168 research outputs found
Polymer coated cerium oxide nanoparticles as oxidoreductase-like catalysts
Cerium oxide nanoparticles have been shown to mimic oxidoreductase enzymes by
catalyzing the decomposition of organic substrates and reactive oxygen species.
This mimicry can be found in superoxide radicals and hydrogen peroxides,
harmful molecules produced in oxidative stress asso-ciated diseases. Despite
the fact that nanoparticle functionalization is mandatory in the context of
nanomedicine, the influence of polymer coatings on their enzyme-like catalytic
activity is poorly understood. In this work, six polymer coated cerium oxide
nanoparticles are prepared by associa-tion of 7.8 nm cerium oxide cores with
two poly(sodium acrylate) and four poly(ethylene glycol) (PEG) grafted
copolymers with different terminal or anchoring end groups, such as phosphonic
acids. The superoxide dismutase-, catalase-, peroxidase- and oxidase-like
catalytic activities of the coated nanoparticles were systematically studied.
It is shown that the polymer coatings do not af-fect the superoxide
dismutase-like, impair the catalase-like and oxidase-like and surprisingly
im-proves peroxidase-like catalytic activities of cerium oxide nanoparticles.
It is also demonstrated that the particles coated with the PEG-grafted
copolymers perform better than the poly(acrylic acid) coated ones as
oxidoreductase-like enzymes, a result that confirms the benefit of having
phosphon-ic acids as anchoring groups at the particle surface.Comment: 23 pages, 8 figures, 3 table
Cerium Oxide Nanoparticles Protect Cardiac Progenitor Cells from Oxidative Stress
Cardiac progenitor cells (CPCs) are a promising autologous source of cells for cardiac
regenerative medicine. However, CPC culture in vitro requires the presence of microenvironmental
conditions (a complex array of bioactive substance concentration, mechanostructural
factors, and physicochemical factors) closely mimicking the natural cell surrounding in vivo,
including the capability to uphold reactive oxygen species (ROS) within physiological levels
in vitro. Cerium oxide nanoparticles (nanoceria) are redox-active and could represent a potent
tool to control the oxidative stress in isolated CPCs. Here, we report that 24 h exposure to 5, 10,
and 50 !g/mL of nanoceria did not a!ect cell growth and function in cardiac progenitor cells,
while being able to protect CPCs from H2O2-induced cytotoxicity for at least 7 days, indicating
that nanoceria in an e!ective antioxidant. Therefore, these "ndings con"rm the great
potential of nanoceria for controlling ROS-induced cell damage
Nanoceria Inhibit the Development and Promote the Regression of Pathologic Retinal Neovascularization in the Vldlr Knockout Mouse
Many neurodegenerative diseases are known to occur and progress because of oxidative stress, the presence of reactive oxygen species (ROS) in excess of the cellular defensive capabilities. Age related macular degeneration (AMD), diabetic retinopathy (DR) and inherited retinal degeneration share oxidative stress as a common node upstream of the blinding effects of these diseases. Knockout of the Vldlr gene results in a mouse that develops intraretinal and subretinal neovascular lesions within the first month of age and is an excellent model for a form of AMD called retinal angiomatous proliferation (RAP). Cerium oxide nanoparticles (nanoceria) catalytically scavenge ROS by mimicking the activities of superoxide dismutase and catalase. A single intravitreal injection of nanoceria into the Vldlr-/- eye was shown to inhibit: the rise in ROS in the Vldlr-/- retina, increases in vascular endothelial growth factor (VEGF) in the photoreceptor layer, and the formation of intraretinal and subretinal neovascular lesions. Of more therapeutic interest, injection of nanoceria into older mice (postnatal day 28) resulted in the regression of existing vascular lesions indicating that the pathologic neovessels require the continual production of excessive ROS. Our data demonstrate the unique ability of nanoceria to prevent downstream effects of oxidative stress in vivo and support their therapeutic potential for treatment of neurodegenerative diseases such as AMD and DR
Attaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenching
