Study of the Top-quark Pair Production in Association with a Bottom-quark Pair from Fast Simulations at the LHC

A large number of top quarks will be produced at the Large Hadron Collider (LHC) for Run II period. This will allow us to measure the rare processes from the top sector in great details. We present the study of the top-quark pair production in association with a bottom-quark pair (ttbb) from fast simulations for the Compact Muon Solenoid (CMS) experiment. The differential distributions of ttbb are compared with the top-quark pair production with two additional jets (ttjj) and with the production in association with the Higgs (ttH), where the Higgs decays to a bottom-quark pair. The significances of ttbb process in the dileptonic and semileptonic decay mode are calculated with the data corresponding to an integrated luminosity of 10 fb-1, which is foreseen to be collected in the early Run II period. This study will be an important input in searching for new physics beyond the standard model as well as in searching for ttH process where the Yukawa coupling with the top quark can be directly measured.Comment: 12 pages, 12 figure

Advancement of Erosion Testing, Modeling, and Design of Concrete Pavement Subbase Layers

Concrete pavement systems have great capacity to provide long service lives; however, if the subbase layer is improperly designed or mismanaged, service life would be diminished significantly since the subbase layer performs many important roles in a concrete pavement system. The erosion of material beneath a concrete slab is an important performance-related factor that if applied to the selection of base materials can enhance the overall design process for concrete pavement systems. However, erosion of the subbase has not been included explicitly in analysis and design procedures since there is not a well accepted laboratory test and related erosion model suitable for design. Previous erosion test methods and erosion models are evaluated in terms of their utility to characterize subbase materials for erosion resistance. With this information, a new test configuration was devised that uses a Hamburg wheel-tracking device for evaluating erodibility with respect to the degree of stabilization and base type. Test devices, procedures, and results are explained and summarized for application in mechanistic design processes. A proposed erosion model is calibrated by comparing erosion to lab test results and LTPP field performance data. Subbase design guidelines are provided with a decision flowchart and a design assistant spread sheet for the economical and sustainable design of concrete pavement subbase layers by considering many design factors that affect the performance of the subbase

Chronic Treatment with Squid Phosphatidylserine Activates Glucose Uptake and Ameliorates TMT-Induced Cognitive Deficit in Rats via Activation of Cholinergic Systems

The present study examined the effects of squid phosphatidylserine (Squid-PS) on the learning and memory function and the neural activity in rats with TMT-induced memory deficits. The rats were administered saline or squid derived Squid-PS (Squid-PS 50 mg kg−1, p.o.) daily for 21 days. The cognitive improving efficacy of Squid-PS on the amnesic rats, which was induced by TMT, was investigated by assessing the passive avoidance task and by performing choline acetyltransferase (ChAT) and acetylcholinesterase (AchE) immunohistochemistry. 18F-Fluorodeoxyglucose and performed a positron emission tomography (PET) scan was also performed. In the passive avoidance test, the control group which were injected with TMT showed a markedly lower latency time than the non-treated normal group (P < 0.05). However, treatment of Squid-PS significantly recovered the impairment of memory compared to the control group (P < 0.05). Consistent with the behavioral data, Squid-PS significantly alleviated the loss of ChAT immunoreactive neurons in the hippocampal CA3 compared to that of the control group (P < 0.01). Also, Squid-PS significantly increased the AchE positive neurons in the hippocampal CA1 and CA3. In the PET analysis, Squid-PS treatment increased the glucose uptake more than twofold in the frontal lobe and the hippocampus (P < 0.05, resp.). These results suggest that Squid-PS may be useful for improving the cognitive function via regulation of cholinergic enzyme activity and neural activity

Sepsis-Like Systemic Inflammation Induced by Nano-Sized Extracellular Vesicles From Feces

Nano-sized extracellular vesicles (EVs), including exosomes, microvesicles, and other types of vesicles, are released by most mammalian cells and bacteria. We here ask whether feces contain EVs of mammalian and/or bacterial origin, and whether these EVs induce systemic inflammation. Fecal extracellular vesicles (fEVs) were isolated from mice and humans. The presence of EVs from Gram-negative and Gram-positive bacteria was detected by enzyme-linked immunosorbent assay using anti-lipid A and anti-lipoteichoic acid antibodies, whereas Western blot using anti-beta-actin antibody was employed to detect host-derived EVs in the fEVs. Further, fEVs were administered into mice by intraperitoneal injection, and inflammatory responses were investigated in the peritoneum, blood, and lungs. The role of TLR2 and TLR4 were studied using knockout mice. Significant quantities of EVs were present in feces from mice as well as humans, and derived from Gram-negative and Gram-positive bacteria, as well as the host. Bacteria-free fEVs introduced into the peritoneum induced local and systemic inflammation (including in the lungs), but fEVs from germ-free animals had weaker effects. This pronounced local and systemic inflammatory responses seemed to be induced by EVs from both Gram-negative and Gram-positive bacteria, and was attenuated in mice lacking TLR2 or TLR4. Our findings show that fEVs cause sepsis-like systemic inflammation, when introduced intraperitoneally, a process regulated by TLR2 and TLR4.11Ysciescopu

Soluble Epoxide Hydrolase Activity Determines the Severity of Ischemia-Reperfusion Injury in Kidney

Soluble epoxide hydrolase (sEH) in endothelial cells determines the plasma concentrations of epoxyeicosatrienoic acids (EETs), which may act as vasoactive agents to control vascular tone. We hypothesized that the regulation of sEH activity may have a therapeutic value in preventing acute kidney injury by controlling the concentration of EETs. In this study, we therefore induced ischemia-reperfusion injury (IRI) in C57BL/6 mice and controlled sEH activity by intraperitoneal administration of the sEH inhibitor 12-(3-adamantan-1-ylureido)-dodecanoic acid (AUDA). The deterioration of kidney function induced by IRI was partially moderated and prevented by AUDA treatment. In addition, AUDA treatment significantly attenuated tubular necrosis induced by IRI. Ischemic injury induced the down-regulation of sEH, and AUDA administration had no effect on the expression pattern of sEH induced by IRI. In vivo sEH activity was assessed by measuring the substrate epoxyoctadecenoic acid (EpOME) and its metabolite dihydroxyoctadec-12-enoic acid (DHOME). Ischemic injury had no effects on the plasma concentrations of EpOME and DHOME, but inhibition of sEH by AUDA significantly increased plasma EpOME and the EpOME/DHOME ratio. The protective effect of the sEH inhibitor was achieved by suppression of proinflammatory cytokines and up-regulation of regulatory cytokines. AUDA treatment prevented the intrarenal infiltration of inflammatory cells, but promoted endothelial cell migration and neovascularization. The results of this study suggest that treatment with sEH inhibitors can reduce acute kidney injury