Совершенствование системы внутреннего контроля по оплате труда в бюджетном учреждении на примере БУ РА «Управление социальной поддержки населения Улаганского района»

Объектом исследования является Бюджетное учреждение Республики Алтай "Управление социальной поддержки населения Улаганского района". Целью магистерской диссертации является разработка направлений совершенствования организации системы внутреннего контроля по оплате труда в Бюджетном учреждении Республики Алтай "Управление социальной поддержки населения Улаганского района". В результате исследования была разработана методика внедрения организации системы внутреннего контроля по оплате труда в БУ РА "УСПН Улаганского района".The object of this research is the Budgetary institution of the Altai Republic "Department of social support of the population of the Ulagan district". The purpose of this master's thesis is to develop ways for improving the organization of the internal control system for wages in the Budgetary institution of the Altai Republic "Department of social support of the population of the Ulagan district". As a result of the studies have been formulated a methodology for the implementation of internal control on wages in the Budgetary institution of the Altai Republic "Department of social support of the population of the Ulagan district"

Mechanisms of prion protein assembly into amyloid

The conversion of the alpha-helical, cellular isoform of the prion protein (PrP(C)) to the insoluble, beta-sheet-rich, infectious, disease-causing isoform (PrP(Sc)) is the key event in prion diseases. In an earlier study, several forms of PrP were converted into a fibrillar state by using an in vitro conversion system consisting of low concentrations of SDS and 250 mM NaCl. Here, we characterize the structure of the fibril precursor state, that is, the soluble state under fibrillization conditions. CD spectroscopy, analytical ultracentrifugation, and chemical cross-linking indicate that the precursor state exists in a monomer-dimer equilibrium of partially denatured, alpha-helical PrP, with a well defined contact site of the subunits in the dimer. Using fluorescence with thioflavin T, we monitored and quantitatively described the kinetics of seeded fibril formation, including dependence of the reaction on substrate and seed concentrations. Exponential, seed-enhanced growth can be achieved in homogeneous solution, which can be enhanced by sonication. From these data, we propose a mechanistic model of fibrillization, including the presence of several intermediate structures. These studies also provide a simplified amplification system for prions