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Applied Materials and Interfaces copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Pautler, R., Kelly, E. Y., Huang, P.-J. J., Cao, J., Liu, B., & Liu, J. (2013). Attaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenching. ACS Applied Materials & Interfaces, 5(15), 6820–6825. https://doi.org/10.1021/am4018863Cerium oxide nanoparticles (nanoceria) have recently emerged as a nanozyme with oxidase activity. In this work, we present a few important interfacial properties of nanoceria. First, the surface charge of nanoceria can be controlled not only by adjusting pH but also by adsorption of simple inorganic anions. Adsorption of phosphate and citrate gives negatively charged surface over a broad pH range. Second, nanoceria adsorbs DNA via the DNA phosphate backbone in a sequence-independent manner; DNA adsorption inhibits its oxidase activity. Other anionic polymers display much weaker inhibition effects. Adsorption of simple inorganic phosphate does not have the inhibition effect. Third, nanoceria is a quencher for many fluorophores. These discoveries provide an important understanding for further use of nanoceria in biosensor development, materials science, and nanotechnology.University of Waterloo ||
Canadian Foundation for Innovation ||
Natural Sciences and Engineering Research Council ||
Ontario Ministry of Research and Innovation |
Size-dependent stability of ultra-small α-/β-phase tin nanocrystals synthesized by microplasma
Key features of tin, including electronic band structure and opto-electronic properties, are influenced by the crystal structure. Here the authors report a microplasma process for the synthesis of ultra-small tin nanocrystals in which the crystal structure is dependent on crystallite size
Edible bio-based nanostructures: delivery, absorption and potential toxicity
The development of bio-based nanostructures as nanocarriers of bioactive compounds to specific body sites has been presented as a hot topic in food, pharmaceutical and nanotechnology fields. Food and pharmaceutical industries seek to explore the huge potential of these nanostructures, once they can be entirely composed of biocompatible and non-toxic materials. At the same time, they allow the incorporation of lipophilic and hydrophilic bioactive compounds protecting them against degradation, maintaining its active and functional performance. Nevertheless, the physicochemical properties of such structures (e.g., size and charge) could change significantly their behavior in the gastrointestinal (GI) tract. The main challenges in the development of these nanostructures are the proper characterization and understanding of the processes occurring at their surface, when in contact with living systems. This is crucial to understand their delivery and absorption behavior as well as to recognize potential toxicological effects. This review will provide an insight into the recent innovations and challenges in the field of delivery via GI tract using bio-based nanostructures. Also, an overview of the approaches followed to ensure an effective deliver (e.g., avoiding physiological barriers) and to enhance stability and absorptive intestinal uptake of bioactive compounds will be provided. Information about nanostructures potential toxicity and a concise description of the in vitro and in vivo toxicity studies will also be given.Joana T. Martins, Oscar L. Ramos, Ana C. Pinheiro, Ana I. Bourbon, Helder D. Silva and Miguel A. Cerqueira (SFRH/BPD/89992/2012, SFRH/BPD/80766/2011, SFRH/BPD/101181/2014, SFRH/BD/73178/2010, SFRH/BD/81288/2011, and SFRH/BPD/72753/2010, respectively) are the recipients of a fellowship from the Fundacao para a Ciencia e Tecnologia (FCT, POPH-QREN and FSE, Portugal). The authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the project "BioInd-Biotechnology and Bioengineering for improved Industrial and Agro-Food processes," REF.NORTE-07-0124-FEDER-000028, co-funded by the Programa Operacional Regional do Norte (ON.2-O Novo Norte), QREN, FEDER. We also thank to the European Commission: BIOCAPS (316265, FP7/REGPOT-2012-2013.1) and Xunta de Galicia: Agrupamento INBIOMED (2012/273) and Grupo con potencial de crecimiento. The support of EU Cost Action FA1001 is gratefully acknowledged
In situ formation of 1D nanostructures from ceria nanoparticle dispersions by liquid cell TEM irradiation
Deliberate electron irradiation of cerium oxide nanoparticles in water is used to trigger chemical reactions in a liquid cell transmission electron microscope. Formation of nanorods and nanoneedles is observed starting from predominantly octahedral shape nanoparticles. Detailed morphologies found include free-standing needles, needles connected to specific octahedral ceria facets and star-shaped multi-needle patterns. It is found that rod-axis orientations and crystallographic directions are aligned. It is suggested that high ion and radical concentration of radiolysed water dissolves layers of the original CeO2 particles which re-arrange as needles in the direction of energetically preferred facets
Au@Ag Heterogeneous Nanorods as Nanozyme Interfaces with Peroxidase-Like Activity and Their Application for One-Pot Analysis of Glucose at Nearly Neutral pH
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories
- …