Effect of vitamin K1 supplementation on left colon healing in rats with extrahepatic biliary obstruction

PURPOSE: To evaluate the effects of vitamin K1 on wound healing in the left colon of rats with experimental biliary obstruction.METHODS: Sixteen male rats, divided into four groups of four animals each (L, M, LK, and MK), underwent colostomy followed by bowel suture in the left colon. Seven days before, animals in the L and LK groups had undergone common bile duct ligation. The animals in groups MK and LK received vitamin K1 supplementation. On day 7 after bowel suture, repeat laparotomy was performed for evaluation of colonic healing by burst pressure measurement and collection of samples for histopathological analysis. Changes in body weight were evaluated in the four groups.RESULTS:Weight loss was lower in animals supplemented with vitamin K. No significant differences were observed in burst pressure among the four groups (p>0.05). Histological analysis showed more hemorrhage and congestion in the biliary obstruction groups. Supplemented animals exhibited increased collagen formation and less edema and abscess formation.CONCLUSION:Vitamin K supplementation attenuated weight loss and improved colonic wound healing in rats

The Prevalence of Iron Deficiency Anemia in Primary Antiphospholipid Syndrome

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Objectives: The aim of this study was to evaluate the prevalence of subclinical and clinical iron deficiency with iron deficiency anemia in primary antiphospholipid syndrome (PAPS). Patients and methods: The study was comprised of 29 PAPS patients and 29 healthy controls matched for age, gender, and socioeconomic status. Participants received iron, folic acid, vitamin B12, and vitamin C. A battery of tests was performed to determine the iron storage. The mean disease duration was 70 +/- 51.3 months in the patient group. Results: Iron storage depletion was observed in 10.3% of the individuals in both groups (p= 0.5). Iron deficient erythropoiesis (IDE) was observed in only three PAPS patients (10.3%) (p<0.001). Iron deficiency anemia (IDA) was more common in the PAPS patients compared to controls (48.2% vs. 10.3%, respectively; p=0.009). The mean iron levels were significantly lower in the PAPS group than the controls (75.5 vs. 95.8, respectively; p=0.03). Red cell distribution width-coefficient of variation (RDW-CV) (14.9 vs. 13.2; p= 0.02) and red cell distribution width-standard deviation (RDW-SD) (46.7 vs. 40.5; p= 0.009) were significantly increased in the patient group. The folic acid and vitamin C levels were lower in the PAPS group than the control group (p<0.05). Conclusion: This study showed for the first time that PAPS patients have a higher incidence of IDA and IDE compared to healthy controls. This can be attributed to inadequate ingestion of folic acid and vitamin C.28296100Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Dr. Carvalho: Federico FoundationFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP [FAPESP 2008/02917-0, 2009/06049-6]CNPq [300447/2009-4]CNPq [300665/2009-1

The Prevalence Of Iron Deficiency Anemia In Primary Antiphospholipid Syndrome [primer Antifosfolipid Sendromda Demir Eksikliǧi Anemisinin Prevalansi{dotless}]

