32 research outputs found
Scanning dynamic light scattering optical coherence tomography for measurement of high omnidirectional flow velocities
We show scanning dynamic light scattering optical coherence tomography (OCT) omnidirectional flow measurements. Our method improves the velocity measurement limit over conventional correlation-based or phase-resolved Doppler OCT by more than a factor of 2. Our technique is applicable without a-priori knowledge of the flow geometry as our method works both for non-zero Doppler angle and non-ideal scan alignment. In addition, the method improves the particle diffusion coefficient estimation for particles under flow. ImPhys/Computational Imagin
Scanning dynamic light scattering optical coherence tomography for measurement of high omnidirectional flow velocities
We show scanning dynamic light scattering optical coherence tomography (OCT) omnidirectional flow measurements. Our method improves the velocity measurement limit over conventional correlation-based or phase-resolved Doppler OCT by more than a factor of 2. Our technique is applicable without a-priori knowledge of the flow geometry as our method works both for non-zero Doppler angle and non-ideal scan alignment. In addition, the method improves the particle diffusion coefficient estimation for particles under flow.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.ImPhys/Kalkman groupImPhys/Computational Imagin
Sub-diffusion flow velocimetry with number fluctuation optical coherence tomography
We have implemented number fluctuation dynamic light scattering optical coherence tomography (OCT) for measuring extremely slow, sub-diffusion flows of dilute particle suspensions using the second-order autocovariance function. Our method has a lower minimum measurable velocity than conventional correlation-based OCT or phase-resolved Doppler OCT, as the velocity estimation is not affected by the particle diffusion. Similar to non-dilute correlation-based OCT, our technique works for any Doppler angle. With our analysis we can quantitatively determine the concentration of particles under flow. Finally, we demonstrate 2D sub-diffusion flow imaging with a scanning OCT system at high rate by performing number fluctuation correlation analysis on subsequent B-scansImPhys/Kalkman groupImPhys/Computational Imagin
Axial-resolution in depth from focus digital holography
We use digital holography to quantify surface topography of rough objects in full-field. We calculate the variance of the intensity image as a focus metric over a set of reconstruction distances for each pixel, which results in a focus metric curve. The distance where the variance peaks is an estimate for the depth. First we analyze the lateral resolution of this method using the Talbot effect and argue that sub-mm axial resolution is feasible. Then, using a Michelson setup without magnifying optics or lateral scanning we experimentally demonstrate that sub-mm FWHM width of the focus curve can be achieved. This is significantly better than what was previously reported using digital holography and could make this technique useful for characterising objects in art and machine vision.ImPhys/Quantitative Imagin
Sub-diffusion flow velocimetry with number fluctuation optical coherence tomography
We show number fluctuations dynamic light scattering optical coherence tomography (OCT) for measuring extremely slow, sub-diffusion flows of dilute particle suspensions. Our method removes the minimum measurable velocity limitation of conventional correlation-based or phase-resolved Doppler OCT, set by flowing particles’ Brownian motion. Our technique works for any Doppler angle, is applicable to 2D flow imaging with scanning OCT systems and can be used to determine concentration of particles under flow.ImPhys/Kalkman groupImPhys/Computational Imagin
Large-scale high-sensitivity optical diffraction tomography of Zebrafish
In this work we demonstrate large-scale high-sensitivity optical diffraction tomography (ODT) of zebrafish. We make this possible by three improvements. First, we obtain a large field of view while still maintaining a high resolution by using a high magnification over numerical aperture ratio digital holography set-up. With the inclusion of phase shifting we operate close to the optimum magnification over numerical aperture ratio. Second, we decrease the noise in the reconstructed images by implementing off-axis sample placement and numerical focus tracking in combination with the acquisition of a large number of projections. Although both techniques lead to an increase in sensitivity independently, we show that combining them is necessary in order to make optimal use of the potential gain offered by each respective method and obtain a refractive index (RI) sensitivity of 8⋅10 −5 . Third, we optimize the optical clearing procedure to prevent scattering and refraction to occur. We demonstrate our technique by imaging a zebrafish larva over 13 mm 3 field of view with 4 micrometer resolution. Finally, we demonstrate a clinical application of our technique by imaging an entire adult cryoinjured zebrafish heart. ImPhys/Quantitative ImagingImPhys/Imaging Physic
Measuring optical properties of clear and turbid media with broadband spectral interferometry
The group index, ng , group velocity dispersion (GVD), and scattering attenuation coefficient, µs, were measured for dilutions of glycerol, ethanol, and Intralipid 20% with water. Experiments were performed with a supercontinuum laser based Mach–Zehnder spectroscopic interferometry setup for wavelengths between 400 and 930 nm. All optical properties could be retrieved from a single calibrated measurement of the interference spectrum. Scattering attenuation was determined from the envelope of the interference. The group index and GVD were retrieved from the unwrapped spectral phase. It was found that the group indices of glycerol and ethanol dilutions are in accordance with the Lorentz–Lorenz mixing formula. The scattering attenuation matches well to a semi-empirical model based on the Twerksy effective packing fraction.ImPhys/Kalkman groupImPhys/Computational Imagin
Sub-diffusion flow velocimetry with number fluctuation optical coherence tomography
We show number fluctuations dynamic light scattering optical coherence tomography (OCT) for measuring extremely slow, sub-diffusion flows of dilute particle suspensions. Our method removes the minimum measurable velocity limitation of conventional correlation-based or phase-resolved Doppler OCT, set by flowing particles’ Brownian motion. Our technique works for any Doppler angle, is applicable to 2D flow imaging with scanning OCT systems and can be used to determine concentration of particles under flow.ImPhys/Kalkman groupImPhys/Computational Imagin
Polarization contrast optical diffraction tomography
We demonstrate large scale polarization contrast optical diffraction tomography (ODT). In cross-polarized sample arm detection configuration we determine, from the amplitude of the optical wavefield, a relative measure of the birefringence projection. In parallel-polarized sample arm detection configuration we image the conventional phase projection. For off-axis sample placement we observe for polarization contrast ODT, similar as for phase contrast ODT, a strongly reduced noise contribution. In the limit of small birefringence phase shift δ we demonstrate tomographic reconstruction of polarization contrast images into a full 3D image of an optically cleared zebrafish. The polarization contrast ODT reconstruction shows muscular zebrafish tissue, which cannot be visualized in conventional phase contrast ODT. Polarization contrast ODT images of the zebrafish show a much higher signal to noise ratio (SNR) than the corresponding phase contrast images, SNR=73 and SNR=15, respectively.ImPhys/Computational Imagin
Experiment and theory of the complex refractive index of dense colloidal media
The complex refractive index is analyzed by measuring its scattering attenuation µs, group index ng, and group velocity dispersion (GVD) for 100 nm diameter silica nanoparticles dispersed in water. The experiments were performed for wavelengths between 410 nm and 930 nm. The experimental results were compared with different mixing models for the complex refractive index of colloidal suspensions. The group index linearly scaled with the volume fraction both in experiment and for all tested models. It was found that the GVD has a nonlinear dependence on volume fraction in agreement with the coupled dipole model of Parola et al. [J. Chem. Phys. 141, 124902 (2014)] The scattering attenuation is in good agreement with both the coupled dipole model and the low frequency quasi-crystalline approximation [J. Electromagn. Waves Appl. 2, 757 (1988)] that take particle correlations into account. With an iterative fitting procedure of all the data based on both the coupled dipole model and the quasi-crystalline approximation, the refractive index, porosity, and size of the nanoparticles were determined. We determined that the coupled dipole model is in best agreement with the data.ImPhys/Kalkman groupImPhys/Computational Imagin