The Microenvironment-Specific Transformation of Adult Stem Cells Models Malignant Triton Tumors

Here, we demonstrated the differentiation potential of murine muscle-derived stem/progenitor cells (MDSPCs) toward myogenic, neuronal, and glial lineages. MDSPCs, following transplantation into a critical-sized sciatic nerve defect in mice, showed full regeneration with complete functional recovery of the injured peripheral nerve at 6 weeks post-implantation. However, several weeks after regeneration of the sciatic nerve, neoplastic growths were observed. The resulting tumors were malignant peripheral nerve sheath tumors (MPNSTs) with rhabdomyoblastic differentiation, expressing myogenic, neurogenic, and glial markers, common markers of human malignant triton tumors (MTTs). No signs of tumorigenesis were observed 17 weeks post-implantation of MDSPCs into the gastrocnemius muscles of dystrophic/mdx mice, or 1 year following subcutaneous or intravenous injection. While MDSPCs were not oncogenic in nature, the neoplasias were composed almost entirely of donor cells. Furthermore, cells isolated from the tumors were serially transplantable, generating tumors when reimplanted into mice. However, this transformation could be abrogated by differentiation of the cells toward the neurogenic lineage prior to implantation. These results establish that MDSPCs participated in the regeneration of the injured peripheral nerve but transformed in a microenvironment- and time-dependent manner, when they likely received concomitant neurogenic and myogenic differentiation signals. This microenvironment-specific transformation provides a useful mouse model for human MTTs and potentially some insight into the origins of this disease

Геоэкологическая характеристика и проект мониторинга на территории ОАО "Фармстандарт-Томскхимфарм" (г. Томск)

Работа включает в себя геоэкологическую характеристику объекта исследования - "ОАО Фармстандарт Томскхимфарм". Описание воздействия деятельности предприятия на окружающую среду. Составление программы производственного экологического контроля для данного предприятия. Также раздел производственной безопасности и финансового менеджмента.The work includes the geoecological characteristics of the research object - OJSC "Pharmstandard Tomskkhimpharm". Description of the impact of the enterprise on the environment. Drawing up a program of industrial environmental control for this enterprise. Also the section of industrial safety and financial management

Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice

A decline in the regenerative capacity of adult stem cells with aging is well documented. As a result of this decline, the efficacy of autologous stem cell therapies is likely to decline with increasing donor age. In these cases, strategies to restore the function of aged stem cells would have clinical utility. Globally, the transcription factor NF-κB is up-regulated in aged tissues. Given the negative role that NF-κB plays in myogenesis, we investigated whether the age-related decline in the function of muscle-derived stem/progenitor cells (MDSPCs) could be improved by inhibition of NF-κB. Herein, we demonstrate that pharmacologic or genetic inhibition of NF-κB activation increases myogenic differentiation and improves resistance to oxidative stress. Our results suggest that MDSPC “aging” may be reversible, and that pharmacologic targeting of pathways such as NF-κB may enhance the efficacy of cell-based therapies

Student Satisfaction with Hostel facilities: A Case Study of Njala University, Njala Campus, Sierra Leone

Aim and Scope: The importance of students' social environments in their day-to-day lives cannot be overstated. The extent to which students are happy in their hostel depends on its facilities' availability, adequacy, and functionality. A case study of Njala University, Njala campus, was used to determine the amenities provided and the level of satisfaction the students derived from those amenities. Methods: A cross-sectional research design was used to conduct the research through in-field observations and a standardized questionnaire. The study's sample size was estimated at 482 total students. SPSS version 26.0 was used for the data analysis. Result: The study revealed that 82.0% of the respondents were within the age group 20-29 years, and more than two-thirds (70.7%) of the respondents were living in overcrowded rooms with a population of more than the required number of persons per room; this is attributed to the squatting phenomenon among the hostel occupants with the attendant implications of increasing pressure on the available facilities. Equally, students were reasonably satisfied with hostel facilities such as recreational grounds, security status, Library/ICT, waste management and electricity. Results showed respondents were unhappy with the adequacy and performance of certain facilities such as the drainage system, foam, toilet, water supply, kitchen, room size, laundry, and firefighting device. Conclusion: For student convenience, as enrollment continues to rise, the paper suggests that public-private partnerships be established to fund the construction of other hostels with updated designs and amenities

Student Satisfaction with Hostel Facilities: A Case Study of Njala University, Njala Campus, Sierra Leone

Aim and Scope: The importance of students' social environments intheir day-to-day lives cannot be overstated. The extent to which students arehappy in their hostel depends on its facilities' availability, adequacy, andfunctionality. A case study of Njala University, Njala campus, was used todetermine the amenities provided and the level of satisfaction the students derived from those amenities. Methods: A cross-sectional research designwas used to conduct the research through a close-ended questionnaire. Thestudy's sample size was 482 students. SPSS version 26.0 was used for the dataanalysis. Result: Most (82.0%) of the respondents were within the age group20-29 years, and more than two-thirds (70.7%) were living in overcrowdedrooms with double the number than the room was designed for.; this isattributed to the common phenomenon of students who get a place sub-lettingto their less fortunate colleagues on a cost-sharing basis. Overcrowding hasimplications of increasing pressure on the available facilities. Students werereasonably satisfied with hostel facilities such as recreational grounds, securitystatus, Library/ICT, waste management, and electricity, but very dissatisfiedwith the drainage system, state of mattresses, toilet facilities, water supply,kitchen, room size, laundry, and lack of firefighting devices. Conclusion :Students are dissatisfied with current facilities as enrollment continues to risefaster than Government intervention the paper suggests that a public-privatepartnership be established to fund the construction of other hostels withupdated designs and amenities

Student Satisfaction with Hostel facilities: A Case Study of Njala University, Njala Campus, Sierra Leone

Aim and Scope: The importance of students' social environments in their day-to-day lives cannot be overstated. The extent to which students are happy in their hostel depends on its facilities' availability, adequacy, and functionality. A case study of Njala University, Njala campus, was used to determine the amenities provided and the level of satisfaction the students derived from those amenities. Methods: A cross-sectional research design was used to conduct the research through in-field observations and a standardized questionnaire. The study's sample size was estimated at 482 total students. SPSS version 26.0 was used for the data analysis. Result: The study revealed that 82.0% of the respondents were within the age group 20-29 years, and more than two-thirds (70.7%) of the respondents were living in overcrowded rooms with a population of more than the required number of persons per room; this is attributed to the squatting phenomenon among the hostel occupants with the attendant implications of increasing pressure on the available facilities. Equally, students were reasonably satisfied with hostel facilities such as recreational grounds, security status, Library/ICT, waste management and electricity. Results showed respondents were unhappy with the adequacy and performance of certain facilities such as the drainage system, foam, toilet, water supply, kitchen, room size, laundry, and firefighting device. Conclusion: For student convenience, as enrollment continues to rise, the paper suggests that public-private partnerships be established to fund the construction of other hostels with updated designs and amenities