Supercritical CO2 extraction of cumbaru oil (dipteryx alata vogel) assisted by ultrasound: global yield, kinetics and fatty acid composition

Cumbaru almond is obtained from a plant of the Brazilian Cerrado and contains elevated amount of oil. The aims of this work were to extract the cumbaru oil by supercritical CO2 extraction with and without ultrasound (US) and to evaluate the process by means of global yield, modeling extraction kinetics and fatty acids profile. An experimental design was proposed to obtain cumbaru oil by supercritical fluid extraction (SFE) without US. The process was evaluated at 40, 50 and 60 °C and 15, 25 and 35 MPa. The best conditions to recover cumbaru oil were 40 and 50 °C at 35 MPa, in which 22.6 and 22.8 g of oil/g of raw material were obtained, respectively. The fatty acid profile was not affected by variations in SFE conditions. The BET-based model and broken-intact cell model were able to fit and represent adequately the SC-CO2 extraction kinetics of cumbaru oil (AARD < 10%). The oil presented high concentration of oleic (∼49%) and linoleic (∼26%) acids. A higher initial rate of fatty acids was achieved when the extraction process was assisted by US, and US did not modify the fatty acid composition of the whole oil and the final global yield1077583CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP142373/2013-32952/20112013/02203-6; 2014/00372-

Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis

Background: This study evaluated the effect of the solvent on the extraction of antioxidant compounds from black mulberry (Morus nigra), blackberry (Rubus ulmifolius) and strawberry (Fragaria x ananassa). Different extracts of each berry were evaluated from the determination of total phenolic content, anthocyanin content and antioxidant capacity, and data were applied to the principal component analysis (PCA) to gain an overview of the effect of the solvent in extraction method.Results: For all the berries analyzed, acetone/water (70/30, v/v) solvent mixture was more efficient solvent in the extracting of phenolic compounds, and methanol/water/acetic acid (70/29.5/0.5, v/v/v) showed the best values for anthocyanin content. Mixtures of ethanol/water (50/50, v/v), acetone water/acetic acid (70/29.5/0.5, v/v/v) and acetone/water (50/50, v/v) presented the highest antioxidant capacities for black mulberries, blackberries and strawberries, respectively.Conclusion: Antioxidants extractions are extremely affected by the solvent combination used. In addition, the obtained extracts with the organic solvent-water mixtures were distinguished from the extracts obtained with pure organic solvents, through the PCA analysis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Chemical characterization of leaves of organically grown carrot Dacus carota L.) in various stages of development for use as food

In Brazil, street markets and vegetable distributors discard vegetable leaves and stems, including those of carrot (Dacus carota L.). Seeking to reduce the waste of vegetable parts, this study characterized chemically the leaves of organically grown carrot in three stages of development to determine the best time for their removal and consumption as food. The leaves were dehydrated in an oven at 70 °C for 43 hours and analyzed for chemical composition, antioxidant activity, chlorophyll content, fatty acid composition, and also calcium (Ca), sodium (Na), potassium (K), magnesium (Mg), manganese (Mn), iron (Fe), zinc (Zn), and copper (Cu) contents. The analyses indicated 100 days of development as the ideal stage for the removal and consumption of carrot leaves with good antioxidant activity requiring only 63.78 ± 0.5 mg.L-1 methanol leaf extract to inhibit 50% of the concentration of the free radical DPPH (2,2-diphenyl-1picrilidrazil), and total protein and alpha-linolenic acid (18:3 n-3/LNA) contents of 18.23% ± 2.8 and 876.55 ± 20.62 mg.100 g-1 of dry matter, respectively

Antioxidant activity and lipid oxidation in milk from cows with soybean oil and propolis extract added to their feed

Abstract The presence of PUFA (polyunsaturated fatty acids) in cow milk, including conjugated linoleic acids (CLA) isomers, makes milk susceptible to lipid oxidation, and may lead to the formation of volatile products responsible for a rancid smell. Consequently, including natural antioxidants in the diet of dairy cattle can assist in preventing lipid oxidation. In this study, soybean oil (a source of PUFA) and different ethanolic extracts of Brazilian propolis were included in the cows' feed, and the antioxidant activity and lipid oxidation of the animals' milk was assessed. Antioxidant activity was analyzed using the DPPH method, potassium ferricyanide reduction and chelating ability. Total phenolic compounds and flavonoids were also determined. Lipid oxidation was assessed by determining conjugated dienes and the production of aldehydes (propanal, pentanal, hexanal and octanal). Increased antioxidant activity was seen in the samples of milk from cows receiving propolis. Similarly, the addition of ethanolic extract of propolis to the diet of dairy cattle increased milk protection for two treatments with propolis against lipid oxidation in terms of production of propanal and hexanal. These results confirm the positive effect of applying this natural additive to the diet of lactating cows, improving the nutritional quality of the milk produced

Perfil de ácidos graxos na carne de ovelhas de descarte alimentadas com dietas contendo níveis de inclusão de linhaça, confinadas por diferentes períodos

