The Role of Differentially Expressed miRNAs and Potential miRNA-mRNA Regulatory Network in Prostate Cancer Progression and Metastasis

Purpose: Aberrant expression of microRNAs (miRNAs) has been discovered in prostate cancer progression however their function is not well understood, thereby further investigation is required to understand the importance of underlying mechanisms and their involvement in multiple signaling pathways, as well as their potential as therapeutic targets. In this study the role and expression levels of three miRNAs were evaluated: miR-21, miR-221 and miR-200c in different prostate cancer cell lines. In addition, based on the latest studies on miRNAs function, their association with other target genes and molecules were analyzed using bioinformatic tools. Methods: Three PCa cell lines PC3, LNCaP and VCaP and normal prostate epithelial cell line PNT1A were screened for miRNA expression levels using qPCR. miRNA target genes and their association with signaling pathways were analyzed through several Network and pathway analysis online tools. Findings: Upregulation of miR-21 and miR-221 was observed in PC3 and VCaP prostate cancer cells, respectively. According to KEGG analysis, we found that Hippo signaling pathway and cytokine-cytokine receptor interactions were affected by miR-21 while miR-221 would interfere with ECM-receptor interaction, Fatty acid elongation and Huntington disease molecular networks. Exposure of PC3 cells to TGF-β (10 µM) caused upregulation of miR-21 with the evidence with increased invasion potential. Discussion and Conclusion: miRNAs could regulate several genes in multiple signaling pathways. Here, we demonstrated that in a panel of PCa cell lines, both mir-21 and miR-221 expressions were upregulated. miR-21 may be a dignostic and prognosticbiomarker for PCa

Introduction to this special issue on HCI and games

Games have been part of human–computer interaction (HCI) research since the first CHI conference in 1982. At that gathering, Tom Malone, then at Xerox PARC, presented insights from the study of computer games to motivate a set of design principles for “enjoyable” user interfaces (Malone, 1982). Over the ensuing years, games-related HCI research has steadily grown as a subarea of CHI (e.g. Keeker, Pagulayan, Sykes, & Lazzaro, 2004; Pausch, Gold, Skelly, & Thiel, 1994), with more rapid acceleration in the last 10 years. A recent metareview (Carter, Downs, Nansen, Harrop, & Gibbs, 2014) analyzed game- and play-related content at CHI between 2003 and 2013, finding that the overall percentage of the CHI proceedings related to play and games rose from 2.5% to a peak of 9.5% in 2012. In the last few years, venues for game-related HCI work have expanded as well. From 2011 to 2013, two of this special issue’s editors (Bernhaupt & Isbister, 2013) formed a Games and Entertainment Special Community devoted to game-related HCI research at CHI, leading to the permanent addition to CHI venues of a Student Game Design Competition. And in 2014, a new ACM-sponsored conference was created as a specialized peer-reviewed venue for the intersection of HCI and Games—CHI-Play. Game-related research is clearly a valued, integral, and growing segment of HCI research

Stacking Gravitational Wave Signals from Soft Gamma Repeater Bursts

Soft gamma repeaters (SGRs) have unique properties that make them intriguing targets for gravitational wave (GW) searches. They are nearby, their burst emission mechanism may involve neutron star crust fractures and excitation of quasi-normal modes, and they burst repeatedly and sometimes spectacularly. A recent LIGO search for transient GW from these sources placed upper limits on a set of almost 200 individual SGR bursts. These limits were within the theoretically predicted range of some models. We present a new search strategy which builds upon the method used there by "stacking" potential GW signals from multiple SGR bursts. We assume that variation in the time difference between burst electromagnetic emission and burst GW emission is small relative to the GW signal duration, and we time-align GW excess power time-frequency tilings containing individual burst triggers to their corresponding electromagnetic emissions. Using Monte Carlo simulations, we confirm that gains in GW energy sensitivity of N^{1/2} are possible, where N is the number of stacked SGR bursts. Estimated sensitivities for a mock search for gravitational waves from the 2006 March 29 storm from SGR 1900+14 are also presented, for two GW emission models, "fluence-weighted" and "flat" (unweighted).Comment: 17 pages, 16 figures, submitted to PR

Wall material selection for microencapsulating glicine max and punica granatum oils by spray drying.

Pomegranate seed oil, even though highly sensitive to oxidation, has been extensively studied due to its anti-inflammatory potential. Microencapsulation by spray drying can protect the oil and increase its shelf life. Modified starch, maltodextrin and gum arabic are evaluated by a mixture design to get the better wall material composition. The better response for drying process yield (41%) and oxidative stability (42%) were achieved using modified starch and gum arabic at the same proportion as wall material

Role of cell wall on tomato fruit susceptibility to calcium deficiency disorder

The objectives of this study were to evaluate the effect of Ca2+ binding to the cell wall 14 on fruit susceptibility to BER

Blending of pressed vegetable oils from pomegranate seeds and soybean to increase functional lipids consume.

Made available in DSpace on 2018-06-07T01:05:40Z (GMT). No. of bitstreams: 1 JAPLR0700237ArtigoBlendingromaandsoyaoil.pdf: 601179 bytes, checksum: 7a071e856fe89acdcfb58ce9db64f86a (MD5) Previous issue date: 2018-05-28bitstream/item/177948/1/JAPLR-07-00237-Artigo-Blending-roma-and-soya-oil.pd

A comparison study of regional atmospheric simulations with an elastic backscattering Lidar and sunphotometry in an urban area

We describe a comparison study of Aerosol Optical Thickness (AOT) from numerical simulations using a regional atmospheric model with an elastic backscattering lidar operating at 532 nm and a sunphotometer belonging to the AERONET network at São Paulo (23° S 46° W) city, Brazil, a very populated urban area. The atmospheric model includes an aerosol emission, transport and deposition module coupled to a radiative transfer parameterization, which takes the interaction between aerosol particles and short and long wave radiation into account. A period of one week was taken as case study during the dry season (late August) when intense biomass burning activities occur at remote areas in South America, and meteorological conditions disfavor the pollution dispersion in the city of São Paulo. The situation presented here showed how smoke from biomass burning in remote areas is transported to the south-east part of Brazil and affects the optical atmospheric conditions in São Paulo. The numerical simulations are corroborated by in situ measurements of AOT obtained by lidar and sun photometry

Evidences for Tsallis non-extensivity on CMR manganites

We found, from the analysis of $M$ vs. $T$ curves of some manganese oxides (manganites), that these systems do not follow the traditional Maxwell-Boltzmann statistics, but the Tsallis statistics, within the \QTR{em}{normalized} formalism. Curves were calculated within the mean field approximation, for various ferromagnetic samples and the results were compared to measurements of our own and to various other authors published data, chosen at random from the literature. The agreement between the experimental data and calculated $M_{q}$ vs. $T^{\ast}$ curve, where $T^{\ast}$ is an effective temperature, is excellent for all the compounds. The entropic parameter, $q$, correlates in a simple way with the experimental value of $T_{c}$, irrespect the chemical composition of the compounds, heat treatment or other details on sample preparation. Examples include $q<1$ (superextensivity), $q=1$ (extensivity) and $q>1$ (subextensivity) cases.Comment: 12 pages, 3 figure