Being a Christian Librarian: A Partial Definition

In this essay, the intent is partially to define the meaning of the phrase, Christian librarian. Because of journal limits on the length of an article, only a partial definition is possible. As literature about Christian librarianship accumulates, I hope a clearer understanding will emerge. Gregory A. Smith’s Christian Librarianship: Essays on the Integration of Faith and Profession (2002) has given a good start to defining Christian librarianship

What Do Meatpackers & Librarians Have in Common? Library Related Injuries and Possible Solutions

This article examines the benefit of exercise to alleviate or prevent injuries common to librarians and library staff. The library literature discusses some interventions such as ergonomics and corrective surgery regarding work-related injuries, but it barely mentions prevention especially as it relates to exercise. Insurance claims filed by library workers rack up thousands of dollars each year. In addition, lost time at work to correct work-related injuries adds much frustration and lost production. Alleviating or eliminating many such occurrences is possible by initiating and maintaining simple exercise programs. Numerous exercises are given that if used conscientiously will curtail the need for corrective medical intervention

What\u27s a Librarian to Do? Literature Review: The Carpal Tunnel Syndrome

Because carpal tunnel syndrome is considered significantly prevalent among library workers, this literature review was begun to check the injury’s prevalence, seriousness, and potential remedies. The literature review quickly showed the topic fraught with difficulties in everything from trying to define the syndrome to considering best treatments for those afflicted. Although library literature was sparse, the literature was replete with good information and advice. There is still much confusion surrounding the topic, however, and additional information including medical literature was sought to clarify and amplify some of the major aspects of carpal tunnel syndrome as it relates to library workers

Defining cellular microenvironments using multiphoton lithography

textTo understand the chemistry of life processes in detail is largely a challenge of resolving them in their native, cellular environment. Cell culture, first developed a century ago, has proven to be an essential tool for reductionist studies of cellular biochemistry and development. However, for the technology of cell culture to move forward and address increasingly complex problems, in vitro environments must be refined to better reflect the cellular environment in vivo. This dissertation work has focused on the development of methods to define cellular microenvironments using the high resolution, 3D capabilities of multiphoton lithography. Here, site-specific photochemistry using multiphoton excitation is applied to the photocrosslinking of proteins, providing the means to organize bioactive species into well-defined 3D microenvironments. Further, conditions have been identified that enable microfabrication to be performed in the presence of cells -- allowing cell outgrowth and motility to be directed in real time. In addition to the intrinsic chemical functionality of microfabricated protein structures, 3D protein matrices are shown to respond mechanically to changes in the chemical environment, enabling new avenues for micro-scale actuation to be explored. Complex 2D and 3D protein photocrosslinking is further facilitated by integrating transparency and automated reflectance photomasks into the fabrication system. These advances could be transformative in efforts to fabricate precise cellular scaffolding that replicates the morphological (and potentially biochemical) features of in vivo tissue microenvironments. Finally, these methods are applied to the study of microorganism behavior with single-cell resolution. Microarchitectures are designed that allow the position and motion of motile bacterial to generate directional microfluidic flow -- providing a foundation to develop micro-scale devices powered by cells.Chemistry and BiochemistryBiochemistr

Combined epiretinal and internal limiting membrane peeling facilitated by high dilution indocyanine green negative staining

We describe the utilization of indocyanine green (ICG) dye to facilitate combined/en bloc removal of epiretinal membranes (ERM) along with internal limiting membranes (ILM). The method utilizes a highly diluted preparation of ICG in dextrose water solvent (D5W). Elimination of fluid air exchange step facilitating staining in the fluid phase and low intensity lighting help minimize potential ICG toxicity. The technique demonstrates how ICG facilitates negative staining of ERMs and how ILM peeling concomitantly can allow complete and efficient ERM removal minimizing surgical time and the necessity for dual or sequential staining

Inertial Focusing of Particles in Curved Micro-channels

Inertial focusing is the migration of particles in flow laterally across a channel into well-defined equilibrium positions. In microfluidic channels, inertial focusing takes advantage of hydrodynamic interactions even at high flow speeds. Particle isolation through inertial focusing is a high throughput method of processing biological samples for point-of-care diagnostics. While photos provide qualitative analyses of inertial focusing, we desired quantitative characterization of these systems. In this study, we ran flow experiments, first with fluorescent polystyrene beads and later with cells in solution, through curved micro-channels at controlled rates using a syringe pump. Our results from polystyrene bead experiments confirmed previous studies on flow through curved micro-channels, in which particles are focused along both sides of the channel at low flow rates and transition towards the center of the channel as the flow rate increases. FWHM analysis also showed that the streamline width is minimized at an intermediate flow rate, indicating inertial focusing is optimized under that condition. As this method of analysis was confirmed with polystyrene beads, we further used this analysis method to characterize the focusing of cells in solution. To maximize both throughput and purity, microfluidic devices must be designed to operate at the highest flow rate at which effective separation from bulk fluid can occur. The device presented in this report indeed isolates the desired target cells to be studied in downstream characterization.http://deepblue.lib.umich.edu/bitstream/2027.42/169578/1/Honors_Capstone_Anna_Kaehr.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/169578/2/Kaehr_Anna_Capstone_Poster.pptxhttp://deepblue.lib.umich.edu/bitstream/2027.42/169578/3/Capstone_Presentation_Video_Anna_Kaehr.mp

Probing Prokaryotic Social Behaviors with Bacterial “Lobster Traps”

Bacteria are social organisms that display distinct behaviors/phenotypes when present in groups. These behaviors include the abilities to construct antibiotic-resistant sessile biofilm communities and to communicate with small signaling molecules (quorum sensing [QS]). Our understanding of biofilms and QS arises primarily from in vitro studies of bacterial communities containing large numbers of cells, often greater than 108 bacteria; however, in nature, bacteria often reside in dense clusters (aggregates) consisting of significantly fewer cells. Indeed, bacterial clusters containing 101 to 105 cells are important for transmission of many bacterial pathogens. Here, we describe a versatile strategy for conducting mechanistic studies to interrogate the molecular processes controlling antibiotic resistance and QS-mediated virulence factor production in high-density bacterial clusters. This strategy involves enclosing a single bacterium within three-dimensional picoliter-scale microcavities (referred to as bacterial “lobster traps”) defined by walls that are permeable to nutrients, waste products, and other bioactive small molecules. Within these traps, bacteria divide normally into extremely dense (1012 cells/ml) clonal populations with final population sizes similar to that observed in naturally occurring bacterial clusters. Using these traps, we provide strong evidence that within low-cell-number/high-density bacterial clusters, QS is modulated not only by bacterial density but also by population size and flow rate of the surrounding medium. We also demonstrate that antibiotic resistance develops as cell density increases, with as few as ~150 confined bacteria exhibiting an antibiotic-resistant phenotype similar to biofilm bacteria. Together, these findings provide key insights into clinically relevant phenotypes in low-cell-number/high-density bacterial populations