Frontal Asymmetry Changes Following Passive Hypo-Hydration

INTRODUCTION: Universal fluid consumption prescriptions among both athletic and non-athletic populations continue to elicit both hypo- and hyper-hydration (hypo-natremia). Thus, the need to find novel, identifiable traits within individuals who may be at higher risk to experience hypo-hydration, as well as hypo-natremia, is warranted. Of interest here, behavior is encapsulated by two motivational systems, referred to as approach/reward (R) or avoidance/non-reward (NR), respectively, in response to a behavioral stimulus. Past literature has revealed individual differences in consumptive traits of various types of foods between these motivational systems. Therefore, the purpose of this study was to evaluate changes in R/NR via frontal asymmetry (FA) in response to water exposure during eu-hydrated and hypo-hydrated conditions. METHODS: Participants included 11 active college-aged males. Electroencephalogram (EEG) assessed motivational orientations to water exposure in both eu-hydrated and hypo-hydrated states. A 5-minute nature video was used to stabilize mood, followed by 2 minutes of resting EEG recordings. Then, FA was assessed with the presentation of a glass of ice water, with instructions to observe, but not consume for 3 minutes. FA was re-assessed in the same manner following a dehydration trial (~90 minutes). Dehydration trials were performed in a controlled hot water bath at a mean of 39°C. Participants remained in the bath until 2% body mass loss, with no fluids consumed during the trials. Urine voids were collected before and after trials to assess specific gravity and color. Body core temperature was monitored throughout the protocol, with \u3e38.7°C set as criteria for removal of the subject. Participants reported perceptual affective measures using Feeling Scale (FS) and Felt Arousal Scale (FAS) throughout the session to account for any unusual affective states. RESULTS: EEG recordings were applied to the Frontal Asymmetry Index (FAI) (log right alpha power minus log left alpha power). Higher scores on the FAI indicate higher relative left frontal lobe activity, corresponding with an affinity to the water (R). Where as a lower score indicates higher relative right frontal activity, thus less reward motivation (NR). Data revealed that FAI decreased by a mean of 0.055 ±0.193 from eu-hydrated to hypo-hydrated states, with eu-hydration and hypo-hydration FAI of 0.06 and 0.004 respectively. In total, 37.4% of participants showed an increased affinity(R) for water from eu-hydrated to hypo-hydrated states. Pre and post FS were 2.91 ±1.51 and .45 ±2.88, respectively. Pre and post FAS were 2.73 ±.91 and 2.73 ±1.49. DISCUSSION: Reward/non-reward motivational orientations (affinity to water) changes were less than anticipated following hypo-hydration trials. However, data revealed that 37.4% of participants exhibited an increased affinity for water according to the FAI scale, supporting the hypothesis. It is possible that 2% body mass loss is insufficient to create distinct motivational orientation changes within the present sample. Due to the small sample size of the current study, little can be concluded thus far. Additional research addressing the aforementioned methods, as well as larger sample sizes is warranted. If motivational orientation state variability can be established during eu-hydrated and hypo-hydrated states, it may be possible to identify the consumptive tendencies of fluids among individuals

Linezolid-Associated Thrombocytopenia in Children with Renal Impairment

Poster presented at ID Week, October 2013, San Francisco, California

Misoprostol Inhibits Lipopolysaccharide-Induced Pro-inflammatory Cytokine Production by Equine Leukocytes

