Majority-rule consensus phylogeny of post-burn trees of <i>Reichardia</i> obtained through Bayesian analysis of the ITS dataset, plotted on geographic map and showing reconstruction of ancestral genome size and chromosome number.

<p>Posterior probabilities are indicated on branches. Values in boxes represent the ancestral genome sizes and their corresponding variances. Dots on the map depict the origin of the sequenced samples. Data on the presence of <i>Reichardia</i> species across Mediterranean countries were retrieved from Euro+Med [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182318#pone.0182318.ref007" target="_blank">7</a>] for <i>R</i>. <i>gaditana</i>, from Blamey and Grey-Wilson [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182318#pone.0182318.ref004" target="_blank">4</a>] for <i>R</i>. <i>intermedia</i> and for <i>R</i>. <i>macrophylla</i> and <i>R</i>. <i>albanica</i> from Conti <i>et al</i>. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182318#pone.0182318.ref001" target="_blank">1</a>].</p

Phylogenetic generalised least squares (PGLS) regression statistics between somatic chromosome number (2n) and other cytogenetic and pollen traits.

<p>Phylogenetic generalised least squares (PGLS) regression statistics between somatic chromosome number (2n) and other cytogenetic and pollen traits.</p

Karyological, cytogenetic and pollen traits plotted on the <i>Reichardia</i> phylogeny.

<p>Posterior probabilities are indicated on branches.</p

DNA content and GC percentage of <i>Reichardia</i> species from different populations.

<p>DNA content and GC percentage of <i>Reichardia</i> species from different populations.</p

Hypothetical schema of the implication of heterochromatin in chromosomal restructuring during reduction of the basic chromosome number and decrease of DNA content.

<p>Hypothetical schema of the implication of heterochromatin in chromosomal restructuring during reduction of the basic chromosome number and decrease of DNA content.</p

Number and distribution of CMA⁺ bands in diploid chromosome set.

<p>Number and distribution of CMA⁺ bands in diploid chromosome set.</p

Number and position of 35S and 5S rDNA loci.

<p>Number and position of 35S and 5S rDNA loci.</p

Hypothetical schema of overarching karyotype evolution in the genus <i>Reichardia</i> involving heterochromatin, rDNA and genome size changes during descending dysploidy.

<p>Hypothetical schema of overarching karyotype evolution in the genus <i>Reichardia</i> involving heterochromatin, rDNA and genome size changes during descending dysploidy.</p

Neighbor-Net of AFLP data obtained from Macaronesian <i>Cheirolophus</i>.

<p>Colour coding profiles delimitate the different species indicating the genetic clusters assigned by BAPS.</p

Geographic location of the 29 sampled populations of Macaronesian <i>Cheirolophus</i> species.

<p>Colour coding circles correspond to genetic structure derived from Bayesian mixture analysis of AFLP markers implemented in BAPS.</p