Photoelectrochemical Detection of Dengue-Related Oligonucleotide Sequence Using Anthocyanin as an Intercalating Agent and Electrochromic Material

World Health Organization (WHO) presupposes a confirmation of dengue virus infection diagnosis with two criteria, i.e. clinical and laboratory criteria. One of the basic methods used by most laboratories to diagnose dengue virus is to detect oligonucleotide sequence using a DNA amplification technique. In this research, the measurement of denguerelated oligonucleotide was conducted by photoelectrochemical method. The presence of oligonucleotide sequence in target DNA can be detected by DNA probe that is immobilized on TiO2 electrode. The DNA hybrid is then bound to electrochromic substance like anthocyanin that generates current when it is subjected to light. The photocurrent is directly proportional to the number of target DNA. The aim of this research is to obtain photoelectrochemical system that has sensitivity and high responsiveness toward the change in oligonucleotide concentration, especially the applicability of anthocyanin as a electrochromic substance and intercalating agent. Linearity (R2) generated from the change of current in response to concentration changes of target DNA (in the concentration range of 0.75–3.00 nM) is 0.9611. Thus, this method has the potential to be developed to detect the presence of dengue virus in biological sample

One-loop contributions of charginos and neutralinos to W-pair production in E+ E- collisions

We study the one-loop effects of charginos and neutralinos on the helicity amplitudes for \eeww in the minimal supersymmetric standard model. The calculation is tested by using two methods. First, the sum rule for the form factors between \eeww and the process where the external $W^\pm$ bosons are replaced by the corresponding Goldstone bosons $\omega^\pm$ is employed to test the analytic expression and the accuracy of the numerical program. Second, the decoupling property in the large mass limit is used to test the overall normalization of the amplitudes. These two tests are most effectively carried out when the amplitudes are expanded in terms of the modified minimal subtraction ($\bar{\rm MS}$) couplings of the standard model. The resulting perturbation expansion is valid at collider energies below and around the threshold of the light supersymmetric particles. We find that the corrections to the cross section of the longitudinally polarized $W$-pair production can be as large as -1.4% at the threshold of the light chargino-pair production for large scattering angles. We also study the effects of the CP-violating phase in the chargino and neutralino sectors on the helicity amplitudes. We find that the resulting CP-violating asymmetries can be at most 0.1%.Comment: 30 pages, 25 figures, Final verision, To appear in Physical Review D, Several sentences are improve

On Dynamics of Spinning Structures

This paper provides details of developments pertaining to vibration analysis of gyroscopic systems, that involves a finite element structural discretization followed by the solution of the resulting matrix eigenvalue problem by a progressive, accelerated simultaneous iteration technique. Thus Coriolis, centrifugal and geometrical stiffness matrices are derived for shell and line elements, followed by the eigensolution details as well as solution of representative problems that demonstrates the efficacy of the currently developed numerical procedures and tools

Classification of Several Skin Cancer Types Based on Autofluorescence Intensity of Visible Light to Near Infrared Ratio

Skin cancer is a Malignant growth on the skin caused by many factors. The most common skin cancers are Basal Cell Cancer (BCC) and Squamous Cell Cancer (SCC). This research uses a discriminant analysis to classify some tissues of skin cancer based on criterion number of independent variables. An independent variable is variation of excitation light sources (LED lamp), filters, and sensors to measure autofluorescence intensity (IAF) of visible light to near infrared (VIS/NIR) ratio of paraffin embedded tissue biopsy from BCC, SCC, and Lipoma. From the result of discriminant analysis, it is known that the discriminant function is determined by 4 (four) independent variables i.e., blue LED-red filter, blue LED-yellow filter, UV LED-blue filter, and UV LED-yellow filter. The accuracy of discriminant in classifying the analysis of three skin cancer tissues is 100%

Corrosion study of pipeline material for seabed sediment in tropical climate

Corrosive environments such as marine sediments can cause corrosion to steel pipelines at any time when certain conditions are met. Seabed sediment could cause severe corrosion damage due to its corrosiveness to the pipelines buried under it. Many consequences could take place in case if there is incident in oil/gas pipelines. Successfully identifying elements of corrosion in marine sediment would enhance the future of steel structure protection and monitoring systems. This article focuses on the behaviour of corrosion rate of steel located near shore environment and the aim is to determine the effect of sediment on corrosion of steel. To investigate that, simulated near shore sediment conditions have been used where the steel coupons buried in sediments which have different characteristics. Weight loss technique has been implemented to determine the weight loss rate of the steel specimens. Based on the results of this study, metal weight loss increases as the duration of exposure to seabed sediment environment become longer. The sea sediment simulated condition has given significant levels of corrosion. Conclusively, the corrosion rate of steel in seabed sediment located in tropical region is complicated and further studies are suggested