Pro-inflammatory cytokines including tumor necrosis factor α (TNFα), IL-1β, IL-6, and IL-8 are potent immune mediators that exacerbate multiple equine diseases such as sepsis and laminitis. Unfortunately, safe and effective cytokine-targeting therapies are lacking in horses; therefore, novel mechanisms of inhibiting cytokine production are critically needed. One potential mechanism for inhibiting cytokine synthesis is elevation of intracellular cyclic AMP (cAMP). In human leukocytes, intracellular cAMP production is induced by activation of E-prostanoid (EP) receptors 2 and 4. These receptors can be targeted by the EP2/4 agonist and prostaglandin E1 analog, misoprostol. Misoprostol is currently used as a gastroprotectant in horses but has not been evaluated as a cytokine-targeting therapeutic. Thus, we hypothesized that misoprostol treatment would inhibit pro-inflammatory cytokine production by lipopolysaccharide (LPS)-stimulated equine leukocytes in an in vitro inflammation model. To test this hypothesis, equine leukocyte-rich plasma (LRP) was collected from 12 healthy adult horses and used to model LPS-mediated inflammatory signaling. LRP was treated with varying concentrations of misoprostol either before (pretreated) or following (posttreated) LPS stimulation. LRP supernatants were assayed for 23 cytokines using an equine-specific multiplex bead immunoassay. Leukocytes were isolated from LRP, and leukocyte mRNA levels of four important cytokines were evaluated via RT-PCR. Statistical differences between treatments were determined using one-way RM ANOVA (Holm–Sidak post hoc testing) or Friedman’s RM ANOVA on Ranks (SNK post hoc testing), where appropriate (p < 0.05, n = 3–6 horses). These studies revealed that misoprostol pre- and posttreatment inhibited LPS-induced TNFα and IL-6 protein production in equine leukocytes but had no effect on IL-8 protein. Interestingly, misoprostol pretreatment enhanced IL-1β protein synthesis following 6 h of LPS stimulation, while misoprostol posttreatment inhibited IL-1β protein production after 24 h of LPS stimulation. At the mRNA level, misoprostol pre- and posttreatment inhibited LPS-induced TNFα, IL-1β, and IL-6 mRNA production but did not affect IL-8 mRNA. These results indicate that misoprostol exerts anti-inflammatory effects on equine leukocytes when applied before or after a pro-inflammatory stimulus. However, the effects we observed were cytokine-specific and sometimes differed at the mRNA and protein levels. Further studies are warranted to establish the inhibitory effects of misoprostol on equine cytokine production in vivo

Development and validation of a new MRI simulation technique that can reliably estimate optimal in vivo scanning parameters in a glioblastoma murine model

BACKGROUND: Magnetic Resonance Imaging (MRI) relies on optimal scanning parameters to achieve maximal signal-to-noise ratio (SNR) and high contrast-to-noise ratio (CNR) between tissues resulting in high quality images. The optimization of such parameters is often laborious, time consuming, and user-dependent, making harmonization of imaging parameters a difficult task. In this report, we aim to develop and validate a computer simulation technique that can reliably provide optimal in vivo scanning parameters ready to be used for in vivo evaluation of disease models. METHODS: A glioblastoma murine model was investigated using several MRI imaging methods. Such MRI methods underwent a simulated and an in vivo scanning parameter optimization in pre- and post-contrast conditions that involved the investigation of tumor, brain parenchyma and cerebrospinal fluid (CSF) CNR values in addition to the time relaxation values of the related tissues. The CNR tissues information were analyzed and the derived scanning parameters compared in order to validate the simulated methodology as a reliable technique for optimal in vivo scanning parameters estimation. RESULTS: The CNRs and the related scanning parameters were better correlated when spin-echo-based sequences were used rather than the gradient-echo-based sequences due to augmented inhomogeneity artifacts affecting the latter methods. Optimal in vivo scanning parameters were generated successfully by the simulations after initial scanning parameter adjustments that conformed to some of the parameters derived from the in vivo experiment. CONCLUSION: Scanning parameter optimization using the computer simulation was shown to be a valid surrogate to the in vivo approach in a glioblastoma murine model yielding in a better delineation and differentiation of the tumor from the contralateral hemisphere. In addition to drastically reducing the time invested in choosing optimal scanning parameters when compared to an in vivo approach, this simulation program could also be used to harmonize MRI acquisition parameters across scanners from different vendors

