4 research outputs found
Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
Purpose: The use of amniotic membrane has been suggested in the treatment of
infectious keratitis for its intrinsic anti-infective properties probably mediated by its antiinflammatory effects. The aim of this study was to investigate the effect of amniotic
membrane transplantation (AMT) along with ciprofloxacin to cure the primary stages of
Pseudomonas keratitis.
Methods: In total, 28 rabbits were selected and divided in four groups as follows: group
1 as control, group 2 with amniotic membrane, group 3 with ciprofloxacin, and group
4 with amniotic membrane combined with ciprofloxacin. About 0.05 cc suspension of
Pseudomonas aeruginosa, 27853 ATCC was injected into corneal stroma.
Results: The results showed groups of AMT, AMT + ciprofloxacin, and ciprofloxacin had
0% perforation while the control group had 85.6%. Average infiltration of 5.5 mm was
observed in ciprofloxacin group, 5 mm in AMT + ciprofloxacin group, 24 mm in AMT
group, and finally 23.75 mm for control. Amniotic membrane showed to be effective
in prevention of cornea perforation as well as remission of Pseudomonas keratitis.
There was no significant difference between ciprofloxacin groups in comparison with
ciprofloxacin + AMT group. However, regarding the anti-inflammatory effect, the process
of improvement of inflammation in ciprofloxacin + AMT group was faster.
Conclusion: Transplantation of amniotic membrane in the primary stages of
Pseudomonas keratitis treatment remarkably prevents the disease and it can be used
to control its process.
Keywords: Ciprofloxacin; Human Amniotic Membrane; Keratitis; Pseudomonas aeruginosa; Rabbi
Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
Purpose: The use of amniotic membrane has been suggested in the treatment of
infectious keratitis for its intrinsic anti-infective properties probably mediated by its antiinflammatory effects. The aim of this study was to investigate the effect of amniotic
membrane transplantation (AMT) along with ciprofloxacin to cure the primary stages of
Pseudomonas keratitis.
Methods: In total, 28 rabbits were selected and divided in four groups as follows: group
1 as control, group 2 with amniotic membrane, group 3 with ciprofloxacin, and group
4 with amniotic membrane combined with ciprofloxacin. About 0.05 cc suspension of
Pseudomonas aeruginosa, 27853 ATCC was injected into corneal stroma.
Results: The results showed groups of AMT, AMT + ciprofloxacin, and ciprofloxacin had
0% perforation while the control group had 85.6%. Average infiltration of 5.5 mm was
observed in ciprofloxacin group, 5 mm in AMT + ciprofloxacin group, 24 mm in AMT
group, and finally 23.75 mm for control. Amniotic membrane showed to be effective
in prevention of cornea perforation as well as remission of Pseudomonas keratitis.
There was no significant difference between ciprofloxacin groups in comparison with
ciprofloxacin + AMT group. However, regarding the anti-inflammatory effect, the process
of improvement of inflammation in ciprofloxacin + AMT group was faster.
Conclusion: Transplantation of amniotic membrane in the primary stages of
Pseudomonas keratitis treatment remarkably prevents the disease and it can be used
to control its process.
Keywords: Ciprofloxacin; Human Amniotic Membrane; Keratitis; Pseudomonas aeruginosa; Rabbi
Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
Purpose: The use of amniotic membrane has been suggested in the treatment of
infectious keratitis for its intrinsic anti-infective properties probably mediated by its antiinflammatory effects. The aim of this study was to investigate the effect of amniotic
membrane transplantation (AMT) along with ciprofloxacin to cure the primary stages of
Pseudomonas keratitis.
Methods: In total, 28 rabbits were selected and divided in four groups as follows: group
1 as control, group 2 with amniotic membrane, group 3 with ciprofloxacin, and group
4 with amniotic membrane combined with ciprofloxacin. About 0.05 cc suspension of
Pseudomonas aeruginosa, 27853 ATCC was injected into corneal stroma.
Results: The results showed groups of AMT, AMT + ciprofloxacin, and ciprofloxacin had
0% perforation while the control group had 85.6%. Average infiltration of 5.5 mm was
observed in ciprofloxacin group, 5 mm in AMT + ciprofloxacin group, 24 mm in AMT
group, and finally 23.75 mm for control. Amniotic membrane showed to be effective
in prevention of cornea perforation as well as remission of Pseudomonas keratitis.
There was no significant difference between ciprofloxacin groups in comparison with
ciprofloxacin + AMT group. However, regarding the anti-inflammatory effect, the process
of improvement of inflammation in ciprofloxacin + AMT group was faster.
Conclusion: Transplantation of amniotic membrane in the primary stages of
Pseudomonas keratitis treatment remarkably prevents the disease and it can be used
to control its process.
Keywords: Ciprofloxacin; Human Amniotic Membrane; Keratitis; Pseudomonas aeruginosa; Rabbi
Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
Purpose: The use of amniotic membrane has been suggested in the treatment of
infectious keratitis for its intrinsic anti-infective properties probably mediated by its antiinflammatory effects. The aim of this study was to investigate the effect of amniotic
membrane transplantation (AMT) along with ciprofloxacin to cure the primary stages of
Pseudomonas keratitis.
Methods: In total, 28 rabbits were selected and divided in four groups as follows: group
1 as control, group 2 with amniotic membrane, group 3 with ciprofloxacin, and group
4 with amniotic membrane combined with ciprofloxacin. About 0.05 cc suspension of
Pseudomonas aeruginosa, 27853 ATCC was injected into corneal stroma.
Results: The results showed groups of AMT, AMT + ciprofloxacin, and ciprofloxacin had
0% perforation while the control group had 85.6%. Average infiltration of 5.5 mm was
observed in ciprofloxacin group, 5 mm in AMT + ciprofloxacin group, 24 mm in AMT
group, and finally 23.75 mm for control. Amniotic membrane showed to be effective
in prevention of cornea perforation as well as remission of Pseudomonas keratitis.
There was no significant difference between ciprofloxacin groups in comparison with
ciprofloxacin + AMT group. However, regarding the anti-inflammatory effect, the process
of improvement of inflammation in ciprofloxacin + AMT group was faster.
Conclusion: Transplantation of amniotic membrane in the primary stages of
Pseudomonas keratitis treatment remarkably prevents the disease and it can be used
to control its process.
Keywords: Ciprofloxacin; Human Amniotic Membrane; Keratitis; Pseudomonas aeruginosa; Rabbi