Linseed plays an important role in animal nutrition as it contains long-chain fatty acids in its composition, which, once absorbed, are incorporated into meat and milk. For evaluate the concentration of fatty acids in the Longissimus dorsi muscle of culling ewes fed diets containing levels of inclusion of linseed, 88 culling ewes were used with an average initial body weight of 37.65 ± 6.98 kg were distributed in a completely randomized design with 12 treatments. Treatments consisted of the interaction between levels of linseed (0, 5, 10, and 15%) and days in feedlot (30, 45, and 60). The most present fatty acids in the composition of the muscle L. dorsi were palmitic (27.32 g 100 g-1) and stearic (17.77 g 100 g-1). Saturated acids remained at low levels as the animals were fed greater levels of linseed, demonstrating the importance of introducing quality foods in animal feeding. Oleic acid was the most present monounsaturated fatty acid, with 40 g 100 g-1. Palmitoleic and elaidic fatty acids increased linearly as the linseed inclusion in the diet was increased. Polyunsaturated acids increased with the presence of linseed in the diet. For the linolenic acid (C18: 3n3), the best result was with inclusion of 10% of linseed in the total diet. All groups of animals that received linseed obtained better n6:n3 ratios, which varied from 1.81 to 4.14. The higher CLA values obtained in this study are related to the higher amounts of inclusion of linseed in the sheep diet, varying from 1.15 to 5.72 g 100 g-1. It is recommended culling ewes supplemented with inclusion of 10% linseed, for 60 days in feedlot, because they comprise a larger number of favorable traits regarding to the profile fatty acids of Longissimus dorsi.Grãos de linhaça desempenham um papel importante na alimentação dos animais, uma vez que contêm em sua composição, ácidos graxos de cadeia longa que, uma vez absorvidos, são incorporados a carne e ao leite. Para avaliar a concentração de ácidos graxos no músculo Longissimus dorsi de ovelhas de descarte alimentadas com linhaça foram utilizadas 88 ovelhas, com peso corporal inicial médio de 37,65 ± 6,98 kg, distribuídas em delineamento inteiramente casualizado em 12 tratamentos. Os tratamentos constavam da interação entre níveis de linhaça (0, 5, 10 e 15%) e dias de confinamento (30, 45 e 60 dias). Os ácidos graxos observados em maior quantidade na composição do músculo L. dorsi foram o palmítico (27,32 g 100 g-1) e esteárico (17,77 g 100 g-1). Os ácidos saturados mantiveram-se em baixas concentrações na carne quando os animais foram alimentados com maiores teores de linhaça, demonstrando a importância da introdução de alimentos de qualidade na alimentação de animais. O ácido graxo monoinsaturado em maior quantidade foi o ácido oleico com 40 g 100g-1 de ácidos graxos. Os ácidos graxos palmitoléico e elaídico aumentaram linearmente com a inclusão dos grãos de linhaça. Os níveis de ácidos graxos poli-insaturados aumentaram com a presença de grãos de linhaça na ração dieta. Para os ácido linolênico (C18:3n3), o melhor resultado foi com inclusão de 10% de grãos de linhaça na dieta total. Todos os grupos de animais que receberam grãos de linhaça obtiveram melhores proporções de n6:n3, variando entre 1,81 e 4,14. Os maiores valores de CLA obtidos neste trabalho estão relacionados com as maiores taxas de inclusão da linhaça na dieta das ovelhas, variando de 1,15 a 5,72 g 100 g-1. Recomendam-se dietas para ovelhas de descarte com a inclusão de 10% de linhaça por 60 dias em confinamento porque compreenderam um maior número de características favoráveis em relação ao perfil de ácidos graxos no Longissimus dorsi

Evaluation of beetroot (Beta vulgaris L.) leaves during its developmental stages: a chemical composition study

Beetroot leaves (Beta vulgaris L.) are commonly cut off and discarded before using its bulb due to lack of knowledge of how to use them. Aiming at using these leaves, in the present study, in natura and dehydrated beetroot leaves were chemically characterized in terms of fatty acid composition, proximate composition, minerals, total phenolic compounds (TPC), and antioxidant activity by DPPH• in different stages (60, 80, and 100 days) of development. The beetroot leaves showed significant levels of protein and lipids in all developmental stages, and all proximate composition nutrients decreased during these maturation stages; the highest content was observed at 60 days. The Fe content decreased during the developmental stages (from 342.75 to 246.30 mg.kg-1), while the content of K increased (from 13,367.64 to 20,784.90 mg.kg-1). With regard to to fatty acid composition, linolenic acid was present in the greatest quantity, and it increase up to 2.58 mg.g-1 (in natura) and 40.11 mg.g-1 (dehydrated) at 100 days of development. The n-6/n-3 ratios were low in all stages. The TPC and antioxidant activity by DPPH• changed during the developmental stages. The TPC was highest in the 100-day dehydrated leaves (15.27±0.12 mg GAE.g-1 FW), and the 50% inhibition of DPPH• (IC50 89.52 µg.mL-1) were better in the 60-day in natura leaves. This study shows that all developmental stages produced satisfactory results, and therefore, these leaves can be reused as food. The antioxidant activity and the chemical constituents, mainly the ω-3fatty acid, increased during the stages of development

Antioxidant capacity and chemical composition in seeds rich in omega-3: chia, flax, and perilla

The chemical composition and antioxidant capacity of five seeds, chia, golden flax, brown flax, white perilla, and brown perilla, were determined. The chemical properties analyzed included moisture, ash, crude protein, carbohydrates, total lipids, fatty acids, and antioxidant capacity (ABTS•+, DPPH•, and FRAP). The results showed the highest amounts of protein and total lipids in brown and white perilla. Perilla and chia showed higher amounts of alpha-linolenic fatty acid than those of flaxseed varieties, ranging between 531.44 mg g-1 of lipids in brown perilla, 539.07 mg g-1 of lipids in white perilla, and 544.85 mg g-1 of lipis in chia seed. The antioxidant capacity of the seeds, evaluated with ABTS•+, DPPH• , and FRAP methods, showed that brown perilla had greater antioxidant capacity when compared with white perilla, flax, and chia seeds