transformative contributions to the study and teaching of ecology. The spatial and temporal complexity of ecological phenomena rewards a collaborative research approach. A network of PUI ecologists can incorporate closely supervised data collection into undergraduate courses, thereby generating data across spatial gradients to answer crucial questions. These data can offer unprecedented insight into fine-and large-scale spatial processes for publications, resource management, and policy decisions. Undergraduate students benefit from the collaborative research experience as they gain experiential learning in team building, project design, implementation, data collection, and analysis. With appropriate funding, collaborative networks make excellent use of the intellectual and experiential capital of PUI faculty for the benefit of science, pedagogy, and society. © 2011 by American Institute of Biological Sciences. All rights reserved

Reservoir competence of vertebrate hosts for Anaplasma phagocytophilum

Fourteen vertebrate species (10 mammals and 4 birds) were assessed for their ability to transmit Anaplasma phagocytophilum, the bacterium that causes human granulocytic anaplasmosis, to uninfected feeding ixodid ticks. Small mammals were most likely to infect ticks but all species assessed were capable of transmitting the bacterium, in contrast to previous findings

Appendix D. Tri-state study species list.

Tri-state study species list