Maternal Restricted- and Over-Feeding During Gestation Result in Distinct Lipid and Amino Acid Metabolite Profiles in the Longissimus Muscle of the Offspring

Maternal over- and restricted-feeding during gestation have similar negative consequences for the offspring, including decreased muscularity, increased adiposity, and altered metabolism. Our objective was to determine the effects of poor maternal nutrition during gestation (over- and restricted-feeding) on the offspring muscle metabolite profile. Pregnant ewes (n = 47) were fed 60% (RES), 100% (CON), or 140% (OVER) of NRC requirements starting at day 30.2 ± 0.2 of gestation. Offspring sample collection occurred at days 90 and 135 of gestation, and within 24 h of birth. C2C12 myoblasts were cultured in serum collected from offspring at birth (n = 18; 6 offspring per treatment) for analysis of oxidative and glycolytic capacity. Unbiased metabolite analysis of longissimus muscle samples (n = 72; 8 fetuses per treatment per time point) was performed using mass spectrometry. Data were analyzed by ANOVA for main effects of treatment, time point, and their interaction. Cells cultured in serum from RES offspring exhibited increased proton leak 49% (p = 0.01) compared with CON, but no other variables of mitochondrial respiration or glycolytic function were altered. Mass spectrometry identified 612 metabolites. Principle component analysis identified day of gestation as the primary driver of metabolic change; however, maternal diet also altered the lipid and amino acid profiles in offspring. The abundance of 53 amino acid metabolites and 89 lipid metabolites was altered in RES compared with CON (p ≤ 0.05), including phospholipids, sphingolipids, and ceramides within the lipid metabolism pathway and metabolites involved in glutamate, histidine, and glutathione metabolism. Similarly, abundance of 63 amino acid metabolites and 70 lipid metabolites was altered in OVER compared with CON (p ≤ 0.05). These include metabolites involved in glutamate, histidine, lysine, and tryptophan metabolism and phosphatidylethanolamine, lysophospholipids, and fatty acids involved in lipid metabolism. Further, the amino acid and lipid profiles diverged between RES and OVER, with 69 amino acid and 118 lipid metabolites differing (p ≤ 0.05) between groups. Therefore, maternal diet affects metabolite abundance in offspring longissimus muscle, specifically metabolites involved in lipid and amino metabolism. These changes may impact post-natal skeletal muscle metabolism, possibly altering energy efficiency and long-term health

Emergency Preparedness: An Analysis of Policy Leader and Community Perspectives

This report, which was produced for then-Congressman Bob Riley's office, provided findings regarding the state of emergency preparedness in Calhoun County, Alabama, related to chemical weapons (CW) storage and incineration at the Anniston Army Depot. The analysis addressed the following research question. Given that CW incineration is set to start at the Anniston Army Depot in September 2002, what information would provide the basis for practical dialogue about emergency preparedness in Calhoun County and provide a possible foundation for policy leaders to reach consensus over this critical issue in order to ensure citizen acceptance, understanding, and compliance

Persistent net release of carbon dioxide and methane from an Alaskan lowland boreal peatland complex

