22 research outputs found
Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch
Silver birch (Betula pendula) is a pioneer boreal tree that can be induced to flower within 1 year. Its rapid life cycle, small (440-Mb) genome, and advanced germplasm resources make birch an attractive model for forest biotechnology. We assembled and chromosomally anchored the nuclear genome of an inbred B. pendula individual. Gene duplicates from the paleohexaploid event were enriched for transcriptional regulation, whereas tandem duplicates were overrepresented by environmental responses. Population resequencing of 80 individuals showed effective population size crashes at major points of climatic upheaval. Selective sweeps were enriched among polyploid duplicates encoding key developmental and physiological triggering functions, suggesting that local adaptation has tuned the timing of and cross-talk between fundamental plant processes. Variation around the tightly-linked light response genes PHYC and FRS10 correlated with latitude and longitude and temperature, and with precipitation for PHYC. Similar associations characterized the growth-promoting cytokinin response regulator ARR1, and the wood development genes KAK and MED5A.Peer reviewe
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Author Correction: Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch.
In the version of this article initially published, there was a mistake in the calculation of the nucleotide mutation rate per site per generation: 1 Ă 10â9 mutations per site per generation was used, whereas 9.5 Ă 10â9 was correct. This error affects the interpretation of population-size changes over time and their possible correspondence with known geological events, as shown in the original Fig. 4 and supporting discussion in the text, as well as details in the Supplementary Note. Neither the data themselves nor any other results are affected. Figure 4 has been revised accordingly. Images of the original and corrected figure panels are shown in the correction notice
Mycobacterium marinum Causes a Latent Infection that Can Be Reactivated by Gamma Irradiation in Adult Zebrafish
The mechanisms leading to latency and reactivation of human tuberculosis are still unclear, mainly due to the lack of standardized animal models for latent mycobacterial infection. In this longitudinal study of the progression of a mycobacterial disease in adult zebrafish, we show that an experimental intraperitoneal infection with a low dose (~35 bacteria) of Mycobacterium marinum, results in the development of a latent disease in most individuals. The infection is characterized by limited mortality (25%), stable bacterial loads 4 weeks following infection and constant numbers of highly organized granulomas in few target organs. The majority of bacteria are dormant during a latent mycobacterial infection in zebrafish, and can be activated by resuscitation promoting factor ex vivo. In 5â10% of tuberculosis cases in humans, the disease is reactivated usually as a consequence of immune suppression. In our model, we are able to show that reactivation can be efficiently induced in infected zebrafish by Îł-irradiation that transiently depletes granulo/monocyte and lymphocyte pools, as determined by flow cytometry. This immunosuppression causes reactivation of the dormant mycobacterial population and a rapid outgrowth of bacteria, leading to 88% mortality in four weeks. In this study, the adult zebrafish presents itself as a unique non-mammalian vertebrate model for studying the development of latency, regulation of mycobacterial dormancy, as well as reactivation of latent or subclinical tuberculosis. The possibilities for screening for host and pathogen factors affecting the disease progression, and identifying novel therapeutic agents and vaccine targets make this established model especially attractive.Public Library of Science open acces
Intelectin 3 is dispensable for resistance against a mycobacterial infection in zebrafish (Danio rerio)
Abstract
Tuberculosis is a multifactorial bacterial disease, which can be modeled in the zebrafish (Danio rerio). Abdominal cavity infection with Mycobacterium marinum, a close relative of Mycobacterium tuberculosis, leads to a granulomatous disease in adult zebrafish, which replicates the different phases of human tuberculosis, including primary infection, latency and spontaneous reactivation. Here, we have carried out a transcriptional analysis of zebrafish challenged with low-dose of M. marinum, and identified intelectin 3 (itln3) among the highly up-regulated genes. In order to clarify the in vivo significance of Itln3 in immunity, we created nonsense itln3 mutant zebrafish by CRISPR/Cas9 mutagenesis and analyzed the outcome of M. marinum infection in both zebrafish embryos and adult fish. The lack of functional itln3 did not affect survival or the mycobacterial burden in the zebrafish. Furthermore, embryonic survival was not affected when another mycobacterial challenge responsive intelectin, itln1, was silenced using morpholinos either in the WT or itln3 mutant fish. In addition, M. marinum infection in dexamethasone-treated adult zebrafish, which have lowered lymphocyte counts, resulted in similar bacterial burden in both WT fish and homozygous itln3 mutants. Collectively, although itln3 expression is induced upon M. marinum infection in zebrafish, it is dispensable for protective mycobacterial immune response
Zebrafish mortality, the development of bacterial load and the number of lesions have dose-dependent patterns.
<p>Adult zebrafish were i.p. infected with either a low (34±15 cfu) (nâ=â180) or a high dose (2029±709 cfu) (nâ=â104) of <i>M. marinum</i>. (A) Survival was followed for 32 weeks. * P<0.05 (B) The figure shows the average loads for 5 fish (except 32 wk high dose, nâ=â2). Low-dose statistics: * sig. diff. from 1 wk, ** sig. diff. from 1 and 2 wk. High-dose statistics: *** sig. diff. from 1, 2, 8, 11 and 20 wk. Low-dose vs. high-dose statistics: loads at time-points marked with â are sig. diff. (C) By default, 4 individuals per dose were analyzed by Ziehl-Neelsen staining (except 20 wk high dose, nâ=â3) per time-point The gonads, pancreas, liver, muscle, mesentery, spleen, gut and kidney were assessed and the number of organs with visible bacteria was determined. *P<0.05. (D) The total number of granulomas in a sample set for each individual was counted. * P<0.05.</p
Bacterial dose and the presence of functional adaptive immunity define the outcome of mycobacterial infection.
