Poetics of Book “Journey of Russian Imperial Sloop “Diana” from Kronstadt to Kamchatka ...” by V. M. Golovnin: Features of Documentary and Fiction Prose

Observations are presented on the features of the documentary and artistic organization of the book by V. M. Golovnin “Journey of Russian Imperial Sloop “Diana” from Kronstadt to Kamchatka...” (1819). Such issues as the thematic content of “Journey...”, the role of the author’s worldview in creating a single artistic whole, and the features of the aesthetic organization of documentary material are considered. The significance of the study is seen in the need to update the memory of the personality and literary work of V. M. Golovnin, an outstanding figure of Russian culture at the beginning of the 19th century. The presented material will allow supplementing with new facts the picture of the genre and style genesis of documentary and artistic genres in the Russian historical and literary process of the early 19th century. The relevance of the study is determined by the attention of modern literary criticism to the poetics of documentary and artistic genres. The novelty of the research is seen in the appeal to a little-known work of Russian literature of the first third of the 19th century. It is reported that the author showed the maritime way of life with its regulations, traditions, experience of intercultural communication. The work is considered in the main thematic lines: everyday life of a round-the-world sea expedition, seascape, battle studies, ethnographic sketches. It is noted that a value-semantic principle is revealed behind the empirical material, which gives the narrative artistic completeness and deeply brings Golovnin's “Journey ...” with the tradition of Russian classical literature

Analysis of the effectiveness of callusogenesis on media developed on the basis of the RZ medium

There is still no consensus on the composition of nutrient media for various genotypes, and the improvement of their composition is still an urgent problem, the solution of which can significantly speed up and facilitate the breeding process, both in traditional and heterotic breeding. In this regard, the main goal of the study was to compile new variants of nutrient media and establish the relationship between changes in the components of the medium and the efficiency of callus formation. Previously, we showed the advantage of the RZ nutrient medium (Raina, Zapata. 1997) over the N nutrient medium (Nitsch, Nitsch 1969) for domestic varieties and hybrids, but to further increase the efficiency of callusogenesis, 5 variants based on the RZ nutrient medium were developed. The nutrient medium R1 was characterized by a reduced content of CaCl2·2H2O by almost three times, however, the results obtained on it were significantly better than on the initial nutrient medium RZ

Salinity modulates thermotolerance, energy metabolism and stress response in amphipods Gammarus lacustris

Temperature and salinity are important abiotic factors for aquatic invertebrates. We investigated the influence of different salinity regimes on thermotolerance, energy metabolism and cellular stress defense mechanisms in amphipods Gammarus lacustris Sars from two populations. We exposed amphipods to different thermal scenarios and determined their survival as well as activity of major antioxidant enzymes (peroxidase, catalase, glutathione S-transferase) and parameters of energy metabolism (content of glucose, glycogen, ATP, ADP, AMP and lactate). Amphipods from a freshwater population were more sensitive to the thermal challenge, showing higher mortality during acute and gradual temperature change compared to their counterparts from a saline lake. A more thermotolerant population from a saline lake had high activity of antioxidant enzymes. The energy limitations of the freshwater population (indicated by low baseline glucose levels, downward shift of the critical temperature of aerobic metabolism and inability to maintain steady-state ATP levels during warming) was observed, possibly reflecting a trade-off between the energy demands for osmoregulation under the hypo-osmotic condition of a freshwater environment and protection against temperature stress

Overexpression of Akt1 Enhances Adipogenesis and Leads to Lipoma Formation in Zebrafish