Permafrost degradation in peatlands is altering vegetation and soil properties and impacting net carbon storage. We studied four adjacent sites in Alaska with varied permafrost regimes, including a black spruce forest on a peat plateau with permafrost, two collapse scar bogs of different ages formed following thermokarst, and a rich fen without permafrost. Measurements included year-round eddy covariance estimates of net carbon dioxide (CO2), mid-April to October methane (CH4) emissions, and environmental variables. From 2011 to 2022, annual rainfall was above the historical average, snow water equivalent increased, and snow-season duration shortened due to later snow return. Seasonally thawed active layer depths also increased. During this period, all ecosystems acted as slight annual sources of CO2 (13–59 g C m−2 year−1) and stronger sources of CH4 (11–14 g CH4 m−2 from ~April to October). The interannual variability of net ecosystem exchange was high, approximately ±100 g C m−2 year−1, or twice what has been previously reported across other boreal sites. Net CO2 release was positively related to increased summer rainfall and winter snow water equivalent and later snow return. Controls over CH4 emissions were related to increased soil moisture and inundation status. The dominant emitter of carbon was the rich fen, which, in addition to being a source of CO2, was also the largest CH4 emitter. These results suggest that the future carbon-source strength of boreal lowlands in Interior Alaska may be determined by the area occupied by minerotrophic fens, which are expected to become more abundant as permafrost thaw increases hydrologic connectivity. Since our measurements occur within close proximity of each other (≤1 km2), this study also has implications for the spatial scale and data used in benchmarking carbon cycle models and emphasizes the necessity of long-term measurements to identify carbon cycle process changes in a warming climate

The Grizzly, January 30, 1987

U.C. Master Plan Features Art, Roads, Humanities Building • Aggressive Couple Sparks Conflicts and Challenges • Art Critic to Explain Sculpture • Letter: Abortion Issue\u27s Indefinite Life • Editorial: Silent Scream Meets U.C. Yawn • News Notes: Effectiveness Training Course Offered; Stress Management Workshops; Who\u27s Who at Ursinus?; Voice Workshop Offered; Enhance Self Presentation; Grammar and Writing Course • Jones Recalls Fetterolf of Yesteryear • Racich has Bears Rolling Toward MAC Riches • Bear Hoopsters Fall From First • Men Mers Rip Scranton in First Historical Meeting • Men\u27s Track Banking on Distance and Field • Gymnasts Open Semester With Split • Soccer\u27s Quinn a Standout • Swimmin\u27 Women Use Off-Season Workouts to Advantage at 3-3 • Odgers Makes Grade on and off Field • Davidson Assumes Presidency of PSAHPERD • Arthur Vining Davis Foundation Offers $60,000 to Support Residential Village • Development\u27s Randolph Introduces STAR Committee • Alderfer Takes Championship, Badminton Opens • Gall Finds the Way it is Fresh and New • Lionarons Leads Lecture • 1987 Class Chairmen Sought by Applicationhttps://digitalcommons.ursinus.edu/grizzlynews/1179/thumbnail.jp

Alterations of immune response of non-small lung cancer with azacytidine

Innovative therapies are needed for advanced Non-Small Cell Lung Cancer (NSCLC). We have undertaken a genomics based, hypothesis driving, approach to query an emerging potential that epigenetic therapy may sensitize to immune checkpoint therapy targeting PD-L1/PD-1 interaction. NSCLC cell lines were treated with the DNA hypomethylating agent azacytidine (AZA - Vidaza) and genes and pathways altered were mapped by genome-wide expression and DNA methylation analyses. AZA-induced pathways were analyzed in The Cancer Genome Atlas (TCGA) project by mapping the derived gene signatures in hundreds of lung adeno (LUAD) and squamous cell carcinoma (LUSC) samples. AZA up-regulates genes and pathways related to both innate and adaptive immunity and genes related to immune evasion in a several NSCLC lines. DNA hypermethylation and low expression of IRF7, an interferon transcription factor, tracks with this signature particularly in LUSC. In concert with these events, AZA up-regulates PD-L1 transcripts and protein, a key ligand-mediator of immune tolerance. Analysis of TCGA samples demonstrates that a significant proportion of primary NSCLC have low expression of AZA-induced immune genes, including PD-L1. We hypothesize that epigenetic therapy combined with blockade of immune checkpoints - in particular the PD-1/PD-L1 pathway - may augment response of NSCLC by shifting the balance between immune activation and immune inhibition, particularly in a subset of NSCLC with low expression of these pathways. Our studies define a biomarker strategy for response in a recently initiated trial to examine the potential of epigenetic therapy to sensitize patients with NSCLC to PD-1 immune checkpoint blockade