Potential use of treated wastewater from a cattle operation in the fertigation of organic carrots

The use of treated effluents rich in nutrients and organic matter has intensified in agricultural crops, contributing to the demand for water and fertilizers. The goal of this work was to assess the effects of fertigation with treated dairy cattle wastewater, for the cultivation of carrot (Daucus carota) when applied in four different doses, under field conditions, on nutrient accumulation, productivity, and health quality in the carrot (D. carota). Wastewater from treated cattle (WTC) was treated in a pilot treatment unit (PTU). Cultivation was carried out in two beds, and the WTC applied by drippers. Nitrogen (N) was considered the base element for the dose calculation, and a 100% N dose was equivalent to 150 kg ha−1. WTC doses of 0, 100, 200, and 300% N were evaluated. Productivity was evaluated at 70 and 120 days after sowing, in the aerial part (fresh and dry mass and accumulation of nutrients), in the main roots (fresh and dry mass, accumulation of nutrients, diameter, length, and sanitary quality), and as the total productivity of the two organs. As a result, an increase in productivity was observed for all treatments with WTC and accumulation of Ca and Mg. The roots did not present contamination; therefore, the carrots were fit for human consumption. It was concluded that the application of WTC in organic cultivation of carrots is a viable alternative means of plant fertilization, providing higher root productivity than the national average, reaching 72.6 t ha−1 for a dose of 100% N, without compromising on sanitary quality and is suitable for human and animal consumption.A utilização de efluentes tratados, ricos em nutrientes e matéria orgânica, tem se intensificado nas culturas agrícolas, contribuindo para a demanda por água e fertilizantes. O objetivo deste trabalho foi avaliar os efeitos da fertirrigação com água residuária de gado leiteiro tratada para o cultivo da cenoura (Daucus carota), quando aplicada em quatro doses diferentes, em condições de campo, no acúmulo de nutrientes, produtividade e qualidade sanitária. As águas residuárias de bovinocultura (ARB) foram tratadas em uma unidade piloto de tratamento (UPT). O cultivo foi realizado em dois canteiros, sendo a ARB aplicada por gotejadores. O nitrogênio (N) foi considerado o elemento base para o cálculo da dose, e uma dose de 100% de N foi equivalente a 150 kg ha-1. Doses da ARB de 0, 100, 200 e 300% de N foram avaliadas. A produtividade foi aferida aos 70 e 120 dias após a semeadura, na parte aérea (massa fresca e seca e acúmulo de nutrientes), nas raízes principais (massa fresca e seca, acúmulo de nutrientes, diâmetro, comprimento e qualidade sanitária) e nas duas partes (produtividade total). Como resultado, observou-se aumento na produtividade para todos os tratamentos com ARB e acúmulo de N, Ca e Mg. As raízes não apresentaram contaminação, portanto as cenouras eram próprias para consumo humano. Concluiu-se que a aplicação da ARB no cultivo orgânico de cenoura é uma alternativa viável de adubação das plantas. Proporciona produtividade de raízes superior à média nacional, chegando a 72,6 t ha-1 para uma dose de 100% N, sem comprometer a qualidade sanitária do produto, que é adequado para consumo humano e animal

PHYSICOCHEMICAL CHARACTERISTICS, ANTIOXIDANT CAPACITY AND PHENOLIC COMPOUNDS OF TOMATOES FERTIGATED WITH DIFFERENT NITROGEN RATES

The objective of this work was to evaluate the physicochemical and microbiological characteristics, antioxidant capacity and phenolic compounds of organic cherry tomatoes grown under fertigation with organic dairy cattle wastewater (DCW) with different nitrogen rates. Tomato plants, grown in an agroecological farm in Seropédica, State of Rio de Janeiro, Brazil, were subjected to four different nitrogen rates (T1=0, T2=50, T3=100 and T4=150% of N). The moisture, lipids, ashes, protein and total fiber contents, soluble solids (ºBrix), reducing and total sugars (%), pH and total titratable acidity (mg NaOH per 100 g) were evaluated. The total phenolic content (TPC) and the antioxidant capacity was determined by the DPPH and FRAP methods. The different nitrogen rates (%N) affected the pH, protein and soluble solids contents. The increase in %N increased the antioxidant capacities, according to the DPPH assay, and TPC. On the other hand, the tomatoes under fertigation with the highest %N presented lower antioxidant capacities according to the FRAP assay. The fertigation did not affect the microbiological characteristics of the tomatoes, which presented fecal coliforms count <3 NMP g-1 and absence of Salmonella in 25 g