Objectives: The aim of this study was to evaluate the prevalence of subclinical and clinical iron deficiency with iron deficiency anemia in primary antiphospholipid syndrome (PAPS). Patients and methods: The study was comprised of 29 PAPS patients and 29 healthy controls matched for age, gender, and socioeconomic status. Participants received iron, folic acid, vitamin B12, and vitamin C. A battery of tests was performed to determine the iron storage. The mean disease duration was 70±51.3 months in the patient group. Results: Iron storage depletion was observed in 10.3% of the individuals in both groups (p=0.5). Iron deficient erythropoiesis (IDE) was observed in only three PAPS patients (10.3%) (p<0.001). Iron deficiency anemia (IDA) was more common in the PAPS patients compared to controls (48.2% vs. 10.3%, respectively; p=0.009). The mean iron levels were significantly lower in the PAPS group than the controls (75.5 vs. 95.8, respectively; p=0.03). Red cell distribution width-coefficient of variation (RDW-CV) (14.9 vs. 13.2; p=0.02) and red cell distribution widthstandard deviation (RDW-SD) (46.7 vs. 40.5; p=0.009) were significantly increased in the patient group. The folic acid and vitamin C levels were lower in the PAPS group than the control group (p<0.05). Conclusion: This study showed for the first time that PAPS patients have a higher incidence of IDA and IDE compared to healthy controls. This can be attributed to inadequate ingestion of folic acid and vitamin C. © 2013 Turkish League Against Rheumatism. All rights reserved.28296100Uthman, I., Godeau, B., Taher, A., Khamashta, M., The hematologic manifestations of the antiphospholipid syndrome (2008) Blood Rev, 22, pp. 187-194. , doi: 10.1016/j. blre.2008.03.005Khamashta, M.A., Bertolaccini, M.L., Hughes, G.R., Antiphospholipid (Hughes) syndrome (2004) Autoimmunity, 37, pp. 309-312Vermylen, J., Carreras, L.O., Arnout, J., Attempts to make sense of the antiphospholipid syndrome (2007) J Thromb Haemost, 5, pp. 1-4. , Epub 2006Cook, J.D., Diagnosis and management of irondeficiency anaemia (2005) Best Pract Res Clin Haematol, 18, pp. 319-332Tincani, A., Taraborelli, M., Cattaneo, R., Antiphospholipid antibodies and malignancies (2010) Autoimmun Rev, 9, pp. 200-202. , doi: 10.1016/j.autrev.2009.04.001Miyakis, S., Lockshin, M.D., Atsumi, T., Branch, D.W., Brey, R.L., Cervera, R., International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS) (2006) J Thromb Haemost, 4, pp. 295-306Miyakis, S., Lockshin, M.D., Atsumi, T., Branch, D.W., Brey, R.L., Cervera, R., International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS) (2006) J Thromb Haemost, 4, pp. 295-306(1998) Dietary Reference Intakes for thiamin, riboflavin, niacin, vitamin B6, folate, vitamin B12, pantothenic acid, biotin, and choline., , http://www.nal.usda.gov/fnic/DRI//DRI_Thiamin/thiamin_full_report.pdf, National Agricultural Library. United States Departament of Agriculture. Food and Nutrition Information CenterAvailable from(2000) Dietary Reference Intakes for vitamin C, vitamin E, selenium, and carotenoids., , http://www.nal.usda.gov/fnic/DRI/DRI_Vitamin_C/vitamin_c_full_report.pdf, National Agricultural Library. United States Departament of Agriculture. Food and Nutrition Information CenterAvailable from(2001) Dietary Reference Intakes for vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc., , http://www.nal.usda.gov/fnic/DRI//DRI_Vitamin_A/vitamin_a_full_report.pdf, National Agricultural Library. United States Departament of Agriculture. Food and Nutrition Information CenterAvailable from(2002) Departamento de Informática em Saúde., , Nutwin-Programa de Apoio à Nutrição. DIS-UNIFESP/EPM. Universidade Federal de São Paulo-Versão 1.5Cook, J., The nutritional assessment of iron status (1999) Arch Latinoam Nutr, 49, pp. 11S-14SPasricha, S.R., Flecknoe-Brown, S.C., Allen, K.J., Gibson, P.R., McMahon, L.P., Olynyk, J.K., Diagnosis and management of iron deficiency anaemia: A clinical update (2010) Med J Aust, 193, pp. 525-532Killip, S., Bennett, J.M., Chambers, M.D., Iron deficiency anemia (2007) Am Fam Physician, 75, pp. 671-678Cook, J.D., Diagnosis and management of iron-deficiency anaemia (2005) Best Pract Res Clin Haematol, 18, pp. 319-33

Primary antiphospholipid syndrome in premenopausal women: low vitamin D, high fat mass and maintained bone mineral mass

The aim of this study was to analyze vitamin D levels and their association with bone mineral density and body composition in primary antiphospholipid syndrome. For this cross-sectional study 23 premenopausal women with primary antiphospholipid syndrome (Sapporo criteria) and 23 age- and race-matched healthy controls were enrolled. Demographic, anthropometric, clinical and laboratorial data were collected using clinical interview and chart review. Serum 25-hydroxyvitamin D levels, parathormone, calcium and 24-hour urinary calcium were evaluated in all subjects. Bone mineral density and body composition were studied by dual X-ray absorptiometry. The mean age of patients and controls was 33 years. Weight (75.61 [20.73] vs. 63.14 [7.34] kg, p=0.009), body mass index (29.57 [7.17] vs. 25.35 [3.37] kg, p=0.014) and caloric ingestion (2493 [1005.6] vs. 1990 [384.1] kcal/day, p=0.03) were higher in PAPS than controls. All PAPS were under oral anticoagulant with INR within therapeutic range. Interestingly, biochemical bone parameters revealed lower levels of 25-hydroxyvitamin D [21.64 (11.26) vs. 28.59 (10.67) mg/dl, p=0.039], serum calcium [9.04 (0.46) vs. 9.3 (0.46) mg/dl, p=0.013] and 24-hour urinary calcium [106.55 (83.71) vs. 172.92 (119.05) mg/d, p=0.027] in patients than in controls. Supporting these findings, parathormone levels were higher in primary antiphospholipid syndrome than in controls [64.82 (37.83) vs. 44.53 (19.62) pg/ml, p=0.028]. The analysis of osteoporosis risk factors revealed that the two groups were comparable (p>0.05). Lumbar spine, femoral neck, total femur and whole body bone mineral density were similar in both groups (p>0.05). Higher fat mass [28.51 (12.93) vs. 20.01 (4.68) kg, p=0.005] and higher percentage of fat [36.08 (7.37) vs. 31.23 (4.64)%, p=0.010] were observed in PAPS in comparison with controls; although no difference was seen regarding lean mass. In summary, low vitamin D in primary antiphospholipid syndrome could be secondary to higher weight and fat mass herein observed most likely due to adipocyte sequestration. This weight gain may also justify the maintenance of bone mineral density even with altered biochemical bone parameters. Lupus (2010) 19, 1302-1306.Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)[300559/2009-7]Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)[300665/2009-1]Wilhelm Agricola Federico Foundatio