Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNor-banding and CMA3/DAPI staining

Abstract. The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38–46), Dicerca aenea validiuscula Semenov, 1895 – 2n=20 (18+Xy(p)) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 – 2n=16 (14+Xy(p)) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as well as fluorochrome Chromomycin A(3) (CMA(3)) and DAPI. It is shown that C-positive segments are weakly visible in all four species which indicates a small amount of constitutive heterochromatin (CH). There were no signals after DAPI staining and some positive signals were discovered using CMA(3) staining demonstrating absence of AT-rich DNA and presence of GC-rich clusters of CH. Nucleolus organizing regions (NORs) were revealed using Ag-NOR technique; argentophilic material mostly coincides with positive signals obtained using CMA(3) staining

FIGURES 1–9 in A new species of Protaetia Burmeister, 1842 from Armenia with taxonomic notes on Cetonia floricola fausti Kraatz, 1891 (Coleoptera: Scarabaeidae: Cetoniinae)

FIGURES 1–9. Protaetia (Potosia) species. 1, 2—Protaetia haiastanica new species (paratype, male); 3, 4—Protaetia jelineki (Petrovitz, 1981) (holotype, male); 5, 6—Protaetia caucasica (Kolenati, 1845), Armenia, Gyumri; 7, 8—Protaetia splendidula (Faldermann, 1835),Turkey, Agri; 9—Protaetia fausti (Kraatz, 1891) (junior synonym of P. splendidula) (holotype, female). 1, 3, 5, 7, 9—habitus, dorsal view; 2, 4, 6, 8—habitus, ventral view

On the identity of Cyphonoxia maljuzhenkoi Zaitzev, 1928 (Coleoptera Scarabaeidae: Melolonthinae)

Bezděk, Aleš, Kalashian, Mark, Ghrejyan, Tigran (2019): On the identity of Cyphonoxia maljuzhenkoi Zaitzev, 1928 (Coleoptera Scarabaeidae: Melolonthinae). Zootaxa 4671 (3): 439-445, DOI: https://doi.org/10.11646/zootaxa.4671.3.1

A new Aphodius species of the subgenus Amidorus Mulsant & Rey, from Armenia (Coleoptera, Aphodiidae)

The new species Aphodius (Amidorus) rollandi n. sp. is described from the high mountains of Armenia. This species is illustrated and compared with related taxa. Some etho-ecological data are reported on Amidorus species.Une nouvelle espèce d'Aphodius d'Arménie du sous-genre Amidorus Mulsant & Rey (Coleoptera, Aphodiidae). Des recherches récentes effectuées dans les montagnes d'Arménie ont permis la découverte d'une nouvelle espèce, Aphodius (Amidorus) rollandi n. sp. L'espèce est décrite, illustrée et comparée aux espèces voisines. Quelques données éco-éthologiques sur les Amidorus sont également fournies.Kalashian Mark, Lumaret Jean-Pierre. A new Aphodius species of the subgenus Amidorus Mulsant & Rey, from Armenia (Coleoptera, Aphodiidae). In: Bulletin de la Société entomologique de France, volume 105 (5), décembre 2000. pp. 497-500

A new Aphodius species of the subgenus Amidorus Mulsant & Rey, from Armenia (Coleoptera, Aphodiidae)

The new species Aphodius (Amidorus) rollandi n. sp. is described from the high mountains of Armenia. This species is illustrated and compared with related taxa. Some etho-ecological data are reported on Amidorus species.Une nouvelle espèce d'Aphodius d'Arménie du sous-genre Amidorus Mulsant & Rey (Coleoptera, Aphodiidae). Des recherches récentes effectuées dans les montagnes d'Arménie ont permis la découverte d'une nouvelle espèce, Aphodius (Amidorus) rollandi n. sp. L'espèce est décrite, illustrée et comparée aux espèces voisines. Quelques données éco-éthologiques sur les Amidorus sont également fournies.Kalashian Mark, Lumaret Jean-Pierre. A new Aphodius species of the subgenus Amidorus Mulsant & Rey, from Armenia (Coleoptera, Aphodiidae). In: Bulletin de la Société entomologique de France, volume 105 (5), décembre 2000. pp. 497-500

Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining

The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38–46), Dicerca aenea validiuscula Semenov, 1895 – 2n=20 (18+Xyp) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 – 2n=16 (14+Xyp) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as well as fluorochrome Chromomycin A3 (CMA3) and DAPI. It is shown that C-positive segments are weakly visible in all four species which indicates a small amount of constitutive heterochromatin (CH). There were no signals after DAPI staining and some positive signals were discovered using CMA3 staining demonstrating absence of AT-rich DNA and presence of GC-rich clusters of CH. Nucleolus organizing regions (NORs) were revealed using Ag-NOR technique; argentophilic material mostly coincides with positive signals obtained using CMA3 staining

Data from: Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining

The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38–46), Dicerca aenea validiuscula Semenov, 1895 – 2n=20 (18+Xyp) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 – 2n=16 (14+Xyp) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as well as fluorochrome Chromomycin A3 (CMA3) and DAPI. It is shown that C-positive segments are weakly visible in all four species which indicates a small amount of constitutive heterochromatin (CH). There were no signals after DAPI staining and some positive signals were discovered using CMA3 staining demonstrating absence of AT-rich DNA and presence of GC-rich clusters of CH. Nucleolus organizing regions (NORs) were revealed using Ag-NOR technique; argentophilic material mostly coincides with positive signals obtained using CMA3 staining

Two new species of Sphenoptera from South-West Asia (Coleoptera: Buprestidae: Chrysochroinae)

Kalashian, Mark Yu., Karagyan, Gayane H. (2016): Two new species of Sphenoptera from South-West Asia (Coleoptera: Buprestidae: Chrysochroinae). Acta Entomologica Musei Nationalis Pragae 56 (1): 17-22, DOI: http://doi.org/10.5281/zenodo.450388

New species of Aphanisticus Latreille, 1810 (Coleoptera: Buprestidae) from Sulawesi, Indonesia and from Australia

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