Non-invasive electromechanical assessment during atrial fibrillation identifies underlying atrial myopathy alterations with early prognostic value

Electromechanical characterization during atrial fibrillation (AF) remains a significant gap in the understanding of AF-related atrial myopathy. This study reports mechanistic insights into the electromechanical remodeling process associated with AF progression and further demonstrates its prognostic value in the clinic. In pigs, sequential electromechanical assessment during AF progression shows a progressive decrease in mechanical activity and early dissociation from its electrical counterpart. Atrial tissue samples from animals with AF reveal an abnormal increase in cardiomyocytes death and alterations in calcium handling proteins. High-throughput quantitative proteomics and immunoblotting analyses at different stages of AF progression identify downregulation of contractile proteins and progressive increase in atrial fibrosis. Moreover, advanced optical mapping techniques, applied to whole heart preparations during AF, demonstrate that AF-related remodeling decreases the frequency threshold for dissociation between transmembrane voltage signals and intracellular calcium transients compared to healthy controls. Single cell simulations of human atrial cardiomyocytes also confirm the experimental results. In patients, non-invasive assessment of the atrial electromechanical relationship further demonstrate that atrial electromechanical dissociation is an early prognostic indicator for acute and long-term rhythm control. Electromechanical characterization during atrial fibrillation (AF) remains a significant gap in the understanding of AF-related atrial myopathy. Here, the authors use non-invasive atrial electromechanical assessment during AF to identify early remodeling changes associated with underlying myopathy, which in the clinic decrease the probability of acute and mid-term successful rhythm control

Non-invasive electromechanical assessment during atrial fibrillation identifies underlying atrial myopathy alterations with early prognostic value.

Electromechanical characterization during atrial fibrillation (AF) remains a significant gap in the understanding of AF-related atrial myopathy. This study reports mechanistic insights into the electromechanical remodeling process associated with AF progression and further demonstrates its prognostic value in the clinic. In pigs, sequential electromechanical assessment during AF progression shows a progressive decrease in mechanical activity and early dissociation from its electrical counterpart. Atrial tissue samples from animals with AF reveal an abnormal increase in cardiomyocytes death and alterations in calcium handling proteins. High-throughput quantitative proteomics and immunoblotting analyses at different stages of AF progression identify downregulation of contractile proteins and progressive increase in atrial fibrosis. Moreover, advanced optical mapping techniques, applied to whole heart preparations during AF, demonstrate that AF-related remodeling decreases the frequency threshold for dissociation between transmembrane voltage signals and intracellular calcium transients compared to healthy controls. Single cell simulations of human atrial cardiomyocytes also confirm the experimental results. In patients, non-invasive assessment of the atrial electromechanical relationship further demonstrate that atrial electromechanical dissociation is an early prognostic indicator for acute and long-term rhythm control.This work was supported by the European Union Horizon 2020 research and innovation program under Grant Agreement#965286. The study was also supported by the Ministry of Science and Innovation (MCIN) (PID2019- 109329RB-I00 and PGC2018-097019-B-I00) funded by MCIN / AEI / 10.13039/501100011033, the Instituto de Salud Carlos III (Fondo de Investigación Sanitaria grant PRB3) (PT17/0019/0003- ISCIII-SGEFI / ERDF, ProteoRed), the Fundación Interhospitalaria para la Investigación Cardiovascular, the Fundación Salud 2000 and by “la Caixa” Banking Foundation (project code HR17-00247). The Centro Nacional de Investigaciones Cardiovasculares (CNIC) is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (CEX2020- 001041-S funded by MICIN/AEI/10.13039/501100011033). We also thank Asunción Conde and Sergey Mironov for their support on monitoring database quality and advice in ECG signal processing, respectively.S

