ER Stress and Iron Homeostasis: A New Frontier for the UPR

The C282Y mutation of HFE accounts for the majority of cases of the iron overload disease Hereditary Hemochromatosis (HH). The conformational changes introduced by this mutation impair the HFE association with β2-microglobulin (β2m) and the cell surface expression of the protein: with two major consequences. From a functional perspective, the ability of HFE to bind to transferrin receptors 1 and 2 is lost in the C282Y mutant, thus affecting hepcidin regulation. Also due to the faulty assembly with β2m, HFE-C282Y molecules remain in the endoplasmic reticulum (ER) as aggregates that undergo proteasomal degradation and activate an Unfolded Protein Response (UPR). UPR activation, regardless of the ER stress stimuli, was shown to reshape the expression profile of iron-related genes and to decrease MHC-I cell surface expression. The possibility of a HFE-C282Y-mediated interplay between the UPR and iron homeostasis influencing disease progression and the clinical heterogeneity among C282Y carriers is discussed. The responsiveness of the ER chaperone calreticulin to both ER and iron-induced oxidative stresses, and its correlation with HH patients' phenotype, reinforce the interest of dissecting the UPR signaling/iron metabolism crosstalk and points to the potential clinical value of use of pharmacological chaperones in HFE-HH

Modelling of thermoelectric generator with heat pipe assist for range extender application

Recent trends towards electrification of vehicles favour the adoption of waste energy recovery into electricity. Battery-only Electric Vehicles (BEV) need a very large energy storage system so the use of a Range Extender (RE) may allow a significant downsizing of these bulky components. The Internal Combustion Engines (ICE) have two major discarded energy fluxes, engine cooling and exhaust gas. In Extended Range Electric Vehicles (EREV) and hybrids the potential for heat conversion into electricity is particularly convenient. The direct conversion of thermal energy into electricity, using Thermoelectric Generators (TEG) is very attractive in terms of complexity. However, current commercial TEG modules based on Seebeck effect are temperature limited, so they are unable to be in direct contact with the exhaust gases. A way to downgrade the temperature levels without reducing its potential is to interpose Heat Pipes (HP) between the exhaust gas and the modules. This control of maximum temperature at the modules is achieved by regulating the pressure of phase change of the HP fluid. Such design is convenient for engines with large thermal load variations, such as the RE being developed by the team, with a low (15kW) and a high (40kW) power mode of operation. This system will be able to operate efficiently in both modes. The present work presents the thermal modelling of such a system in order to assess the suitability of this approach. This work is complemented with the experimental work being carried out by the team in this subject, already with some published results. The model was validated with experimental data with a good correlation. Therefore, it was possible to demonstrate the potential of this system for wasted heat recovery.MIT Portugal, Fundação para a Ciência e a Tecnologia (FCT

Temperature controlled exhaust heat thermoelectric generation

The amount of energy wasted through the exhaust of an Internal Combustion Engine (ICE) vehicle is roughly the same as the mechanical power output of the engine. The high temperature of these gases (up to 1000°C) makes them intrinsically apt for energy recovery. The gains in efficiency for the vehicle could be relevant, even if a small percentage of this waste energy could be regenerated into electric power and used to charge the battery pack of a Hybrid or Extended Range Electric Vehicle, or prevent the actuation of a conventional vehicle's alternator.SFRH / BPD / 51048 / 2010MIT-Pt/EDAM-SMS/0030/200

A mechanical analysis of polydicyclopentadiene with metal inserts through flexural load

Polydicyclopentadiene (PDCPD) is being used in several products with particular success regarding to weight reduction and surface finish improvement. Despite the enough good mechanical properties presented by this thermosetting polymer, it needs to be adequately reinforced in applications requiring extra structural stability. This work studies the improvement of mechanical properties that may be achieved in PDCPD components reinforced with over-moulded steel wires by using the reaction injection moulding (RIM). Specimens, with and without metal inserts, were produced by using a special dedicated developed prototype RIM mould to be submitted to three point bending test, according to EN ISO 178:2003 standard. This paper presents and discusses the results obtained from the flexural tests, which allow concluding that the solution could be industrially used in feasible and advantageous conditions.Fundação para a Ciência e a Tecnologia (FCT)Fundos FEDER, Programa Operacional para Fatores de Competitividade – COMPETE, projeto 1384

Influence of heat pipe operating temperature on exhaust heat thermoelectric generation

Increasingly stringent targets on energy efficiency and emissions, as well as growing vehicle electrification are making attractive the electric recovery of the energy normally wasted through the tailpipe of Internal Combustion Engines. Recent developments in thermoelectrics (TE) may soon make them a viable solution for such applications. This team has been exploring the potential of using TE modules in combination with variable conductance heat pipes for transferring the exhaust heat to the generator with very low thermal resistance and at a constant, prescribed temperature. This passive temperature control eliminates the need for by-pass systems in the event of temperature overshoots. The operating temperature of a generator should be as high as possible in order to maximize the Seebeck effect. However, currently available modules are temperature limited. Moreover, the higher the HP temperature the less the usable thermal power at the exhaust will be (heat can only be transferred to from a hotter to a colder body). The present work assesses both theoretically and experimentally the influence of the HP temperature in the electric output of a thermoelectric generator. A small diesel engine and a generator were tested and it was found that a high HP operating temperature is only limitative for performance in the cases where low exhaust temperature and low engine power are present. In those cases it is possible to estimate an optimal HP temperature in order to maximize power output. The combined use of Seebeck modules and heat pipes was found to be highly advantageous in various ways.Fundação para a Ciência e a Tecnologia (FCT), FEDER e POFC CompeteMIT Portugal EDAM, Project MOBI-MP

KrP laser CVD of chromium oxide by photodissociation of Cr(CO)(6)

This work reports on the synthesis of chromium oxide thin films prepared by photodissociation of Cr(CO)(6) in an oxidizing atmosphere, using a pulsed UV laser (KrF, lambda = 248 nm). The experimental conditions, which should enable the synthesis of CrO2, are discussed and results on the deposition of CrxOy films on Al2O3 (0001) substrates are presented

Structural and Microanalytical Studies of CrO2 Thin Films on c-Sapphire by High Resolution Electron Microscopy Methods

Chromium dioxide (CrO2) has been extensively used in the magnetic recording industry. However, it is its ferromagnetic half-metallic nature that has more recently attracted much attention, primarily for the development of spintronic devices. CrO2 is the only stoichiometric binary oxide theoretically predicted to be fully spin polarized at the Fermi level. It presents a Curie temperature of ∼ 396 K, i.e. well above room temperature, and a magnetic moment of 2 mB per formula unit. However an antiferromagnetic native insulating layer of Cr2O3 is always present on the CrO2 surface which enhances the CrO2 magnetoresistance and might be used as a barrier in magnetic tunnel junctions

Assessment of metallic element contamination in sediments from the Santos - São Vicente Estuarine System

Three approaches were applied to evaluate metal contamination in 41 sediment samples from the Santos - São Vicente Estuarine System: normalization to Al, statistical analysis and sediment quality guidelines (SQGs). The results showed increases in the concentrations of Zn, Ni, Pb, Cd, Cr and Hg, which seemed to be associated with human activities. The levels of Al, Fe and Co probably were associated with crustal material or natural weathering processes. About 45% of the samples presented concentrations exceeding TEL-ERL, levels occasionally associated with adverse biological effects. Four of these samples presented concentrations above PEL-ERM, levels frequently associated with adverse biological effects.FAPES