<div><h3>Background</h3><p>Obesity is a complex, multifactorial disorder influenced by the interaction of genetic, epigenetic, and environmental factors. Obesity increases the risk of contracting many chronic diseases or metabolic syndrome. Researchers have established several mammalian models of obesity to study its underlying mechanism. However, a lower vertebrate model for conveniently performing drug screening against obesity remains elusive. The specific aim of this study was to create a zebrafish obesity model by over expressing the insulin signaling hub of the <em>Akt1</em> gene.</p> <h3>Methodology/Principal Findings</h3><p><em>Skin oncogenic transformation screening shows that a stable zebrafish transgenic of Tg(krt4Hsa.myrAkt1</em>)^cy18 displays severely obese phenotypes at the adult stage. In Tg(<em>krt4:Hsa.myrAkt1</em>)^cy18, the expression of exogenous human constitutively active Akt1 (myrAkt1) can activate endogenous downstream targets of mTOR, GSK-3α/β, and 70S6K. During the embryonic to larval transitory phase, the specific over expression of myrAkt1 in skin can promote hypertrophic and hyperplastic growth. From 21 hour post-fertilization (hpf) onwards, myrAkt1 transgene was ectopically expressed in several mesenchymal derived tissues. This may be the result of the integration position effect. Tg(<em>krt4:Hsa.myrAkt1</em>)^cy18 caused a rapid increase of body weight, hyperplastic growth of adipocytes, abnormal accumulation of fat tissues, and blood glucose intolerance at the adult stage. Real-time RT-PCR analysis showed the majority of key genes on regulating adipogenesis, adipocytokine, and inflammation are highly upregulated in Tg(<em>krt4:Hsa.myrAkt1</em>)^cy18. In contrast, the myogenesis- and skeletogenesis-related gene transcripts are significantly downregulated in Tg(<em>krt4:Hsa.myrAkt1</em>)^cy18, suggesting that excess adipocyte differentiation occurs at the expense of other mesenchymal derived tissues.</p> <h3>Conclusion/Significance</h3><p>Collectively, the findings of this study provide direct evidence that Akt1 signaling plays an important role in balancing normal levels of fat tissue in vivo. The obese zebrafish examined in this study could be a new powerful model to screen novel drugs for the treatment of human obesity.</p> </div

Proton-Assisted Amino Acid Transporter PAT1 Complexes with Rag GTPases and Activates TORC1 on Late Endosomal and Lysosomal Membranes

Mammalian Target of Rapamycin Complex 1 (mTORC1) is activated by growth factor-regulated phosphoinositide 3-kinase (PI3K)/Akt/Rheb signalling and extracellular amino acids (AAs) to promote growth and proliferation. These AAs induce translocation of mTOR to late endosomes and lysosomes (LELs), subsequent activation via mechanisms involving the presence of intralumenal AAs, and interaction between mTORC1 and a multiprotein assembly containing Rag GTPases and the heterotrimeric Ragulator complex. However, the mechanisms by which AAs control these different aspects of mTORC1 activation are not well understood. We have recently shown that intracellular Proton-assisted Amino acid Transporter 1 (PAT1)/SLC36A1 is an essential mediator of AA-dependent mTORC1 activation. Here we demonstrate in Human Embryonic Kidney (HEK-293) cells that PAT1 is primarily located on LELs, physically interacts with the Rag GTPases and is required for normal AA-dependent mTOR relocalisation. We also use the powerful in vivo genetic methodologies available in Drosophila to investigate the regulation of the PAT1/Rag/Ragulator complex. We show that GFP-tagged PATs reside at both the cell surface and LELs in vivo, mirroring PAT1 distribution in several normal mammalian cell types. Elevated PI3K/Akt/Rheb signalling increases intracellular levels of PATs and synergistically enhances PAT-induced growth via a mechanism requiring endocytosis. In light of the recent identification of the vacuolar H+-ATPase as another Rag-interacting component, we propose a model in which PATs function as part of an AA-sensing engine that drives mTORC1 activation from LEL compartments

Analysis of the effectiveness of callusogenesis on media developed on the basis of the RZ medium

There is still no consensus on the composition of nutrient media for various genotypes, and the improvement of their composition is still an urgent problem, the solution of which can significantly speed up and facilitate the breeding process, both in traditional and heterotic breeding. In this regard, the main goal of the study was to compile new variants of nutrient media and establish the relationship between changes in the components of the medium and the efficiency of callus formation. Previously, we showed the advantage of the RZ nutrient medium (Raina, Zapata. 1997) over the N nutrient medium (Nitsch, Nitsch 1969) for domestic varieties and hybrids, but to further increase the efficiency of callusogenesis, 5 variants based on the RZ nutrient medium were developed. The nutrient medium R1 was characterized by a reduced content of CaCl2·2H2O by almost three times, however, the results obtained on it were significantly better than on the initial nutrient medium RZ