<p>(A) The early cytokine response at 1 d post infection was measured from wt fish infected with a high (2029±709 cfu) or a low (34±15 cfu) dose or injected with sterile PBS buffer (n in each group 10â20). *P<0.05 (B) Wt fish were infected with a high or a low dose or sterile PBS buffer (for early time-points), and rag1 (â/â) fish were infected with a low dose Nos2b expression was measured with q-RT-PCR (n in each group was 9â20/time point). *P<0.05 (C) Fish were infected as in (B) and <i>IFNÎł1â2</i> was measured with q-RT-PCR. *P<0.05. (D) Adult wt and rag1 (â/â) zebrafish were infected with a low dose (nâ=â30) and followed for survival. *P<0.05 (E) Adult wt and rag1 (â/â) fish were infected with a low dose. Average mycobacterial load was measured by qPCR at 2, 4, and 7 wpi (nâ=â10 per time point). *P<0.05.</p
<i>M. marinum</i> induces the formation of granulomas that mature into well-defined structures during an infection.
<p>In fish infected with a low dose (34±15 cfu) of <i>M. marinum</i>, Ziehl-Neelsen staining at 2 wpi commonly reveals areas with free bacteria (C). Some slightly better formed and restricted areas containing bacteria, here referred to as early granulomas, are also seen (A), but as shown in (B) trichrome staining of the adjacent slide, encapsulation around the mycobacterial lesions is absent at the early stage of infection. At 20 weeks, fish that have survived have mature granulomas (DâF) many of which are multicentric surrounded by a fibrous capsule (D&E). (E) Trichrome staining shows the fibrous capsule in blue (F). The amount of bacteria inside granulomas has increased from the earliest time-points.</p
A major part of the mycobacteria are in a dormant state in latent infection.
<p>(A) Parallel dilutions of fresh logarithmic or old plateau phase <i>M. marinum</i> cultures were plated +/â Rpf to show the resuscitating effect of <i>Micrococcus luteus</i> Rpf on dormant <i>M. marinum</i>. (B) Parallel homogenate sample dilutions from low-dose (34±15 cfu) infected fish (wt or rag1 (â/â)) were plated at different time points +/â Rpf to detect dormant mycobacteria. (C) <i>GltA1</i> expression was measured from low-dose infected rag1 (â/â) and wt fish and high-dose infected wt fish and normalized to the total <i>M. marinum</i> load in each fish measured by qPCR. *P<0.05.</p
Gamma irradiation induces reactivation resulting in increased mortality due to uncontrolled growth of mycobacteria.
<p>(AâC) Zebrafish (nâ=â17) with a latent <i>M. marinum</i> infection were irradiated twice with 25 Gy with one month between the irradiations. Twice irradiated, non-infected zebrafish (nâ=â23) as well as zebrafish with a latent infection (nâ=â14) were included as controls. (A) Survival was followed for 30 days after the second dose. *P<0.05. (B) During this period, moribund or recently dead fish were collected 15â22 days after the second radiation dose. Bacterial loads were compared with those of similarly infected, non-irradiated control fish that were collected at the end-point of the experiment. *P<0.05 (C&D) A representative Ziehl-Neelsen stained sample from a reactivated fish showing large numbers of free mycobacteria (purple areas) in the zebrafish body cavity (C). The sides of the body cavity are marked with arrowheads Oâ=âovary, Pâ=âpancreas, Lâ=âliver, Gâ=âgut, Kâ=âkidney. (D) A picture taken with a higher magnification showing individual rods (few examples pointed out with arrows). (E) Four groups of 4 adult zebrafish (1 rag2-gfp, 1 lck-gfp and 2 wild-type groups) were Îł-irradiated with 25 Gy. Similar control groups were left untreated. Kidneys were collected 8 d post irradiation, pooled and analyzed by FCM. FSC-SSC -plots were gated based on cell size and granularity as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002944#ppat.1002944-Traver2" target="_blank">[56]</a> (gates shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002944#ppat.1002944.s003" target="_blank">Figure S3</a>) to assess the effect of irradiation on leukocyte populations. *P<0.05. For further verification of the effect of radiation on lymphocytes, a GFP gate was used for the rag2 and lck groups expressing GFP in B and T cells, or T cells, respectively. (F) Adult non-infected wt zebrafish were irradiated with 25 Gy once (grey bars) (nâ=â3) or twice (nâ=â7) (black bars) with one month between the doses. Leukocyte recovery and re-depletion were assessed by FCM. Non-irradiated fish (nâ=â4) were used as controls. *P<0.05 (G) Fish with a latent infection (nâ=â7) were irradiated twice with 25 Gy with one month between the doses and plated +/â Rpf for 18 d after the second radiation dose. (H) Fish (nâ=â6) with a latent infection were plated +/â Rpf.</p
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