CP-odd Phase Correlations and Electric Dipole Moments

We revisit the constraints imposed by electric dipole moments (EDMs) of nucleons and heavy atoms on new CP-violating sources within supersymmetric theories. We point out that certain two-loop renormalization group corrections induce significant mixing between the basis-invariant CP-odd phases. In the framework of the constrained minimal supersymmetric standard model (CMSSM), the CP-odd invariant related to the soft trilinear A-phase at the GUT scale, theta_A, induces non-trivial and distinct CP-odd phases for the three gaugino masses at the weak scale. The latter give one-loop contributions to EDMs enhanced by tan beta, and can provide the dominant contribution to the electron EDM induced by theta_A. We perform a detailed analysis of the EDM constraints within the CMSSM, exhibiting the reach, in terms of sparticle spectra, which may be obtained assuming generic phases, as well as the limits on the CP-odd phases for some specific parameter points where detailed phenomenological studies are available. We also illustrate how this reach will expand with results from the next generation of experiments which are currently in development.Comment: 31 pages, 21 eps figures; v2: additional remarks on 2-loop threshold corrections and references added; v3: typos corrected, to appear in Phys. Rev.

One-loop Neutron Electric Dipole Moment from Supersymmetry without R-parity

We present a detailed analysis together with exact numerical calculations on one-loop contributions to neutron electric dipole moment from supersymmetry without R-parity, focusing on the gluino, chargino, and neutralino contributions. Apart from the neglected family mixing among quarks, complete formulae are given for the various contributions, through the quark dipole operators, to which the present study is restricted. We discuss the structure and main features of the R-parity violating contributions and the interplay between the R-parity conserving and violating parameters. In particular, the parameter combination $\mu_i^*\lambda^{\prime}_{i11}$, under the optimal parametrization adopted, is shown to be solely responsible for the R-parity violating contributions in the supersymmetric loop diagrams. While $\mu_i^*\lambda^{\prime}_{i11}$ could bear a complex phase, the latter is not necessary to have a R-parity violating contribution.Comment: 43 pages Revtex with 15 eps- and 4 ps- figure files incoporated; proofread version to be published in Phys. Rev.

Performance of metakaolin based geopolymer concrete at elevated temperature

Due to the carbon dioxide emission arising from the production of cement, alternative concrete that is environmentally friendly such as metakaolin geopolymer concrete have been developed. However, the performance of metakaolin based geopolymer concrete (MKGC) when exposed to aggressive environment particularly elevated temperature has not been investigated. Therefore, this paper assessed the performance of MKGC exposed to elevated temperatures. MKGC cube specimens of grade 25 were produced using a mix ratio of 1:1.58:3.71.After preparing the specimens, they were placed in an electric oven at a temperature of 60oC for 24 hours. Thereafter, the specimens were stored in the laboratory at ambient temperature for 28 days. The specimens were then exposed to elevated temperatures of 200, 400, 600 and 800oC. After exposure to elevated temperatures, the MKGC specimens were subjected to compressive strength, water absorption and abrasion resistance tests. Results show that at 600 and 800oC, the MKGC lost a compressive strength of 59.69% and 71.71% respectively. Higher water absorption and lower abrasion resistance were also observed. Keywords: Cement, Compressive Strength, Metakaolin Concrete, Elevated Temperature

Mechanical and Thermal Properties of Polyurethane-Palm Fronds Ash Composites

The aim of the article is to study the influence of environmentally friendly palm frond ash on the mechanical and thermal properties of polyurethane used as filler. Various weight filler ratios with particle sizes around (125 μm) were examined and characterized using elongation, tensile strength, Young's modulus, compressive strength, average burning time, and infrared spectroscopy. The results showed that the addition of 20 wt.% palm frond ash powder significantly improved the hardness mixture by about 2.83 MPa. In addition to that, the highest value of the compressive strength of the polymer with the additive was recorded at 10 wt.%. Also, the most excellent value of Young’s modulus was 2 MPa at a ratio of 50 wt. %, as was the average burning time of about 33 sec. The mechanical properties of polyurethane were affected by adding palm frond ash, which increases the tensile and compressive strengths, making it suitable for use in many applications. Moreover, the environmentally friendly material reflects the benefits of waste recycling. The addition of filler affects the morphology and strengthens the brittleness. Additionally, the use of fly ash from palm frond combustion in the technology of polyurethane materials complies. Partial replacement of petrochemical components with waste filler also reduces the total energy consumption in producing PU composites. &nbsp