A queda da Alianza: neoliberalismo, conflito social e crise política na Argentina

El artículo aborda la crisis política de 1999-2001 en Argentina, bajo el Gobierno de la Alianza, que sucedió al Partido Justicialista tras la introducción de las reformas neoliberales en los noventa. Analizamos la continuidad de las políticas neoliberales de gestión de la crisis, el ascenso de la conflictividad social y la supervivencia de prácticas de corrupción, que llevaron a la Alianza a un inédito proceso de desintegración interna y deslegitimación social. La crisis política se agravó con el fuerte crecimiento del voto negativo y la derrota de la Alianza en las elecciones legislativas de octubre de 2001, y finalizó con una rebelión popular que derribó al Gobierno en diciembre, en una situación de depresión económica y bancarrota financiera.This article addresses the political crisis in Argentina in 1999-2001, under the government of the Alianza which succeeded the Justicialist Party following the introduction of neoliberal reforms in the nineties. We analyze the continuity of neoliberal policies of crisis management, the rise in social conflict and the survival of corrupt practices that led the Alianza towards an unprecedented process of internal disintegration and social delegitimization. The political crisis was aggravated by the large increase in negative voting and the Alianza’s defeat in the legislative elections of October 2001, and ended with a popular uprising that toppled the government in December, in a context of economic depression and financial bankruptcy.Este artigo aborda a crise política de 1999-2001 na Argentina, sob o Governo da Alianza, que sucedeu o Partido Justicialista depois da introdução das reformas neoliberais nos noventa. Analisamos a continuidade das políticas neoliberais de gestão da crise, a ascensão do conflito social e a sobrevivência de práticas de corrupção que levaram a Alianza a um inédito processo de desintegração interna e deslegitimação social. A crise política se agravou com o forte crescimento do voto negativo e da derrota da Alianza nas eleições legislativas de outubro de 2001 e finalizou com uma rebelião popular que derrubou o Governo em dezembro, em uma situação de depressão econômica e bancarrota financeira.Fil: Salvia, Sebastian Pedro. Universidad Nacional de Quilmes. Centro de Investigaciones sobre Economía y Sociedad en la Argentina Contemporánea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Magnesium supplementation alleviates drought damage during vegetative stage of soybean plants.

Our working hypothesis was that magnesium (Mg) supplementation modulates plant performance under low water availability and improves drought tolerance in soybean genotypes. Plants of Bônus 8579, M8808 and TMG1180 genotypes were grown under field conditions and subjected to three water stress treatments (control, moderate and severe stress) and three Mg levels [0.9 (low), 1.3 (adequate) and 1.7 cmolc dm-³ (supplementation)]. After 28 days of drought imposition, the growth parameters, osmotic potential, relative water content, leaf succulence, Mg content and photosynthetic pigments were assessed. In general, drought drastically decreased the growth in all genotypes, and the reductions were intensified from moderate to severe stress. Under adequate Mg supply, TMG1180 was the most drought-tolerant genotype among the soybean plants, but Mg supplementation did not improve its tolerance. Conversely, although the M8808 genotype displayed inexpressive responses to drought under adequate Mg, the Mg-supplemented plants were found to have surprisingly better growth performance under stress compared to Bônus 8579 and TMG1180, irrespective of drought regime. The improved growth of high Mg-treated M8808-stressed plants correlated with low osmotic potential and increased relative water content, as well as shoot Mg accumulation, resulting in increased photosynthetic pigments and culminating in the highest drought tolerance. The results clearly indicate that Mg supplementation is a potential tool for alleviating water stress in M8808 soybean plants. Our findings suggest that the enhanced Mg-induced plant acclimation resulted from increased water content in plant tissues and strategic regulation of Mg content and photosynthetic pigments