49 research outputs found
Multi-decadal survival of an antarctic nematode, Plectus murrayi, in a -20°C stored moss sample
第2回極域科学シンポジウム/第33回極域生物シンポジウム 11月18日(金) 統計数理研究所 3階セミナー室
南極線虫の乾燥耐性遺伝子LEA
第6回極域科学シンポジウム分野横断セッション:[IB2] 地球環境変動の解析と地球生命システム学の構築11月19日(木) 統計数理研究所 セミナー室1(D305
Functional analysis of anti-desiccation proteins from Antarctic nematode, Panagrolaimus davidi, and Japanese anhydrobiotic tardigrade, Ramazzottius varieornatus
第3回極域科学シンポジウム/第34回極域生物シンポジウム 11月27日(火) 国立極地研究所 3階ラウン
Molecular analysis of the cold tolerant Antarctic nematode, Panagrolaimus davidi
Isolated and established in culture from the Antarctic in 1988, the nematode Panagrolaimus davidi has proven to be an ideal model for the study of adaptation to the cold. Not only is it the best-documented example of an organism surviving intracellular freezing but it is also able to undergo cryoprotective dehydration. As part of an ongoing effort to develop a molecular understanding of this remarkable organism, we have assembled both a transcriptome and a set of genomic scaffolds. We provide an overview of the transcriptome and a survey of genes involved in temperature stress. We also explore, in silico, the possibility that P. davidi will be susceptible to an environmental RNAi response, important for further functional studies
Bryosphere within an Antarctic moss pillar
第6回極域科学シンポジウム分野横断セッション:[IB2] 地球環境変動の解析と地球生命システム学の構築11月19日(木) 統計数理研究所 セミナー室1(D305
On the Antacrctic tardigrade Acutuncus antarcticus found near Showa Station
第3回極域科学シンポジウム/第34回極域生物シンポジウム 11月27日(火) 国立極地研究所 3階ラウン
Unsuspected eukaryotic diversity of aquatic moss pillars inhabiting a freshwater lake in East Antarctica, based on 18S rRNA gene analysis
第2回極域科学シンポジウム/第33回極域生物シンポジウム 11月18日(金) 統計数理研究所 3階リフレッシュフロ
Microfloral association for nitrogen cycling in an Antarctic moss pillar inferred from phylogenetic analyses
第3回極域科学シンポジウム/第34回極域生物シンポジウム 11月27日(火) 国立極地研究所 3階ラウン
Comparative genome and transcriptome analyses of the social amoeba Acytostelium subglobosum that accomplishes multicellular development without germ-soma differentiation
Background
Social amoebae are lower eukaryotes that inhabit the soil. They are characterized by the construction of a starvation-induced multicellular fruiting body with a spore ball and supportive stalk. In most species, the stalk is filled with motile stalk cells, as represented by the model organism Dictyostelium discoideum, whose developmental mechanisms have been well characterized. However, in the genus Acytostelium, the stalk is acellular and all aggregated cells become spores. Phylogenetic analyses have shown that it is not an ancestral genus but has lost the ability to undergo cell differentiation.
Results
We performed genome and transcriptome analyses of Acytostelium subglobosum and compared our findings to other available dictyostelid genome data. Although A. subglobosum adopts a qualitatively different developmental program from other dictyostelids, its gene repertoire was largely conserved. Yet, families of polyketide synthase and extracellular matrix proteins have not expanded and a serine protease and ABC transporter B family gene, tagA, and a few other developmental genes are missing in the A. subglobosum lineage. Temporal gene expression patterns are astonishingly dissimilar from those of D. discoideum, and only a limited fraction of the ortholog pairs shared the same expression patterns, so that some signaling cascades for development seem to be disabled in A. subglobosum.
Conclusions
The absence of the ability to undergo cell differentiation in Acytostelium is accompanied by a small change in coding potential and extensive alterations in gene expression patterns